Core-Directed Protein Design. II. Rescue of a Multiply Mutated and Destabilized Variant of Ubiquitin

We have applied the method described in the preceding paper [Finucane, M. D., et al. (1999) Biochemistry 38, 11604−11612], namely, stability-based selection using phage display, to explore the sequence requirements for packing in the hydrophobic core of ubiquitin. In contrast to the parent protein,...

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Veröffentlicht in:	Biochemistry (Easton) 1999-09, Vol.38 (36), p.11613-11623
Hauptverfasser:	Finucane, Michael D, Woolfson, Derek N
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Animals Base Sequence Cattle Circular Dichroism DNA Primers Hot Temperature Humans Magnetic Resonance Spectroscopy Molecular Sequence Data Mutagenesis, Site-Directed Protein Conformation Sequence Homology, Amino Acid Ubiquitins - chemistry Ubiquitins - genetics
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