Monoclonal Elisa for 2,4-Dichlorophenoxyacetic Acid: Characterization of Antibodies and Assay Optimization
Twelve monoclonal antibodies (MAbs) against the widely used herbicide 2,4-D were produced by hybridomas from two fusions of murine myeloma cells and spleen cells isolated from BALB/c mice immunized with hapten conjugated via the carboxyl group to thyroglobulin. To evaluate the sensitivity and select...
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| Veröffentlicht in: | Journal of agricultural and food chemistry 1994-06, Vol.42 (6), p.1369-1374 |
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| creator | Franek, Milan Kolar, Vladimir Granatova, Marta Nevorankova, Zora |
| description | Twelve monoclonal antibodies (MAbs) against the widely used herbicide 2,4-D were produced by hybridomas from two fusions of murine myeloma cells and spleen cells isolated from BALB/c mice immunized with hapten conjugated via the carboxyl group to thyroglobulin. To evaluate the sensitivity and selectivity of MAbs, competitive indirect ELISA was used. MAb E2/G2 exhibited the highest sensitivity toward 2,4-D (IC50 = 0.8 ng/mL) and a favorable selectivity toward 18 structurally related substances. Besides the expected highcross-reactivity with methyl ester 2,4-D (104.8%),cross-reactivity with MCPA (13.8%) and with 2,4,5-T (9.5%) was found. Cross-reactivity with other structural analogs did not exceed 2.7%. Optimization studies showed that in competitive ELISAs for 2,4-D coating conjugates with hapten densities of 2.3 and 3.3 mol of 2,4-D/mol of BSA were more sensitive than conjugates with hapten densities of 15.9 and 26.5 mol of 2,4-D/mol of BSA. The best dose-response curves presented in this study were almost linear in the concentration range 0.2-10 ng/mL |
| doi_str_mv | 10.1021/jf00042a024 |
| format | Article |
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To evaluate the sensitivity and selectivity of MAbs, competitive indirect ELISA was used. MAb E2/G2 exhibited the highest sensitivity toward 2,4-D (IC50 = 0.8 ng/mL) and a favorable selectivity toward 18 structurally related substances. Besides the expected highcross-reactivity with methyl ester 2,4-D (104.8%),cross-reactivity with MCPA (13.8%) and with 2,4,5-T (9.5%) was found. Cross-reactivity with other structural analogs did not exceed 2.7%. Optimization studies showed that in competitive ELISAs for 2,4-D coating conjugates with hapten densities of 2.3 and 3.3 mol of 2,4-D/mol of BSA were more sensitive than conjugates with hapten densities of 15.9 and 26.5 mol of 2,4-D/mol of BSA. The best dose-response curves presented in this study were almost linear in the concentration range 0.2-10 ng/mL</description><identifier>ISSN: 0021-8561</identifier><identifier>EISSN: 1520-5118</identifier><identifier>DOI: 10.1021/jf00042a024</identifier><identifier>CODEN: JAFCAU</identifier><language>eng</language><publisher>Washington, DC: American Chemical Society</publisher><subject>2,4-D ; Agronomy. Soil science and plant productions ; Analysis methods ; ANTICORPS MONOCLONAL ; ANTICUERPOS MONOCLONALES ; Applied sciences ; Biological and medical sciences ; ELISA ; Exact sciences and technology ; Fundamental and applied biological sciences. Psychology ; HAPTENE ; HAPTENOS ; Natural water pollution ; Pollution ; REACCION ANTIGENO-ANTICUERPO ; REACTION ANTIGENE ANTICORPS ; Soil and water pollution ; Soil science ; TEST ELISA ; Water treatment and pollution</subject><ispartof>Journal of agricultural and food chemistry, 1994-06, Vol.42 (6), p.1369-1374</ispartof><rights>1995 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a349t-a6e30050d8666113c04a310824eefda3704e333db8f10e02b9ddc802fb4d3eef3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/jf00042a024$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/jf00042a024$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>314,780,784,2756,27067,27915,27916,56729,56779</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3342509$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>Franek, Milan</creatorcontrib><creatorcontrib>Kolar, Vladimir</creatorcontrib><creatorcontrib>Granatova, Marta</creatorcontrib><creatorcontrib>Nevorankova, Zora</creatorcontrib><title>Monoclonal Elisa for 2,4-Dichlorophenoxyacetic Acid: Characterization of Antibodies and Assay Optimization</title><title>Journal of agricultural and food chemistry</title><addtitle>J. Agric. Food Chem</addtitle><description>Twelve monoclonal antibodies (MAbs) against the widely used herbicide 2,4-D were produced by hybridomas from two fusions of murine myeloma cells and spleen cells isolated from BALB/c mice immunized with hapten conjugated via the carboxyl group to thyroglobulin. To evaluate the sensitivity and selectivity of MAbs, competitive indirect ELISA was used. MAb E2/G2 exhibited the highest sensitivity toward 2,4-D (IC50 = 0.8 ng/mL) and a favorable selectivity toward 18 structurally related substances. Besides the expected highcross-reactivity with methyl ester 2,4-D (104.8%),cross-reactivity with MCPA (13.8%) and with 2,4,5-T (9.5%) was found. Cross-reactivity with other structural analogs did not exceed 2.7%. Optimization studies showed that in competitive ELISAs for 2,4-D coating conjugates with hapten densities of 2.3 and 3.3 mol of 2,4-D/mol of BSA were more sensitive than conjugates with hapten densities of 15.9 and 26.5 mol of 2,4-D/mol of BSA. The best dose-response curves presented in this study were almost linear in the concentration range 0.2-10 ng/mL</description><subject>2,4-D</subject><subject>Agronomy. Soil science and plant productions</subject><subject>Analysis methods</subject><subject>ANTICORPS MONOCLONAL</subject><subject>ANTICUERPOS MONOCLONALES</subject><subject>Applied sciences</subject><subject>Biological and medical sciences</subject><subject>ELISA</subject><subject>Exact sciences and technology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>HAPTENE</subject><subject>HAPTENOS</subject><subject>Natural water pollution</subject><subject>Pollution</subject><subject>REACCION ANTIGENO-ANTICUERPO</subject><subject>REACTION ANTIGENE ANTICORPS</subject><subject>Soil and water pollution</subject><subject>Soil science</subject><subject>TEST ELISA</subject><subject>Water treatment and pollution</subject><issn>0021-8561</issn><issn>1520-5118</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><recordid>eNpt0LlLBDEUBvAgCq5HZWeVQrDQ0ZdjjrVb1hPWA9TGJrzN4WYdJ0sygutf78jIYmGV4vu9j_ARssfghAFnp3MHAJIjcLlGBiznkOWMVetkAF2cVXnBNslWSvOOVXkJAzK_DU3QdWiwphe1T0hdiJQfy-zc61kdYljMbBM-l6ht6zUdaW_O6HiGEXVro__C1oeGBkdHTeunwXibKDaGjlLCJb1ftP79F-2QDYd1sru_7zZ5vrx4Gl9nk_urm_FokqGQwzbDwgqAHExVFAVjQoNEwaDi0lpnUJQgrRDCTCvHwAKfDo3RFXA3lUZ0RGyTo75Xx5BStE4ton_HuFQM1M9M6s9MnT7o9QKTxtpFbLRPqxMhJM9h2LGsZz619nMVY3xTRSnKXD09PKry_OX6aszu1E_tfu8dBoWvsat8fhzmIBkUXXjYh6iTmoeP2K2f_v3dNz7Li3k</recordid><startdate>19940601</startdate><enddate>19940601</enddate><creator>Franek, Milan</creator><creator>Kolar, Vladimir</creator><creator>Granatova, Marta</creator><creator>Nevorankova, Zora</creator><general>American Chemical Society</general><scope>FBQ</scope><scope>BSCLL</scope><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>19940601</creationdate><title>Monoclonal Elisa for 2,4-Dichlorophenoxyacetic Acid: Characterization of Antibodies and Assay Optimization</title><author>Franek, Milan ; Kolar, Vladimir ; Granatova, Marta ; Nevorankova, Zora</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a349t-a6e30050d8666113c04a310824eefda3704e333db8f10e02b9ddc802fb4d3eef3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1994</creationdate><topic>2,4-D</topic><topic>Agronomy. Soil science and plant productions</topic><topic>Analysis methods</topic><topic>ANTICORPS MONOCLONAL</topic><topic>ANTICUERPOS MONOCLONALES</topic><topic>Applied sciences</topic><topic>Biological and medical sciences</topic><topic>ELISA</topic><topic>Exact sciences and technology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>HAPTENE</topic><topic>HAPTENOS</topic><topic>Natural water pollution</topic><topic>Pollution</topic><topic>REACCION ANTIGENO-ANTICUERPO</topic><topic>REACTION ANTIGENE ANTICORPS</topic><topic>Soil and water pollution</topic><topic>Soil science</topic><topic>TEST ELISA</topic><topic>Water treatment and pollution</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Franek, Milan</creatorcontrib><creatorcontrib>Kolar, Vladimir</creatorcontrib><creatorcontrib>Granatova, Marta</creatorcontrib><creatorcontrib>Nevorankova, Zora</creatorcontrib><collection>AGRIS</collection><collection>Istex</collection><collection>Pascal-Francis</collection><collection>CrossRef</collection><jtitle>Journal of agricultural and food chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Franek, Milan</au><au>Kolar, Vladimir</au><au>Granatova, Marta</au><au>Nevorankova, Zora</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Monoclonal Elisa for 2,4-Dichlorophenoxyacetic Acid: Characterization of Antibodies and Assay Optimization</atitle><jtitle>Journal of agricultural and food chemistry</jtitle><addtitle>J. Agric. Food Chem</addtitle><date>1994-06-01</date><risdate>1994</risdate><volume>42</volume><issue>6</issue><spage>1369</spage><epage>1374</epage><pages>1369-1374</pages><issn>0021-8561</issn><eissn>1520-5118</eissn><coden>JAFCAU</coden><abstract>Twelve monoclonal antibodies (MAbs) against the widely used herbicide 2,4-D were produced by hybridomas from two fusions of murine myeloma cells and spleen cells isolated from BALB/c mice immunized with hapten conjugated via the carboxyl group to thyroglobulin. To evaluate the sensitivity and selectivity of MAbs, competitive indirect ELISA was used. MAb E2/G2 exhibited the highest sensitivity toward 2,4-D (IC50 = 0.8 ng/mL) and a favorable selectivity toward 18 structurally related substances. Besides the expected highcross-reactivity with methyl ester 2,4-D (104.8%),cross-reactivity with MCPA (13.8%) and with 2,4,5-T (9.5%) was found. Cross-reactivity with other structural analogs did not exceed 2.7%. Optimization studies showed that in competitive ELISAs for 2,4-D coating conjugates with hapten densities of 2.3 and 3.3 mol of 2,4-D/mol of BSA were more sensitive than conjugates with hapten densities of 15.9 and 26.5 mol of 2,4-D/mol of BSA. The best dose-response curves presented in this study were almost linear in the concentration range 0.2-10 ng/mL</abstract><cop>Washington, DC</cop><pub>American Chemical Society</pub><doi>10.1021/jf00042a024</doi><tpages>6</tpages></addata></record> |
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| ispartof | Journal of agricultural and food chemistry, 1994-06, Vol.42 (6), p.1369-1374 |
| issn | 0021-8561 1520-5118 |
| language | eng |
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| source | ACS Publications |
| subjects | 2,4-D Agronomy. Soil science and plant productions Analysis methods ANTICORPS MONOCLONAL ANTICUERPOS MONOCLONALES Applied sciences Biological and medical sciences ELISA Exact sciences and technology Fundamental and applied biological sciences. Psychology HAPTENE HAPTENOS Natural water pollution Pollution REACCION ANTIGENO-ANTICUERPO REACTION ANTIGENE ANTICORPS Soil and water pollution Soil science TEST ELISA Water treatment and pollution |
| title | Monoclonal Elisa for 2,4-Dichlorophenoxyacetic Acid: Characterization of Antibodies and Assay Optimization |
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