An Artificial [Fe 4 S 4 ]-Containing Metalloenzyme for the Reduction of CO 2 to Hydrocarbons

An Artificial [Fe 4 S 4 ]-Containing Metalloenzyme for the Reduction of CO 2 to Hydrocarbons

Iron-sulfur clusters have been reported to catalyze various redox transformations, including the multielectron reduction of CO to hydrocarbons. Herein, we report the design and assembly of an artificial [Fe S ]-containing Fischer-Tropschase relying on the biotin-streptavidin technology. For this pur...

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Veröffentlicht in:Journal of the American Chemical Society 2023-07, Vol.145 (27), p.14823-14830
Hauptverfasser: Waser, Valerie, Mukherjee, Manjistha, Tachibana, Ryo, Igareta, Nico V, Ward, Thomas R
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container_end_page 14830
container_issue 27
container_start_page 14823
container_title Journal of the American Chemical Society
container_volume 145
creator Waser, Valerie
Mukherjee, Manjistha
Tachibana, Ryo
Igareta, Nico V
Ward, Thomas R
description Iron-sulfur clusters have been reported to catalyze various redox transformations, including the multielectron reduction of CO to hydrocarbons. Herein, we report the design and assembly of an artificial [Fe S ]-containing Fischer-Tropschase relying on the biotin-streptavidin technology. For this purpose, we synthesized a bis-biotinylated [Fe S ] cofactor with marked aqueous stability and incorporated it in streptavidin. The effect of the second coordination sphere provided by the protein environment was scrutinized by cyclic voltammetry, highlighting the accessibility of the doubly reduced [Fe S ] cluster. The Fischer-Tropschase activity was improved by chemo-genetic means for the reduction of CO to hydrocarbons with up to 14 turnovers.
doi_str_mv 10.1021/jacs.3c03546
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title An Artificial [Fe 4 S 4 ]-Containing Metalloenzyme for the Reduction of CO 2 to Hydrocarbons
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