Ultraviolet photoinactivation of galactosyltransferase. Protection by substrates
Galactosyltransferase was irreversibly inactivated upon exposure to ultraviolet light and the rate of inactivation followed apparent first-order kinetics. Significant protection against inactivation was observed in the presence of various combinations of substrates. UDPgalactose and Mn2+ together ga...
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| Veröffentlicht in: | Biochemistry (Easton) 1976-03, Vol.15 (5), p.1093-1097 |
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| container_issue | 5 |
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| container_title | Biochemistry (Easton) |
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| creator | Clymer, David J Geren, Collis R Ebner, Kurt E |
| description | Galactosyltransferase was irreversibly inactivated upon exposure to ultraviolet light and the rate of inactivation followed apparent first-order kinetics. Significant protection against inactivation was observed in the presence of various combinations of substrates. UDPgalactose and Mn2+ together gave the most protection. Amino acid analyses revealed the loss of 1 mol of tryptophan per mol of galactosyltransferase upon ultraviolet photoinactivation. Further evidence for an essential trypotphan was provided by difference spectra and by inactivation with 2-hydroxy-5-nitrobenzyl bromide and protection against this reagent by Mn2+ and UDPgalactose. The protection by UDPgalactose and Mn2+ was greater than that provided by UDPgalactose alone. Since Mn2+ provided no protection by itself, this suggested that the formation of the galactosyltransferase-Mn2+-UDPgalactose complex caused a conformational change which was responsible for the observed protection of the essential tryptophanyl residue. |
| doi_str_mv | 10.1021/bi00650a022 |
| format | Article |
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The protection by UDPgalactose and Mn2+ was greater than that provided by UDPgalactose alone. Since Mn2+ provided no protection by itself, this suggested that the formation of the galactosyltransferase-Mn2+-UDPgalactose complex caused a conformational change which was responsible for the observed protection of the essential tryptophanyl residue.</description><identifier>ISSN: 0006-2960</identifier><identifier>EISSN: 1520-4995</identifier><identifier>DOI: 10.1021/bi00650a022</identifier><identifier>PMID: 1252429</identifier><language>eng</language><publisher>United States: American Chemical Society</publisher><subject>560111 -Biomedical Sciences, Applied Studies-Radiation Effects-Radiation Effects on Biochemicals-In Vitro ; Amino Acids - analysis ; Animals ; Cattle ; Female ; GALACTOSE- RADIOSENSITIVITY EFFECTS ; INACTIVATION ; IRRADIATION ; Kinetics ; Manganese - pharmacology ; MANGANESE COMPOUNDS- RADIOSENSITIVITY EFFECTS ; Milk - enzymology ; Molecular Weight ; N48110 -Life Sciences-Radiation Effects on Biochemicals- In-Vitro ; Radiation Effects ; Spectrometry, Fluorescence ; Spectrophotometry, Ultraviolet ; TRANSFERASES- BIOLOGICAL RADIATION EFFECTS ; Tryptophan - analysis ; ULTRAVIOLET RADIATION ; Ultraviolet Rays ; Uridine Diphosphate Galactose - pharmacology ; Uridine Diphosphate Sugars - pharmacology</subject><ispartof>Biochemistry (Easton), 1976-03, Vol.15 (5), p.1093-1097</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a381t-b307b93ced14c334ccd64296f06daa513490a915ba82cdc552278588488162363</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/bi00650a022$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/bi00650a022$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>314,780,784,885,2765,27076,27924,27925,56738,56788</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1252429$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://www.osti.gov/biblio/4018328$$D View this record in Osti.gov$$Hfree_for_read</backlink></links><search><creatorcontrib>Clymer, David J</creatorcontrib><creatorcontrib>Geren, Collis R</creatorcontrib><creatorcontrib>Ebner, Kurt E</creatorcontrib><creatorcontrib>Univ. of Kansas, Kansas City</creatorcontrib><title>Ultraviolet photoinactivation of galactosyltransferase. Protection by substrates</title><title>Biochemistry (Easton)</title><addtitle>Biochemistry</addtitle><description>Galactosyltransferase was irreversibly inactivated upon exposure to ultraviolet light and the rate of inactivation followed apparent first-order kinetics. Significant protection against inactivation was observed in the presence of various combinations of substrates. UDPgalactose and Mn2+ together gave the most protection. Amino acid analyses revealed the loss of 1 mol of tryptophan per mol of galactosyltransferase upon ultraviolet photoinactivation. Further evidence for an essential trypotphan was provided by difference spectra and by inactivation with 2-hydroxy-5-nitrobenzyl bromide and protection against this reagent by Mn2+ and UDPgalactose. The protection by UDPgalactose and Mn2+ was greater than that provided by UDPgalactose alone. Since Mn2+ provided no protection by itself, this suggested that the formation of the galactosyltransferase-Mn2+-UDPgalactose complex caused a conformational change which was responsible for the observed protection of the essential tryptophanyl residue.</description><subject>560111 -Biomedical Sciences, Applied Studies-Radiation Effects-Radiation Effects on Biochemicals-In Vitro</subject><subject>Amino Acids - analysis</subject><subject>Animals</subject><subject>Cattle</subject><subject>Female</subject><subject>GALACTOSE- RADIOSENSITIVITY EFFECTS</subject><subject>INACTIVATION</subject><subject>IRRADIATION</subject><subject>Kinetics</subject><subject>Manganese - pharmacology</subject><subject>MANGANESE COMPOUNDS- RADIOSENSITIVITY EFFECTS</subject><subject>Milk - enzymology</subject><subject>Molecular Weight</subject><subject>N48110 -Life Sciences-Radiation Effects on Biochemicals- In-Vitro</subject><subject>Radiation Effects</subject><subject>Spectrometry, Fluorescence</subject><subject>Spectrophotometry, Ultraviolet</subject><subject>TRANSFERASES- BIOLOGICAL RADIATION EFFECTS</subject><subject>Tryptophan - analysis</subject><subject>ULTRAVIOLET RADIATION</subject><subject>Ultraviolet Rays</subject><subject>Uridine Diphosphate Galactose - pharmacology</subject><subject>Uridine Diphosphate Sugars - pharmacology</subject><issn>0006-2960</issn><issn>1520-4995</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1976</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNptkM9LwzAYhoMoc05PnoXixYN05mebHmU6FQYOt51DmqYuszYjyYb7782sqAdP4cvz8H0vLwDnCA4RxOimNBBmDEqI8QHoI4ZhSouCHYI-jCDFRQaPwYn3qzhSmNMe6CHMMMVFH0wXTXBya2yjQ7Je2mBNK1UwWxmMbRNbJ6-yiR_W7_Zi62vtpNfDZOps0OpLKneJ35Q-4qD9KTiqZeP12fc7AIvx_Xz0mE6eH55Gt5NUEo5CWhKYlwVRukJUEUKVqrIYKKthVknJEKEFlAVipeRYVYoxjHPOOKecowyTjAzAZbfX-mCEVyamWSrbtjGUoBBxgnmUrjtJOeu907VYO_Mu3U4gKPbdiT_dRfuis9eb8l1Xv25XVuRpx40P-uMHS_cmspzkTMynM_GCyd0kn43EOPpXnS-VFyu7cW0s5N_Ln3a4hRA</recordid><startdate>19760301</startdate><enddate>19760301</enddate><creator>Clymer, David J</creator><creator>Geren, Collis R</creator><creator>Ebner, Kurt E</creator><general>American Chemical Society</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>OTOTI</scope></search><sort><creationdate>19760301</creationdate><title>Ultraviolet photoinactivation of galactosyltransferase. Protection by substrates</title><author>Clymer, David J ; Geren, Collis R ; Ebner, Kurt E</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a381t-b307b93ced14c334ccd64296f06daa513490a915ba82cdc552278588488162363</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1976</creationdate><topic>560111 -Biomedical Sciences, Applied Studies-Radiation Effects-Radiation Effects on Biochemicals-In Vitro</topic><topic>Amino Acids - analysis</topic><topic>Animals</topic><topic>Cattle</topic><topic>Female</topic><topic>GALACTOSE- RADIOSENSITIVITY EFFECTS</topic><topic>INACTIVATION</topic><topic>IRRADIATION</topic><topic>Kinetics</topic><topic>Manganese - pharmacology</topic><topic>MANGANESE COMPOUNDS- RADIOSENSITIVITY EFFECTS</topic><topic>Milk - enzymology</topic><topic>Molecular Weight</topic><topic>N48110 -Life Sciences-Radiation Effects on Biochemicals- In-Vitro</topic><topic>Radiation Effects</topic><topic>Spectrometry, Fluorescence</topic><topic>Spectrophotometry, Ultraviolet</topic><topic>TRANSFERASES- BIOLOGICAL RADIATION EFFECTS</topic><topic>Tryptophan - analysis</topic><topic>ULTRAVIOLET RADIATION</topic><topic>Ultraviolet Rays</topic><topic>Uridine Diphosphate Galactose - pharmacology</topic><topic>Uridine Diphosphate Sugars - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Clymer, David J</creatorcontrib><creatorcontrib>Geren, Collis R</creatorcontrib><creatorcontrib>Ebner, Kurt E</creatorcontrib><creatorcontrib>Univ. of Kansas, Kansas City</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>OSTI.GOV</collection><jtitle>Biochemistry (Easton)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Clymer, David J</au><au>Geren, Collis R</au><au>Ebner, Kurt E</au><aucorp>Univ. of Kansas, Kansas City</aucorp><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Ultraviolet photoinactivation of galactosyltransferase. Protection by substrates</atitle><jtitle>Biochemistry (Easton)</jtitle><addtitle>Biochemistry</addtitle><date>1976-03-01</date><risdate>1976</risdate><volume>15</volume><issue>5</issue><spage>1093</spage><epage>1097</epage><pages>1093-1097</pages><issn>0006-2960</issn><eissn>1520-4995</eissn><abstract>Galactosyltransferase was irreversibly inactivated upon exposure to ultraviolet light and the rate of inactivation followed apparent first-order kinetics. Significant protection against inactivation was observed in the presence of various combinations of substrates. UDPgalactose and Mn2+ together gave the most protection. Amino acid analyses revealed the loss of 1 mol of tryptophan per mol of galactosyltransferase upon ultraviolet photoinactivation. Further evidence for an essential trypotphan was provided by difference spectra and by inactivation with 2-hydroxy-5-nitrobenzyl bromide and protection against this reagent by Mn2+ and UDPgalactose. The protection by UDPgalactose and Mn2+ was greater than that provided by UDPgalactose alone. Since Mn2+ provided no protection by itself, this suggested that the formation of the galactosyltransferase-Mn2+-UDPgalactose complex caused a conformational change which was responsible for the observed protection of the essential tryptophanyl residue.</abstract><cop>United States</cop><pub>American Chemical Society</pub><pmid>1252429</pmid><doi>10.1021/bi00650a022</doi><tpages>5</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0006-2960 |
| ispartof | Biochemistry (Easton), 1976-03, Vol.15 (5), p.1093-1097 |
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| language | eng |
| recordid | cdi_crossref_primary_10_1021_bi00650a022 |
| source | MEDLINE; American Chemical Society Journals |
| subjects | 560111 -Biomedical Sciences, Applied Studies-Radiation Effects-Radiation Effects on Biochemicals-In Vitro Amino Acids - analysis Animals Cattle Female GALACTOSE- RADIOSENSITIVITY EFFECTS INACTIVATION IRRADIATION Kinetics Manganese - pharmacology MANGANESE COMPOUNDS- RADIOSENSITIVITY EFFECTS Milk - enzymology Molecular Weight N48110 -Life Sciences-Radiation Effects on Biochemicals- In-Vitro Radiation Effects Spectrometry, Fluorescence Spectrophotometry, Ultraviolet TRANSFERASES- BIOLOGICAL RADIATION EFFECTS Tryptophan - analysis ULTRAVIOLET RADIATION Ultraviolet Rays Uridine Diphosphate Galactose - pharmacology Uridine Diphosphate Sugars - pharmacology |
| title | Ultraviolet photoinactivation of galactosyltransferase. Protection by substrates |
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