The Role of Active Site Residue Arginine 218 in Firefly Luciferase Bioluminescence

Firefly luciferase catalyzes the highly efficient emission of yellow−green light from substrate firefly luciferin by a sequence of reactions that require Mg-ATP and molecular oxygen. We had previously developed a working model of the luciferase active site based on the X-ray structure of the enzyme...

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Veröffentlicht in:	Biochemistry (Easton) 2001-02, Vol.40 (8), p.2410-2418
Hauptverfasser:	Branchini, Bruce R, Magyar, Rachelle A, Murtiashaw, Martha H, Portier, Nathan C
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Animals Arginine - chemistry Arginine - genetics Arginine - metabolism Binding Sites - genetics Catalysis Coleoptera - enzymology Genetic Vectors - chemical synthesis Genetic Vectors - metabolism Kinetics Luciferases - biosynthesis Luciferases - chemistry Luciferases - isolation & purification Luciferases - metabolism Luminescent Measurements Molecular Sequence Data Mutagenesis, Site-Directed Oxidation-Reduction Recombinant Proteins - biosynthesis Recombinant Proteins - chemistry Recombinant Proteins - isolation & purification Substrate Specificity - genetics
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