Functional Identification of Calcium Binding Residues in Bovine α-Lactalbumin

The functional role of previously identified calcium binding residues in α-lactalbumin (α-LA) was investigated by site-directed mutagenesis. Mutation of D82 to alanine did not effect the binding affinity for calcium, the protein structure, or its function in the lactose synthase assay, suggesting th...

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Veröffentlicht in:	Biochemistry (Easton) 1997-09, Vol.36 (39), p.11648-11654
Hauptverfasser:	Anderson, Patricia J, Brooks, Charles L, Berliner, Lawrence J
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Binding Sites BULLS CALCIO CALCIUM Calcium - metabolism Cattle Circular Dichroism Crystallography, X-Ray Humans Lactalbumin - metabolism Models, Molecular Protein Conformation TAUREAU TORO
Online-Zugang:	Volltext
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