Dynamic Assembly/Disassembly Processes of Photoresponsive DNA Origami Nanostructures Directly Visualized on a Lipid Membrane Surface
Here, we report the direct visualization of the assembly/disassembly processes of photoresponsive DNA origami nanostructures which can be placed on a lipid bilayer surface. The observation relies on controlled interactions between the bilayer components and cholesterol moieties introduced to the hex...
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| Veröffentlicht in: | Journal of the American Chemical Society 2014-02, Vol.136 (5), p.1714-1717 |
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| container_issue | 5 |
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| container_title | Journal of the American Chemical Society |
| container_volume | 136 |
| creator | Suzuki, Yuki Endo, Masayuki Yang, Yangyang Sugiyama, Hiroshi |
| description | Here, we report the direct visualization of the assembly/disassembly processes of photoresponsive DNA origami nanostructures which can be placed on a lipid bilayer surface. The observation relies on controlled interactions between the bilayer components and cholesterol moieties introduced to the hexagonal origami structures, one of whose outer edges carries Azo-ODNs. The bilayer-placed hexagonal dimer was disassembled into monomer units by UV irradiation, and reversibly assembled again during visible light irradiation. These dynamic processes were directly monitored with high-speed atomic force microscopy. The successful application of our approach should facilitate studies of interactive and functional behaviors of various DNA nanostructures. |
| doi_str_mv | 10.1021/ja4109819 |
| format | Article |
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The observation relies on controlled interactions between the bilayer components and cholesterol moieties introduced to the hexagonal origami structures, one of whose outer edges carries Azo-ODNs. The bilayer-placed hexagonal dimer was disassembled into monomer units by UV irradiation, and reversibly assembled again during visible light irradiation. These dynamic processes were directly monitored with high-speed atomic force microscopy. The successful application of our approach should facilitate studies of interactive and functional behaviors of various DNA nanostructures.</description><identifier>ISSN: 0002-7863</identifier><identifier>EISSN: 1520-5126</identifier><identifier>DOI: 10.1021/ja4109819</identifier><identifier>PMID: 24428846</identifier><language>eng</language><publisher>United States: American Chemical Society</publisher><subject>Cholesterol - chemistry ; DNA - chemistry ; DNA - ultrastructure ; Lipid Bilayers - chemistry ; Membranes, Artificial ; Microscopy, Atomic Force ; Nanostructures - chemistry ; Nanostructures - ultrastructure ; Nucleic Acid Conformation ; Photochemical Processes ; Sphingomyelins - chemistry</subject><ispartof>Journal of the American Chemical Society, 2014-02, Vol.136 (5), p.1714-1717</ispartof><rights>Copyright © 2014 American Chemical Society</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a381t-9e4ced32cd3fc54b7f00db0b06c215f0a7a64d7cf75f4861f14738ba583fcb063</citedby><cites>FETCH-LOGICAL-a381t-9e4ced32cd3fc54b7f00db0b06c215f0a7a64d7cf75f4861f14738ba583fcb063</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/ja4109819$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/ja4109819$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>314,776,780,2752,27053,27901,27902,56713,56763</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24428846$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Suzuki, Yuki</creatorcontrib><creatorcontrib>Endo, Masayuki</creatorcontrib><creatorcontrib>Yang, Yangyang</creatorcontrib><creatorcontrib>Sugiyama, Hiroshi</creatorcontrib><title>Dynamic Assembly/Disassembly Processes of Photoresponsive DNA Origami Nanostructures Directly Visualized on a Lipid Membrane Surface</title><title>Journal of the American Chemical Society</title><addtitle>J. Am. Chem. Soc</addtitle><description>Here, we report the direct visualization of the assembly/disassembly processes of photoresponsive DNA origami nanostructures which can be placed on a lipid bilayer surface. The observation relies on controlled interactions between the bilayer components and cholesterol moieties introduced to the hexagonal origami structures, one of whose outer edges carries Azo-ODNs. The bilayer-placed hexagonal dimer was disassembled into monomer units by UV irradiation, and reversibly assembled again during visible light irradiation. These dynamic processes were directly monitored with high-speed atomic force microscopy. The successful application of our approach should facilitate studies of interactive and functional behaviors of various DNA nanostructures.</description><subject>Cholesterol - chemistry</subject><subject>DNA - chemistry</subject><subject>DNA - ultrastructure</subject><subject>Lipid Bilayers - chemistry</subject><subject>Membranes, Artificial</subject><subject>Microscopy, Atomic Force</subject><subject>Nanostructures - chemistry</subject><subject>Nanostructures - ultrastructure</subject><subject>Nucleic Acid Conformation</subject><subject>Photochemical Processes</subject><subject>Sphingomyelins - chemistry</subject><issn>0002-7863</issn><issn>1520-5126</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNptkD1PwzAQhi0EoqUw8AeQFwaGUNtxEnesCF9SaSvxsUYXxwZXTRzZCVKZ-eEYtXRiuvek5x6dXoTOKbmmhNHxCjglE0EnB2hIE0aihLL0EA0JISzKRBoP0In3q7ByJugxGjAeguDpEH3nmwZqI_HUe1WX6804Nx52GS-dlSosHluNlx-2s0751jbefCqcz6d44cx7OMdzaKzvXC-7PhA4N07JLgjejO9hbb5UhW2DAc9Mayr8FOwOGoWfe6dBqlN0pGHt1dlujtDr3e3LzUM0W9w_3kxnEcSCdtFEcamqmMkq1jLhZaYJqUpSklQymmgCGaS8yqTOEs1FSjXlWSxKSETgAxWP0NXWK5313ildtM7U4DYFJcVvk8W-ycBebNm2L2tV7cm_6gJwuQVA-mJle9eE1_8R_QAjVHyi</recordid><startdate>20140205</startdate><enddate>20140205</enddate><creator>Suzuki, Yuki</creator><creator>Endo, Masayuki</creator><creator>Yang, Yangyang</creator><creator>Sugiyama, Hiroshi</creator><general>American Chemical Society</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>20140205</creationdate><title>Dynamic Assembly/Disassembly Processes of Photoresponsive DNA Origami Nanostructures Directly Visualized on a Lipid Membrane Surface</title><author>Suzuki, Yuki ; Endo, Masayuki ; Yang, Yangyang ; Sugiyama, Hiroshi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a381t-9e4ced32cd3fc54b7f00db0b06c215f0a7a64d7cf75f4861f14738ba583fcb063</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Cholesterol - chemistry</topic><topic>DNA - chemistry</topic><topic>DNA - ultrastructure</topic><topic>Lipid Bilayers - chemistry</topic><topic>Membranes, Artificial</topic><topic>Microscopy, Atomic Force</topic><topic>Nanostructures - chemistry</topic><topic>Nanostructures - ultrastructure</topic><topic>Nucleic Acid Conformation</topic><topic>Photochemical Processes</topic><topic>Sphingomyelins - chemistry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Suzuki, Yuki</creatorcontrib><creatorcontrib>Endo, Masayuki</creatorcontrib><creatorcontrib>Yang, Yangyang</creatorcontrib><creatorcontrib>Sugiyama, Hiroshi</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><jtitle>Journal of the American Chemical Society</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Suzuki, Yuki</au><au>Endo, Masayuki</au><au>Yang, Yangyang</au><au>Sugiyama, Hiroshi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Dynamic Assembly/Disassembly Processes of Photoresponsive DNA Origami Nanostructures Directly Visualized on a Lipid Membrane Surface</atitle><jtitle>Journal of the American Chemical Society</jtitle><addtitle>J. Am. Chem. Soc</addtitle><date>2014-02-05</date><risdate>2014</risdate><volume>136</volume><issue>5</issue><spage>1714</spage><epage>1717</epage><pages>1714-1717</pages><issn>0002-7863</issn><eissn>1520-5126</eissn><abstract>Here, we report the direct visualization of the assembly/disassembly processes of photoresponsive DNA origami nanostructures which can be placed on a lipid bilayer surface. The observation relies on controlled interactions between the bilayer components and cholesterol moieties introduced to the hexagonal origami structures, one of whose outer edges carries Azo-ODNs. The bilayer-placed hexagonal dimer was disassembled into monomer units by UV irradiation, and reversibly assembled again during visible light irradiation. These dynamic processes were directly monitored with high-speed atomic force microscopy. The successful application of our approach should facilitate studies of interactive and functional behaviors of various DNA nanostructures.</abstract><cop>United States</cop><pub>American Chemical Society</pub><pmid>24428846</pmid><doi>10.1021/ja4109819</doi><tpages>4</tpages></addata></record> |
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| ispartof | Journal of the American Chemical Society, 2014-02, Vol.136 (5), p.1714-1717 |
| issn | 0002-7863 1520-5126 |
| language | eng |
| recordid | cdi_crossref_primary_10_1021_ja4109819 |
| source | MEDLINE; American Chemical Society Journals |
| subjects | Cholesterol - chemistry DNA - chemistry DNA - ultrastructure Lipid Bilayers - chemistry Membranes, Artificial Microscopy, Atomic Force Nanostructures - chemistry Nanostructures - ultrastructure Nucleic Acid Conformation Photochemical Processes Sphingomyelins - chemistry |
| title | Dynamic Assembly/Disassembly Processes of Photoresponsive DNA Origami Nanostructures Directly Visualized on a Lipid Membrane Surface |
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