Kinetic Mechanism and Inhibitor Characterization of WNK1 Kinase

Pseudohypoaldosteronism type II (PHAII) is caused by the mutation of two members of the WNK (with-no-K[Lys] kinase) kinase family. We describe here the development of an in vitro WNK1 microfluidic mobility shift assay for kinetic mechanism studies. Assays using capillary electrophoresis on a microfl...

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Veröffentlicht in:	Biochemistry (Easton) 2009-11, Vol.48 (43), p.10255-10266
Hauptverfasser:	Yagi, Yukiko I, Abe, Koichi, Ikebukuro, Kazunori, Sode, Koji
Format:	Artikel
Sprache:	eng
Schlagworte:	Electrophoretic Mobility Shift Assay Enzyme Inhibitors - pharmacology Humans Inhibitory Concentration 50 Intracellular Signaling Peptides and Proteins Kinetics Minor Histocompatibility Antigens Protein-Serine-Threonine Kinases - antagonists & inhibitors Protein-Serine-Threonine Kinases - metabolism Pyrazoles - pharmacology Pyrimidines - pharmacology Staurosporine - pharmacology Tyrphostins - pharmacology WNK Lysine-Deficient Protein Kinase 1
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