Two Secondary Carbohydrate Binding Sites on the Surface of Barley α-Amylase 1 Have Distinct Functions and Display Synergy in Hydrolysis of Starch Granules

Some polysaccharide processing enzymes possess secondary carbohydrate binding sites situated on the surface far from the active site. In barley α-amylase 1 (AMY1), two such sites, SBS1 and SBS2, are found on the catalytic (β/α)8-barrel and the noncatalytic C-terminal domain, respectively. Site-direc...

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