Application of a Colorimetric and Near-Infrared Fluorescent Probe in Peroxynitrite Detection and Imaging in Living Cells
Peroxynitrite (ONOO–) plays a vital role in pathological and physiological processes, and an excessive amount of ONOO– causes various diseases. Developing a specific and sensitive method for the detection of ONOO– in biological systems is significant. Herein, we reported a novel colorimetric and nea...
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| creator | Gu, Biao Liu, Cunfei Wu, Yang Zhang, Chunxiang Shen, Youming Liu, Mengqin |
| description | Peroxynitrite (ONOO–) plays a vital role in pathological and physiological processes, and an excessive amount of ONOO– causes various diseases. Developing a specific and sensitive method for the detection of ONOO– in biological systems is significant. Herein, we reported a novel colorimetric and near-infrared fluorescent probe (pyridin-4-ylmethyl (Z)-2-cyano-2-(3-((E)-4-hydroxystyryl)-5,5-dimethylcyclohex-2-en-1-ylidene)acetate diphenyl phosphinate group (AN-DP)) based on isophorone and phosphinate groups for ONOO– detection. The probe displayed excellent selectivity toward ONOO– compared with other relevant analytes. It showed a good linear relationship between the fluorescence intensity at 670 nm and ONOO– concentration (0–10 μM) with a low detection limit (53 nM). Importantly, the probe was a colorimetric and near-infrared fluorescent probe suitable for ONOO– detection. Furthermore, the probe could be used for imaging ONOO– in HepG2 cells. |
| doi_str_mv | 10.1021/acsomega.0c04073 |
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| subjects | Chemistry Chemistry, Multidisciplinary Physical Sciences Science & Technology |
| title | Application of a Colorimetric and Near-Infrared Fluorescent Probe in Peroxynitrite Detection and Imaging in Living Cells |
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