Slowed Enzymatic Turnover Allows Characterization of Intermediates by Solid-State NMR

EPSP (5-enolpyruvylshikimate-3-phosphate) synthase catalyzes condensation of shikimate 3-phosphate (S3P) and phosphoenolpyruvate (PEP) to form EPSP, a precursor to the aromatic amino acids. S3P and [2-13C]PEP were bound to mutant or wild type E. coli forms of the enzyme prior to lyophilization. CPMA...

Ausführliche Beschreibung