Nature-Inspired DNA Nanosensor for Real-Time in Situ Detection of mRNA in Living Cells
Rapid and precise in situ detection of gene expressions within a single cell is highly informative and offers valuable insights into its state. Detecting mRNA within single cells in real time and nondestructively remains an important challenge. Using DNA nanotechnology and inspired by nature’s many...
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| Veröffentlicht in: | ACS nano 2015-05, Vol.9 (5), p.5609-5617 |
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| creator | Tay, Chor Yong Yuan, Liang Leong, David Tai |
| description | Rapid and precise in situ detection of gene expressions within a single cell is highly informative and offers valuable insights into its state. Detecting mRNA within single cells in real time and nondestructively remains an important challenge. Using DNA nanotechnology and inspired by nature’s many examples of “protective-yet-accessible” exoskeletons, we designed our mRNA nanosensor, nano-snail-inspired nucleic acid locator (nano-SNEL), to illustrate these elements. The design of the nano-SNEL is composed of a sensory molecular beacon module to detect mRNA and a DNA nanoshell component, mimicking the functional anatomy of a snail. Accurate and sensitive visualization of mRNA is achieved by the exceptional protection conferred by the nanoshell to the sensory component from nucleases-mediated degradation by approximately 9–25-fold compared to its unprotected counterpart. Our nano-SNEL design strategy improved cell internalization is a demonstration of accurate, dynamic spatiotemporal resolved detection of RNA transcripts in living cells. |
| doi_str_mv | 10.1021/acsnano.5b01954 |
| format | Article |
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Detecting mRNA within single cells in real time and nondestructively remains an important challenge. Using DNA nanotechnology and inspired by nature’s many examples of “protective-yet-accessible” exoskeletons, we designed our mRNA nanosensor, nano-snail-inspired nucleic acid locator (nano-SNEL), to illustrate these elements. The design of the nano-SNEL is composed of a sensory molecular beacon module to detect mRNA and a DNA nanoshell component, mimicking the functional anatomy of a snail. Accurate and sensitive visualization of mRNA is achieved by the exceptional protection conferred by the nanoshell to the sensory component from nucleases-mediated degradation by approximately 9–25-fold compared to its unprotected counterpart. Our nano-SNEL design strategy improved cell internalization is a demonstration of accurate, dynamic spatiotemporal resolved detection of RNA transcripts in living cells.</description><subject>Biological Transport</subject><subject>Biomimetics - instrumentation</subject><subject>Cell Line, Tumor</subject><subject>Cell Survival</subject><subject>DNA - chemistry</subject><subject>DNA - metabolism</subject><subject>Gene Expression Regulation, Enzymologic</subject><subject>Glyceraldehyde-3-Phosphate Dehydrogenases - genetics</subject><subject>Humans</subject><subject>Models, Molecular</subject><subject>Nanostructures - chemistry</subject><subject>Nanotechnology - instrumentation</subject><subject>Nucleic Acid Conformation</subject><subject>Nucleic Acid Hybridization</subject><subject>Oligonucleotide Probes - chemistry</subject><subject>RNA, Messenger - genetics</subject><subject>RNA, Messenger - metabolism</subject><subject>Time Factors</subject><issn>1936-0851</issn><issn>1936-086X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kF1LwzAUhoMoTqfX3knupVs-2ia9HJsfgzFhDvGupM2JZLTpSFrBf29kc3deHHIg7_NyeBC6o2RCCaNTVQenXDfJKkKLLD1DV7TgeUJk_nF-2jM6Qtch7AjJhBT5JRqxrCA5Z-IKva9VP3hIli7srQeNF-sZXsfKAC50Hps4G1BNsrUtYOvwm-0HvIAe6t52DncGt5uIxJ-V_bLuE8-hacINujCqCXB7fMdo-_S4nb8kq9fn5Xy2ShTnvE-0LGRhMgNGMSYqKTUYUacpEZRURmhdyExzozSrjeAVTyWttKS8VpQZEHyMpofa2ncheDDl3ttW-e-SkvJXUHkUVB4FReL-QOyHqgV9yv8ZiYGHQyCS5a4bvIv3_1v3A8LZcYA</recordid><startdate>20150526</startdate><enddate>20150526</enddate><creator>Tay, Chor Yong</creator><creator>Yuan, Liang</creator><creator>Leong, David Tai</creator><general>American Chemical Society</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>20150526</creationdate><title>Nature-Inspired DNA Nanosensor for Real-Time in Situ Detection of mRNA in Living Cells</title><author>Tay, Chor Yong ; Yuan, Liang ; Leong, David Tai</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a333t-d8989f5fefa227b88def7c440710bf7dd985d3fad2cf73b3481bd813ca12fe73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Biological Transport</topic><topic>Biomimetics - instrumentation</topic><topic>Cell Line, Tumor</topic><topic>Cell Survival</topic><topic>DNA - chemistry</topic><topic>DNA - metabolism</topic><topic>Gene Expression Regulation, Enzymologic</topic><topic>Glyceraldehyde-3-Phosphate Dehydrogenases - genetics</topic><topic>Humans</topic><topic>Models, Molecular</topic><topic>Nanostructures - chemistry</topic><topic>Nanotechnology - instrumentation</topic><topic>Nucleic Acid Conformation</topic><topic>Nucleic Acid Hybridization</topic><topic>Oligonucleotide Probes - chemistry</topic><topic>RNA, Messenger - genetics</topic><topic>RNA, Messenger - metabolism</topic><topic>Time Factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tay, Chor Yong</creatorcontrib><creatorcontrib>Yuan, Liang</creatorcontrib><creatorcontrib>Leong, David Tai</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><jtitle>ACS nano</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tay, Chor Yong</au><au>Yuan, Liang</au><au>Leong, David Tai</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Nature-Inspired DNA Nanosensor for Real-Time in Situ Detection of mRNA in Living Cells</atitle><jtitle>ACS nano</jtitle><addtitle>ACS Nano</addtitle><date>2015-05-26</date><risdate>2015</risdate><volume>9</volume><issue>5</issue><spage>5609</spage><epage>5617</epage><pages>5609-5617</pages><issn>1936-0851</issn><eissn>1936-086X</eissn><abstract>Rapid and precise in situ detection of gene expressions within a single cell is highly informative and offers valuable insights into its state. Detecting mRNA within single cells in real time and nondestructively remains an important challenge. Using DNA nanotechnology and inspired by nature’s many examples of “protective-yet-accessible” exoskeletons, we designed our mRNA nanosensor, nano-snail-inspired nucleic acid locator (nano-SNEL), to illustrate these elements. The design of the nano-SNEL is composed of a sensory molecular beacon module to detect mRNA and a DNA nanoshell component, mimicking the functional anatomy of a snail. Accurate and sensitive visualization of mRNA is achieved by the exceptional protection conferred by the nanoshell to the sensory component from nucleases-mediated degradation by approximately 9–25-fold compared to its unprotected counterpart. 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| language | eng |
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| source | MEDLINE; ACS Publications |
| subjects | Biological Transport Biomimetics - instrumentation Cell Line, Tumor Cell Survival DNA - chemistry DNA - metabolism Gene Expression Regulation, Enzymologic Glyceraldehyde-3-Phosphate Dehydrogenases - genetics Humans Models, Molecular Nanostructures - chemistry Nanotechnology - instrumentation Nucleic Acid Conformation Nucleic Acid Hybridization Oligonucleotide Probes - chemistry RNA, Messenger - genetics RNA, Messenger - metabolism Time Factors |
| title | Nature-Inspired DNA Nanosensor for Real-Time in Situ Detection of mRNA in Living Cells |
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