Calcium Binding to the Regulatory N-Domain of Skeletal Muscle Troponin C Occurs in a Stepwise Manner

Ca2+ binding to a recombinant regulatory N-domain (residues 1-90) of chicken troponin C (NTnC) has been investigated with the use of heteronuclear multidimensional NMR spectroscopy. The protein has been cloned in pET3a vector and expressed in minimal media in Escherichia coli to allow uniform 15N an...

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Veröffentlicht in:	Biochemistry (Easton) 1995-07, Vol.34 (26), p.8330-8340
Hauptverfasser:	Li, Monica X, Gagne, Stephane M, Tsuda, Sakae, Kay, Cyril M, Smillie, Lawrence B, Sykes, Brian D
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Animals Binding Sites Calcium - metabolism Chickens Circular Dichroism Cloning, Molecular Escherichia coli Kinetics Magnetic Resonance Spectroscopy Molecular Sequence Data Muscle, Skeletal - metabolism Mutagenesis, Site-Directed Point Mutation Protein Conformation Recombinant Proteins - chemistry Recombinant Proteins - metabolism Troponin - chemistry Troponin - metabolism Troponin C
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