Novel Method To Detect .beta.-Galactosidase by a Dot-Blotting Assay on Nitrocellulose Membrane Using 6-Bromo-2-naphthyl .beta.-D-Galactopyranoside as Substrate

A novel method to detect beta-galactosidase by dot-blotting samples on nitrocellulose membrane and staining the membrane with azo dye coupling technique using 6-bromo-2-naphthyl beta-D-galactopyranoside as substrate is presented. The enzymatic reaction to split the substrate is pH-dependent, and the...

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