Cooperative Nucleophilic and General-Acid Catalysis by the HisH+−His Pair and Arginine Transition State Binding in Catalysis of Ester Hydrolysis Reactions by Designed Helix−Loop−Helix Motifs

A histidine-based two-residue reactive site for the catalysis of hydrolysis and transesterification reactions of p-nitrophenyl esters has been engineered into a helix in a designed helix−loop−helix motif, and it has been shown to function through cooperative nucleophilic and general-acid catalysis....

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Veröffentlicht in:	Journal of the American Chemical Society 1998-05, Vol.120 (17), p.4063-4068
Hauptverfasser:	Broo, Kerstin S, Nilsson, Helena, Nilsson, Jonas, Flodberg, Anna, Baltzer, Lars
Format:	Artikel
Sprache:	eng
Online-Zugang:	Volltext
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