Phosphatidylinositol-Specific Phospholipase C: Kinetic and Stereochemical Evidence for an Interaction between Arginine-69 and the Phosphate Group of Phosphatidylinositol

A new substrate analogue, (2R)-1,2-dipalmitoyloxypropanethiophospho-1-d-myo-inositol (DPsPI), has been used in a new, continuous assay for phosphatidylinositol-specific phospholipase C (PI-PLC). DPsPI is superior to other substrate analogs that have been used for assaying PI-PLC since it is synthesi...

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Veröffentlicht in:	Biochemistry (Easton) 1997-06, Vol.36 (22), p.6633-6642
Hauptverfasser:	Hondal, Robert J, Riddle, Suzette R, Kravchuk, Alexander V, Zhao, Zhong, Liao, Hua, Bruzik, Karol S, Tsai, Ming-Daw
Format:	Artikel
Sprache:	eng
Schlagworte:	Arginine - metabolism Bacillus thuringiensis - enzymology Binding Sites Catalysis Circular Dichroism Escherichia coli - genetics Gene Expression Kinetics Magnetic Resonance Spectroscopy Mutagenesis Organothiophosphates - chemistry Organothiophosphates - metabolism Phosphates - metabolism Phosphatidylinositol Diacylglycerol-Lyase Phosphatidylinositols - chemistry Phosphatidylinositols - metabolism Phosphoinositide Phospholipase C Phosphoric Diester Hydrolases - chemistry Phosphoric Diester Hydrolases - genetics Phosphoric Diester Hydrolases - metabolism Protein Conformation Recombinant Proteins Stereoisomerism Structure-Activity Relationship Thermodynamics
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