Synthesis of prodan-phosphatidylcholine, a new fluorescent probe, and its interactions with pancreatic and snake venom phospholipases A2

A new fluorescent probe, prodan-PC, was synthesized by incubating thio-PC, a thiol ester analogue of phosphatidylcholine [1,2-bis(decanoylthio)-1,2-dideoxy-sn-glycero-3-phosphocholine], with acrylodan, a fluorescent thiol-reactive reagent [6-acryloyl-2-(dimethylamino)naphthalene], in the presence of...

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Veröffentlicht in:	Biochemistry (Easton) 1987-06, Vol.26 (12), p.3697-3703
Hauptverfasser:	Hendrickson, H. S, Dumdei, E. J, Batchelder, A. G, Carlson, G. L
Format:	Artikel
Sprache:	eng
Schlagworte:	2-Naphthylamine - analogs & derivatives 2-Naphthylamine - chemical synthesis 2-Naphthylamine - pharmacology Animals Biological and medical sciences Crotalid Venoms Elapid Venoms Fluorescence Polarization Fluorescent Dyes - chemical synthesis Fundamental and applied biological sciences. Psychology Indicators and Reagents Interactions. Associations Intermolecular phenomena Kinetics Molecular biophysics Naphthalenes - chemical synthesis Pancreas - enzymology Phosphatidylcholines - chemical synthesis Phosphatidylcholines - pharmacology Phospholipases - metabolism Phospholipases A - metabolism Phospholipases A2 Spectrometry, Fluorescence Swine
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