Overcoming the Challenges of Megabase-Sized Plasmid Construction in Escherichia coli

Although has been a popular tool for plasmid construction, this bacterium was believed to be "unsuitable" for constructing a large plasmid whose size exceeds 500 kilobases. We assumed that traditional plasmid vectors may lack some regulatory DNA elements required for the stable replication...

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Veröffentlicht in:	ACS synthetic biology 2020-06, Vol.9 (6), p.1315-1327
Hauptverfasser:	Mukai, Takahito, Yoneji, Tatsuya, Yamada, Kayoko, Fujita, Hironobu, Nara, Seia, Su'etsugu, Masayuki
Format:	Artikel
Sprache:	eng
Schlagworte:	Chloramphenicol - pharmacology Chromosomes, Artificial, Bacterial - genetics DNA Replication - drug effects Escherichia coli - metabolism Genetic Engineering - methods Genetic Vectors - genetics Genetic Vectors - metabolism Recombination, Genetic Vibrio - genetics
Online-Zugang:	Volltext
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