Azotobacter vinelandii Nitrogenases with Substitutions in the FeMo-Cofactor Environment of the MoFe Protein:  Effects of Acetylene or Ethylene on Interactions with H+, HCN, and CN-

Wild-type and three altered Azotobacter vinelandii nitrogenase MoFe proteins, with substitutions either at α-195His (replaced by α-195Asn or α-195Gln) or at α-191Gln (replaced by α-191Lys), were used to probe the interactions of HCN and CN-, both of which are present in NaCN solutions at pH 7.4, wit...

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Veröffentlicht in:Biochemistry (Easton) 2000-09, Vol.39 (35), p.10855-10865
Hauptverfasser: Fisher, Karl, Dilworth, Michael J, Kim, Chul-Hwan, Newton, William E
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Sprache:eng
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