Intracellular FRET-based Screen for Redesigning the Specificity of Secreted Proteases

Proteases are attractive as therapeutics given their ability to catalytically activate or inactivate their targets. However, therapeutic use of proteases is limited by insufficient substrate specificity, since off-target activity can induce undesired side-effects. In addition, few methods exist to e...

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Veröffentlicht in:	ACS chemical biology 2016-04, Vol.11 (4), p.961-970
Hauptverfasser:	Guerrero, Jennifer L, O’Malley, Michelle A, Daugherty, Patrick S
Format:	Artikel
Sprache:	eng
Schlagworte:	Fluorescence Resonance Energy Transfer Peptide Hydrolases - metabolism Substrate Specificity
Online-Zugang:	Volltext
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