Structural and conformational study of the aluminum-thymulin complex using 1-D and 2-D NMR techniques
The interaction between aluminum and thymulin, a linear nonapeptide of thymic origin isolated from serum, was investigated by means of one- and two-dimensional NMR experiments. These experiments were performed in dimethyl-d/sub 6/ sulfoxide solution at different metal:peptide ratios. The results lea...
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| Veröffentlicht in: | Inorg. Chem.; (United States) 1988-11, Vol.27 (23), p.4094-4099 |
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| container_title | Inorg. Chem.; (United States) |
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| creator | Laussac, J. P Lefrancier, P Dardenne, M Bach, J. F Marraud, M Cung, M. T |
| description | The interaction between aluminum and thymulin, a linear nonapeptide of thymic origin isolated from serum, was investigated by means of one- and two-dimensional NMR experiments. These experiments were performed in dimethyl-d/sub 6/ sulfoxide solution at different metal:peptide ratios. The results lead the following conclusions: (i) the Al(III) complexation corresponds to a fast exchange on the NMR time scale; (ii) the evolution of /sup 1/H and /sup 13/C NMR chemical shifts indicates the existence of one type of complex with a 1:2 stoichiometry, associating two peptide molecules and one Al(III) ion; (iii) analysis of the spectra suggests that Al(III) has a specific binding site involving the Asn/sup 9/COO/sup /minus// terminal group and the hydroxyl group of the Ser/sup 4/ residue; (iv) from the NOESY data a conformation has been proposed and compared to the biologically active Zn(II)-thymulin complex. 23 refs., 6 figs., 1 tab. |
| doi_str_mv | 10.1021/ic00296a005 |
| format | Article |
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P ; Lefrancier, P ; Dardenne, M ; Bach, J. F ; Marraud, M ; Cung, M. T</creator><creatorcontrib>Laussac, J. P ; Lefrancier, P ; Dardenne, M ; Bach, J. F ; Marraud, M ; Cung, M. T ; CNRS, Toulouse (France)</creatorcontrib><description>The interaction between aluminum and thymulin, a linear nonapeptide of thymic origin isolated from serum, was investigated by means of one- and two-dimensional NMR experiments. These experiments were performed in dimethyl-d/sub 6/ sulfoxide solution at different metal:peptide ratios. The results lead the following conclusions: (i) the Al(III) complexation corresponds to a fast exchange on the NMR time scale; (ii) the evolution of /sup 1/H and /sup 13/C NMR chemical shifts indicates the existence of one type of complex with a 1:2 stoichiometry, associating two peptide molecules and one Al(III) ion; (iii) analysis of the spectra suggests that Al(III) has a specific binding site involving the Asn/sup 9/COO/sup /minus// terminal group and the hydroxyl group of the Ser/sup 4/ residue; (iv) from the NOESY data a conformation has been proposed and compared to the biologically active Zn(II)-thymulin complex. 23 refs., 6 figs., 1 tab.</description><identifier>ISSN: 0020-1669</identifier><identifier>EISSN: 1520-510X</identifier><identifier>DOI: 10.1021/ic00296a005</identifier><identifier>CODEN: INOCAJ</identifier><language>eng</language><publisher>Washington, DC: American Chemical Society</publisher><subject>400201 - Chemical & Physicochemical Properties ; 400202 - Isotope Effects, Isotope Exchange, & Isotope Separation ; ALUMINIUM COMPLEXES ; AROMATICS ; BARYONS ; Biological and medical sciences ; CARBON 13 ; CARBON ISOTOPES ; COMPLEXES ; DATA ; ELEMENTARY PARTICLES ; EVEN-ODD NUCLEI ; EXPERIMENTAL DATA ; FERMIONS ; Fundamental and applied biological sciences. Psychology ; HADRONS ; HYDROXY COMPOUNDS ; INFORMATION ; INORGANIC, ORGANIC, PHYSICAL AND ANALYTICAL CHEMISTRY ; Interactions. Associations ; Intermolecular phenomena ; ISOTOPES ; LIGHT NUCLEI ; MAGNETIC RESONANCE ; Molecular biophysics ; MOLECULAR STRUCTURE ; NUCLEAR MAGNETIC RESONANCE ; NUCLEI ; NUCLEONS ; NUMERICAL DATA ; ORGANIC COMPOUNDS ; PHENOLS ; PROTONS ; RESONANCE ; STABLE ISOTOPES ; THYMOL</subject><ispartof>Inorg. Chem.; (United States), 1988-11, Vol.27 (23), p.4094-4099</ispartof><rights>1989 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a423t-759a94c2f276ecc253d959b62dd3e05482ae6938f369c23fb04485c81919b4813</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/ic00296a005$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/ic00296a005$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>314,776,780,881,2751,27055,27903,27904,56716,56766</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=7251393$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.osti.gov/biblio/5775748$$D View this record in Osti.gov$$Hfree_for_read</backlink></links><search><creatorcontrib>Laussac, J. P</creatorcontrib><creatorcontrib>Lefrancier, P</creatorcontrib><creatorcontrib>Dardenne, M</creatorcontrib><creatorcontrib>Bach, J. F</creatorcontrib><creatorcontrib>Marraud, M</creatorcontrib><creatorcontrib>Cung, M. T</creatorcontrib><creatorcontrib>CNRS, Toulouse (France)</creatorcontrib><title>Structural and conformational study of the aluminum-thymulin complex using 1-D and 2-D NMR techniques</title><title>Inorg. Chem.; (United States)</title><addtitle>Inorg. Chem</addtitle><description>The interaction between aluminum and thymulin, a linear nonapeptide of thymic origin isolated from serum, was investigated by means of one- and two-dimensional NMR experiments. These experiments were performed in dimethyl-d/sub 6/ sulfoxide solution at different metal:peptide ratios. The results lead the following conclusions: (i) the Al(III) complexation corresponds to a fast exchange on the NMR time scale; (ii) the evolution of /sup 1/H and /sup 13/C NMR chemical shifts indicates the existence of one type of complex with a 1:2 stoichiometry, associating two peptide molecules and one Al(III) ion; (iii) analysis of the spectra suggests that Al(III) has a specific binding site involving the Asn/sup 9/COO/sup /minus// terminal group and the hydroxyl group of the Ser/sup 4/ residue; (iv) from the NOESY data a conformation has been proposed and compared to the biologically active Zn(II)-thymulin complex. 23 refs., 6 figs., 1 tab.</description><subject>400201 - Chemical & Physicochemical Properties</subject><subject>400202 - Isotope Effects, Isotope Exchange, & Isotope Separation</subject><subject>ALUMINIUM COMPLEXES</subject><subject>AROMATICS</subject><subject>BARYONS</subject><subject>Biological and medical sciences</subject><subject>CARBON 13</subject><subject>CARBON ISOTOPES</subject><subject>COMPLEXES</subject><subject>DATA</subject><subject>ELEMENTARY PARTICLES</subject><subject>EVEN-ODD NUCLEI</subject><subject>EXPERIMENTAL DATA</subject><subject>FERMIONS</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>HADRONS</subject><subject>HYDROXY COMPOUNDS</subject><subject>INFORMATION</subject><subject>INORGANIC, ORGANIC, PHYSICAL AND ANALYTICAL CHEMISTRY</subject><subject>Interactions. Associations</subject><subject>Intermolecular phenomena</subject><subject>ISOTOPES</subject><subject>LIGHT NUCLEI</subject><subject>MAGNETIC RESONANCE</subject><subject>Molecular biophysics</subject><subject>MOLECULAR STRUCTURE</subject><subject>NUCLEAR MAGNETIC RESONANCE</subject><subject>NUCLEI</subject><subject>NUCLEONS</subject><subject>NUMERICAL DATA</subject><subject>ORGANIC COMPOUNDS</subject><subject>PHENOLS</subject><subject>PROTONS</subject><subject>RESONANCE</subject><subject>STABLE ISOTOPES</subject><subject>THYMOL</subject><issn>0020-1669</issn><issn>1520-510X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1988</creationdate><recordtype>article</recordtype><recordid>eNpt0EtLxDAQB_AgCq6rJ79AEcGDVPNo0uYovt-LruAtZNPUjbbpmge4395oRTx4Gpj8Msz8AdhG8ABBjA6NghBzJiGkK2CEKIY5RfB5FYxSH-aIMb4ONrx_hRByUrAR0I_BRRWik20mbZ2p3ja962QwvU0tH2K9zPomC3OdyTZ2xsYuD_NlF1tjk-4Wrf7Iojf2JUP5yfcMnOrd7UMWtJpb8x613wRrjWy93vqpY_B0djo9vshv7s8vj49ucllgEvKScskLhRtcMq0UpqTmlM8YrmuiIS0qLDXjpGoI4wqTZgaLoqKqQhzxWVEhMgY7w9zeByO8Ml8rpJOsVkHQsqRlUSW0PyDleu-dbsTCmU66pUBQfMUo_sSY9O6gF9Ir2TZOWmX875cSU0Q4SSwfmPFBf_w-S_cmWElKKqaTR4En12hC0VRcJb83eKm8eO2jS2n7fxf4BEi9jD8</recordid><startdate>19881101</startdate><enddate>19881101</enddate><creator>Laussac, J. P</creator><creator>Lefrancier, P</creator><creator>Dardenne, M</creator><creator>Bach, J. F</creator><creator>Marraud, M</creator><creator>Cung, M. T</creator><general>American Chemical Society</general><scope>BSCLL</scope><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>OTOTI</scope></search><sort><creationdate>19881101</creationdate><title>Structural and conformational study of the aluminum-thymulin complex using 1-D and 2-D NMR techniques</title><author>Laussac, J. P ; Lefrancier, P ; Dardenne, M ; Bach, J. F ; Marraud, M ; Cung, M. T</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a423t-759a94c2f276ecc253d959b62dd3e05482ae6938f369c23fb04485c81919b4813</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1988</creationdate><topic>400201 - Chemical & Physicochemical Properties</topic><topic>400202 - Isotope Effects, Isotope Exchange, & Isotope Separation</topic><topic>ALUMINIUM COMPLEXES</topic><topic>AROMATICS</topic><topic>BARYONS</topic><topic>Biological and medical sciences</topic><topic>CARBON 13</topic><topic>CARBON ISOTOPES</topic><topic>COMPLEXES</topic><topic>DATA</topic><topic>ELEMENTARY PARTICLES</topic><topic>EVEN-ODD NUCLEI</topic><topic>EXPERIMENTAL DATA</topic><topic>FERMIONS</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>HADRONS</topic><topic>HYDROXY COMPOUNDS</topic><topic>INFORMATION</topic><topic>INORGANIC, ORGANIC, PHYSICAL AND ANALYTICAL CHEMISTRY</topic><topic>Interactions. Associations</topic><topic>Intermolecular phenomena</topic><topic>ISOTOPES</topic><topic>LIGHT NUCLEI</topic><topic>MAGNETIC RESONANCE</topic><topic>Molecular biophysics</topic><topic>MOLECULAR STRUCTURE</topic><topic>NUCLEAR MAGNETIC RESONANCE</topic><topic>NUCLEI</topic><topic>NUCLEONS</topic><topic>NUMERICAL DATA</topic><topic>ORGANIC COMPOUNDS</topic><topic>PHENOLS</topic><topic>PROTONS</topic><topic>RESONANCE</topic><topic>STABLE ISOTOPES</topic><topic>THYMOL</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Laussac, J. P</creatorcontrib><creatorcontrib>Lefrancier, P</creatorcontrib><creatorcontrib>Dardenne, M</creatorcontrib><creatorcontrib>Bach, J. F</creatorcontrib><creatorcontrib>Marraud, M</creatorcontrib><creatorcontrib>Cung, M. T</creatorcontrib><creatorcontrib>CNRS, Toulouse (France)</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>OSTI.GOV</collection><jtitle>Inorg. Chem.; (United States)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Laussac, J. P</au><au>Lefrancier, P</au><au>Dardenne, M</au><au>Bach, J. F</au><au>Marraud, M</au><au>Cung, M. T</au><aucorp>CNRS, Toulouse (France)</aucorp><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Structural and conformational study of the aluminum-thymulin complex using 1-D and 2-D NMR techniques</atitle><jtitle>Inorg. Chem.; (United States)</jtitle><addtitle>Inorg. Chem</addtitle><date>1988-11-01</date><risdate>1988</risdate><volume>27</volume><issue>23</issue><spage>4094</spage><epage>4099</epage><pages>4094-4099</pages><issn>0020-1669</issn><eissn>1520-510X</eissn><coden>INOCAJ</coden><abstract>The interaction between aluminum and thymulin, a linear nonapeptide of thymic origin isolated from serum, was investigated by means of one- and two-dimensional NMR experiments. These experiments were performed in dimethyl-d/sub 6/ sulfoxide solution at different metal:peptide ratios. The results lead the following conclusions: (i) the Al(III) complexation corresponds to a fast exchange on the NMR time scale; (ii) the evolution of /sup 1/H and /sup 13/C NMR chemical shifts indicates the existence of one type of complex with a 1:2 stoichiometry, associating two peptide molecules and one Al(III) ion; (iii) analysis of the spectra suggests that Al(III) has a specific binding site involving the Asn/sup 9/COO/sup /minus// terminal group and the hydroxyl group of the Ser/sup 4/ residue; (iv) from the NOESY data a conformation has been proposed and compared to the biologically active Zn(II)-thymulin complex. 23 refs., 6 figs., 1 tab.</abstract><cop>Washington, DC</cop><pub>American Chemical Society</pub><doi>10.1021/ic00296a005</doi><tpages>6</tpages></addata></record> |
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| subjects | 400201 - Chemical & Physicochemical Properties 400202 - Isotope Effects, Isotope Exchange, & Isotope Separation ALUMINIUM COMPLEXES AROMATICS BARYONS Biological and medical sciences CARBON 13 CARBON ISOTOPES COMPLEXES DATA ELEMENTARY PARTICLES EVEN-ODD NUCLEI EXPERIMENTAL DATA FERMIONS Fundamental and applied biological sciences. Psychology HADRONS HYDROXY COMPOUNDS INFORMATION INORGANIC, ORGANIC, PHYSICAL AND ANALYTICAL CHEMISTRY Interactions. Associations Intermolecular phenomena ISOTOPES LIGHT NUCLEI MAGNETIC RESONANCE Molecular biophysics MOLECULAR STRUCTURE NUCLEAR MAGNETIC RESONANCE NUCLEI NUCLEONS NUMERICAL DATA ORGANIC COMPOUNDS PHENOLS PROTONS RESONANCE STABLE ISOTOPES THYMOL |
| title | Structural and conformational study of the aluminum-thymulin complex using 1-D and 2-D NMR techniques |
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