Peptide Nanofibers with Dynamic Instability through Nonequilibrium Biocatalytic Assembly
We demonstrate the formation of supramolecular peptide nanofibers that display dynamic instability; i.e., they are formed by competing assembly and disassembly reactions, where assembly is favored away from equilibrium. The systems are based on competitive catalytic transacylation and hydrolysis, pr...
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| Veröffentlicht in: | Journal of the American Chemical Society 2013-11, Vol.135 (45), p.16789-16792 |
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| container_issue | 45 |
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| container_title | Journal of the American Chemical Society |
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| creator | Debnath, Sisir Roy, Sangita Ulijn, Rein V |
| description | We demonstrate the formation of supramolecular peptide nanofibers that display dynamic instability; i.e., they are formed by competing assembly and disassembly reactions, where assembly is favored away from equilibrium. The systems are based on competitive catalytic transacylation and hydrolysis, producing a self-assembling aromatic peptide amphiphile from amino acid precursors that temporarily exceeds the critical gelation concentration, until the competing hydrolytic reaction takes over. Analysis by atomic force microscopy shows consecutive nanofiber formation and shortening. The process results in macroscopically observable temporary hydrogelation, which may be repeated upon refueling the system with further addition of the chemically activated amino acid precursor. Nonequilibrium nanostructures open up opportunities for mimicry of the behavior of dynamic gels found in natural systems and provide components for future adaptive nanotechnologies. |
| doi_str_mv | 10.1021/ja4086353 |
| format | Article |
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The systems are based on competitive catalytic transacylation and hydrolysis, producing a self-assembling aromatic peptide amphiphile from amino acid precursors that temporarily exceeds the critical gelation concentration, until the competing hydrolytic reaction takes over. Analysis by atomic force microscopy shows consecutive nanofiber formation and shortening. The process results in macroscopically observable temporary hydrogelation, which may be repeated upon refueling the system with further addition of the chemically activated amino acid precursor. Nonequilibrium nanostructures open up opportunities for mimicry of the behavior of dynamic gels found in natural systems and provide components for future adaptive nanotechnologies.</description><identifier>ISSN: 0002-7863</identifier><identifier>EISSN: 1520-5126</identifier><identifier>DOI: 10.1021/ja4086353</identifier><identifier>PMID: 24147566</identifier><language>eng</language><publisher>United States: American Chemical Society</publisher><subject>Acylation ; Amino Acids - chemistry ; Biocatalysis ; Gels - chemistry ; Hydrolysis ; Nanofibers - chemistry ; Nanofibers - ultrastructure ; Nanotechnology - methods ; Peptides - chemistry</subject><ispartof>Journal of the American Chemical Society, 2013-11, Vol.135 (45), p.16789-16792</ispartof><rights>Copyright © 2013 American Chemical Society</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a350t-7482ddeadb298aa0e96a28d054c0f915d4644186f1611e10ae0e1d97ffa1f5a33</citedby><cites>FETCH-LOGICAL-a350t-7482ddeadb298aa0e96a28d054c0f915d4644186f1611e10ae0e1d97ffa1f5a33</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/ja4086353$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/ja4086353$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>314,780,784,2765,27076,27924,27925,56738,56788</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24147566$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Debnath, Sisir</creatorcontrib><creatorcontrib>Roy, Sangita</creatorcontrib><creatorcontrib>Ulijn, Rein V</creatorcontrib><title>Peptide Nanofibers with Dynamic Instability through Nonequilibrium Biocatalytic Assembly</title><title>Journal of the American Chemical Society</title><addtitle>J. Am. Chem. Soc</addtitle><description>We demonstrate the formation of supramolecular peptide nanofibers that display dynamic instability; i.e., they are formed by competing assembly and disassembly reactions, where assembly is favored away from equilibrium. The systems are based on competitive catalytic transacylation and hydrolysis, producing a self-assembling aromatic peptide amphiphile from amino acid precursors that temporarily exceeds the critical gelation concentration, until the competing hydrolytic reaction takes over. Analysis by atomic force microscopy shows consecutive nanofiber formation and shortening. The process results in macroscopically observable temporary hydrogelation, which may be repeated upon refueling the system with further addition of the chemically activated amino acid precursor. Nonequilibrium nanostructures open up opportunities for mimicry of the behavior of dynamic gels found in natural systems and provide components for future adaptive nanotechnologies.</description><subject>Acylation</subject><subject>Amino Acids - chemistry</subject><subject>Biocatalysis</subject><subject>Gels - chemistry</subject><subject>Hydrolysis</subject><subject>Nanofibers - chemistry</subject><subject>Nanofibers - ultrastructure</subject><subject>Nanotechnology - methods</subject><subject>Peptides - chemistry</subject><issn>0002-7863</issn><issn>1520-5126</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNptkD1PwzAQhi0EoqUw8AdQFgaGgM-xnXQs5atSVRhAYosusU1d5aPYjlD-PUGFTkynu_fRq9NDyDnQa6AMbjbIaSYTkRyQMQhGYwFMHpIxpZTF6ZCMyIn3m2HlLINjMmIceCqkHJP3F70NVulohU1rbKGdj75sWEd3fYO1LaNF4wMWtrKhj8Latd3HOlq1jf7shlvhbFdHt7YtMWDVh4Gfea_roupPyZHByuuz3zkhbw_3r_OnePn8uJjPljEmgoY45RlTSqMq2DRDpHoqkWWKCl5SMwWhuOQcMmlAAmigqKkGNU2NQTACk2RCrna9pWu9d9rkW2drdH0ONP-xk-_tDOzFjt12Ra3VnvzTMQCXOwBLn2_azjXD6_8UfQPibWxq</recordid><startdate>20131113</startdate><enddate>20131113</enddate><creator>Debnath, Sisir</creator><creator>Roy, Sangita</creator><creator>Ulijn, Rein V</creator><general>American Chemical Society</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>20131113</creationdate><title>Peptide Nanofibers with Dynamic Instability through Nonequilibrium Biocatalytic Assembly</title><author>Debnath, Sisir ; Roy, Sangita ; Ulijn, Rein V</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a350t-7482ddeadb298aa0e96a28d054c0f915d4644186f1611e10ae0e1d97ffa1f5a33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Acylation</topic><topic>Amino Acids - chemistry</topic><topic>Biocatalysis</topic><topic>Gels - chemistry</topic><topic>Hydrolysis</topic><topic>Nanofibers - chemistry</topic><topic>Nanofibers - ultrastructure</topic><topic>Nanotechnology - methods</topic><topic>Peptides - chemistry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Debnath, Sisir</creatorcontrib><creatorcontrib>Roy, Sangita</creatorcontrib><creatorcontrib>Ulijn, Rein V</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><jtitle>Journal of the American Chemical Society</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Debnath, Sisir</au><au>Roy, Sangita</au><au>Ulijn, Rein V</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Peptide Nanofibers with Dynamic Instability through Nonequilibrium Biocatalytic Assembly</atitle><jtitle>Journal of the American Chemical Society</jtitle><addtitle>J. Am. Chem. Soc</addtitle><date>2013-11-13</date><risdate>2013</risdate><volume>135</volume><issue>45</issue><spage>16789</spage><epage>16792</epage><pages>16789-16792</pages><issn>0002-7863</issn><eissn>1520-5126</eissn><abstract>We demonstrate the formation of supramolecular peptide nanofibers that display dynamic instability; i.e., they are formed by competing assembly and disassembly reactions, where assembly is favored away from equilibrium. The systems are based on competitive catalytic transacylation and hydrolysis, producing a self-assembling aromatic peptide amphiphile from amino acid precursors that temporarily exceeds the critical gelation concentration, until the competing hydrolytic reaction takes over. Analysis by atomic force microscopy shows consecutive nanofiber formation and shortening. 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| source | MEDLINE; ACS Publications |
| subjects | Acylation Amino Acids - chemistry Biocatalysis Gels - chemistry Hydrolysis Nanofibers - chemistry Nanofibers - ultrastructure Nanotechnology - methods Peptides - chemistry |
| title | Peptide Nanofibers with Dynamic Instability through Nonequilibrium Biocatalytic Assembly |
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