Dual-Peptide-Functionalized Nanofibrous Scaffolds Recruit Host Endothelial Progenitor Cells for Vasculogenesis to Repair Calvarial Defects
Vasculogenesis (de novo formation of vessels) induced by endothelial progenitor cells (EPCs) is requisite for vascularized bone regeneration. However, there exist few available options for promoting vasculogenesis within artificial bone grafts except for exogenous EPC transplantation, which suffers...
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| Veröffentlicht in: | ACS applied materials & interfaces 2020-01, Vol.12 (3), p.3474-3493 |
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| creator | Li, Li Liu, Wanqian Zhao, Yulan Ma, Pingping Zha, Shenfang Chen, Peixin Lu, Hongwei Jiang, Xiaorui Wan, Shuang Luo, Jiangming Dai, Qijie Hu, Junxian Utomo, Yohanes Kristo Sugiarto Han, Xinyun Yang, Zhengwei Yang, Li He, Qingyi |
| description | Vasculogenesis (de novo formation of vessels) induced by endothelial progenitor cells (EPCs) is requisite for vascularized bone regeneration. However, there exist few available options for promoting vasculogenesis within artificial bone grafts except for exogenous EPC transplantation, which suffers from the source of EPC, safety, cost, and time concerns in clinical applications. This study aimed at endogenous EPC recruitment for vascularized bone regeneration by using a bioinspired EPC-induced graft. The EPC-induced graft was created by immobilizing two bioactive peptides, WKYMVm and YIGSR, on the surface of poly(ε-caprolactone) (PCL)/poliglecaprone (PGC) nanofibrous scaffolds via a polyglycolic acid (PGA)-binding peptide sequence. Remarkable immobilization efficacy of WKYMVm and YIGSR peptides and their sustained release (over 14 days) from scaffolds were observed. In vivo and in vitro studies showed robust recruitment of EPCs, which subsequently contributed to early vasculogenesis and ultimate bone regeneration. The dual-peptide-functionalized nanofibrous scaffolds proposed in this study provide a promising therapeutic strategy for vasculogenesis in bone defect repair. |
| doi_str_mv | 10.1021/acsami.9b21434 |
| format | Article |
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However, there exist few available options for promoting vasculogenesis within artificial bone grafts except for exogenous EPC transplantation, which suffers from the source of EPC, safety, cost, and time concerns in clinical applications. This study aimed at endogenous EPC recruitment for vascularized bone regeneration by using a bioinspired EPC-induced graft. The EPC-induced graft was created by immobilizing two bioactive peptides, WKYMVm and YIGSR, on the surface of poly(ε-caprolactone) (PCL)/poliglecaprone (PGC) nanofibrous scaffolds via a polyglycolic acid (PGA)-binding peptide sequence. Remarkable immobilization efficacy of WKYMVm and YIGSR peptides and their sustained release (over 14 days) from scaffolds were observed. In vivo and in vitro studies showed robust recruitment of EPCs, which subsequently contributed to early vasculogenesis and ultimate bone regeneration. The dual-peptide-functionalized nanofibrous scaffolds proposed in this study provide a promising therapeutic strategy for vasculogenesis in bone defect repair.</description><identifier>ISSN: 1944-8244</identifier><identifier>EISSN: 1944-8252</identifier><identifier>DOI: 10.1021/acsami.9b21434</identifier><identifier>PMID: 31874023</identifier><language>eng</language><publisher>WASHINGTON: American Chemical Society</publisher><subject>Animals ; Bone Diseases - physiopathology ; Bone Diseases - therapy ; Bone Regeneration ; Cell Adhesion ; Cell Proliferation ; Endothelial Progenitor Cells - cytology ; Endothelial Progenitor Cells - transplantation ; Humans ; Male ; Materials Science ; Materials Science, Multidisciplinary ; Nanofibers - chemistry ; Nanoscience & Nanotechnology ; Neovascularization, Pathologic ; Peptides - administration & dosage ; Peptides - chemistry ; Rats ; Rats, Sprague-Dawley ; Science & Technology ; Science & Technology - Other Topics ; Skull - abnormalities ; Skull - blood supply ; Skull - surgery ; Technology ; Tissue Engineering ; Tissue Scaffolds - chemistry</subject><ispartof>ACS applied materials & interfaces, 2020-01, Vol.12 (3), p.3474-3493</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>true</woscitedreferencessubscribed><woscitedreferencescount>18</woscitedreferencescount><woscitedreferencesoriginalsourcerecordid>wos000509428300017</woscitedreferencesoriginalsourcerecordid><citedby>FETCH-LOGICAL-a330t-f73478cdc337adbbca81aea2330c6efd91249c110331b81f0a955a7c34c7a9383</citedby><cites>FETCH-LOGICAL-a330t-f73478cdc337adbbca81aea2330c6efd91249c110331b81f0a955a7c34c7a9383</cites><orcidid>0000-0001-7871-2698</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/acsami.9b21434$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/acsami.9b21434$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>315,781,785,2766,27081,27929,27930,28253,56743,56793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31874023$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Li, Li</creatorcontrib><creatorcontrib>Liu, Wanqian</creatorcontrib><creatorcontrib>Zhao, Yulan</creatorcontrib><creatorcontrib>Ma, Pingping</creatorcontrib><creatorcontrib>Zha, Shenfang</creatorcontrib><creatorcontrib>Chen, Peixin</creatorcontrib><creatorcontrib>Lu, Hongwei</creatorcontrib><creatorcontrib>Jiang, Xiaorui</creatorcontrib><creatorcontrib>Wan, Shuang</creatorcontrib><creatorcontrib>Luo, Jiangming</creatorcontrib><creatorcontrib>Dai, Qijie</creatorcontrib><creatorcontrib>Hu, Junxian</creatorcontrib><creatorcontrib>Utomo, Yohanes Kristo Sugiarto</creatorcontrib><creatorcontrib>Han, Xinyun</creatorcontrib><creatorcontrib>Yang, Zhengwei</creatorcontrib><creatorcontrib>Yang, Li</creatorcontrib><creatorcontrib>He, Qingyi</creatorcontrib><title>Dual-Peptide-Functionalized Nanofibrous Scaffolds Recruit Host Endothelial Progenitor Cells for Vasculogenesis to Repair Calvarial Defects</title><title>ACS applied materials & interfaces</title><addtitle>ACS APPL MATER INTER</addtitle><addtitle>ACS Appl. Mater. Interfaces</addtitle><description>Vasculogenesis (de novo formation of vessels) induced by endothelial progenitor cells (EPCs) is requisite for vascularized bone regeneration. However, there exist few available options for promoting vasculogenesis within artificial bone grafts except for exogenous EPC transplantation, which suffers from the source of EPC, safety, cost, and time concerns in clinical applications. This study aimed at endogenous EPC recruitment for vascularized bone regeneration by using a bioinspired EPC-induced graft. The EPC-induced graft was created by immobilizing two bioactive peptides, WKYMVm and YIGSR, on the surface of poly(ε-caprolactone) (PCL)/poliglecaprone (PGC) nanofibrous scaffolds via a polyglycolic acid (PGA)-binding peptide sequence. Remarkable immobilization efficacy of WKYMVm and YIGSR peptides and their sustained release (over 14 days) from scaffolds were observed. In vivo and in vitro studies showed robust recruitment of EPCs, which subsequently contributed to early vasculogenesis and ultimate bone regeneration. The dual-peptide-functionalized nanofibrous scaffolds proposed in this study provide a promising therapeutic strategy for vasculogenesis in bone defect repair.</description><subject>Animals</subject><subject>Bone Diseases - physiopathology</subject><subject>Bone Diseases - therapy</subject><subject>Bone Regeneration</subject><subject>Cell Adhesion</subject><subject>Cell Proliferation</subject><subject>Endothelial Progenitor Cells - cytology</subject><subject>Endothelial Progenitor Cells - transplantation</subject><subject>Humans</subject><subject>Male</subject><subject>Materials Science</subject><subject>Materials Science, Multidisciplinary</subject><subject>Nanofibers - chemistry</subject><subject>Nanoscience & Nanotechnology</subject><subject>Neovascularization, Pathologic</subject><subject>Peptides - administration & dosage</subject><subject>Peptides - chemistry</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Science & Technology</subject><subject>Science & Technology - Other Topics</subject><subject>Skull - abnormalities</subject><subject>Skull - blood supply</subject><subject>Skull - surgery</subject><subject>Technology</subject><subject>Tissue Engineering</subject><subject>Tissue Scaffolds - chemistry</subject><issn>1944-8244</issn><issn>1944-8252</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>AOWDO</sourceid><sourceid>EIF</sourceid><recordid>eNqNkUtP3DAURq0KVB7ttkvkNVUGv0KSJcrwqIQA9bWNbpxrMPLEI9uhKj-BX41HobOrxMqfdM-xrr5LyBfOFpwJfgI6wsouml5wJdUHss8bpYpalGJnm5XaIwcxPjJ2KgUrP5I9yetKMSH3yctyAlfc4TrZAYuLadTJ-hGcfcaB3sDoje2DnyL9ocEY74ZIv6MOk030ysdEz8fBpwd0Fhy9C_4eR5t8oC06F6nJ6TdEPbnNAKONNPnsr8FmBNwThI23RIM6xU9k14CL-PntPSS_Ls5_tlfF9e3lt_bsugApWSpMJVVV60FLWcHQ9xpqDggiD_UpmqHhQjWacyYl72tuGDRlCZWWSlfQyFoeksX8rw4-xoCmWwe7gvC346zblNrNpXZvpWbhaBbWU7_CYYv_azEDX2fgD_beRG1x1LjFGGMla5SoZU68ynT9frq1CTYXaf00pqwez2resHv0U8iXiv9b-xWZ5qWi</recordid><startdate>20200122</startdate><enddate>20200122</enddate><creator>Li, Li</creator><creator>Liu, Wanqian</creator><creator>Zhao, Yulan</creator><creator>Ma, Pingping</creator><creator>Zha, Shenfang</creator><creator>Chen, Peixin</creator><creator>Lu, Hongwei</creator><creator>Jiang, Xiaorui</creator><creator>Wan, Shuang</creator><creator>Luo, Jiangming</creator><creator>Dai, Qijie</creator><creator>Hu, Junxian</creator><creator>Utomo, Yohanes Kristo Sugiarto</creator><creator>Han, Xinyun</creator><creator>Yang, Zhengwei</creator><creator>Yang, Li</creator><creator>He, Qingyi</creator><general>American Chemical Society</general><general>Amer Chemical Soc</general><scope>AOWDO</scope><scope>BLEPL</scope><scope>DTL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><orcidid>https://orcid.org/0000-0001-7871-2698</orcidid></search><sort><creationdate>20200122</creationdate><title>Dual-Peptide-Functionalized Nanofibrous Scaffolds Recruit Host Endothelial Progenitor Cells for Vasculogenesis to Repair Calvarial Defects</title><author>Li, Li ; Liu, Wanqian ; Zhao, Yulan ; Ma, Pingping ; Zha, Shenfang ; Chen, Peixin ; Lu, Hongwei ; Jiang, Xiaorui ; Wan, Shuang ; Luo, Jiangming ; Dai, Qijie ; Hu, Junxian ; Utomo, Yohanes Kristo Sugiarto ; Han, Xinyun ; Yang, Zhengwei ; Yang, Li ; He, Qingyi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a330t-f73478cdc337adbbca81aea2330c6efd91249c110331b81f0a955a7c34c7a9383</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Animals</topic><topic>Bone Diseases - physiopathology</topic><topic>Bone Diseases - therapy</topic><topic>Bone Regeneration</topic><topic>Cell Adhesion</topic><topic>Cell Proliferation</topic><topic>Endothelial Progenitor Cells - cytology</topic><topic>Endothelial Progenitor Cells - transplantation</topic><topic>Humans</topic><topic>Male</topic><topic>Materials Science</topic><topic>Materials Science, Multidisciplinary</topic><topic>Nanofibers - chemistry</topic><topic>Nanoscience & Nanotechnology</topic><topic>Neovascularization, Pathologic</topic><topic>Peptides - administration & dosage</topic><topic>Peptides - chemistry</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Science & Technology</topic><topic>Science & Technology - Other Topics</topic><topic>Skull - abnormalities</topic><topic>Skull - blood supply</topic><topic>Skull - surgery</topic><topic>Technology</topic><topic>Tissue Engineering</topic><topic>Tissue Scaffolds - chemistry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Li, Li</creatorcontrib><creatorcontrib>Liu, Wanqian</creatorcontrib><creatorcontrib>Zhao, Yulan</creatorcontrib><creatorcontrib>Ma, Pingping</creatorcontrib><creatorcontrib>Zha, Shenfang</creatorcontrib><creatorcontrib>Chen, Peixin</creatorcontrib><creatorcontrib>Lu, Hongwei</creatorcontrib><creatorcontrib>Jiang, Xiaorui</creatorcontrib><creatorcontrib>Wan, Shuang</creatorcontrib><creatorcontrib>Luo, Jiangming</creatorcontrib><creatorcontrib>Dai, Qijie</creatorcontrib><creatorcontrib>Hu, Junxian</creatorcontrib><creatorcontrib>Utomo, Yohanes Kristo Sugiarto</creatorcontrib><creatorcontrib>Han, Xinyun</creatorcontrib><creatorcontrib>Yang, Zhengwei</creatorcontrib><creatorcontrib>Yang, Li</creatorcontrib><creatorcontrib>He, Qingyi</creatorcontrib><collection>Web of Science - Science Citation Index Expanded - 2020</collection><collection>Web of Science Core Collection</collection><collection>Science Citation Index Expanded</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><jtitle>ACS applied materials & interfaces</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Li, Li</au><au>Liu, Wanqian</au><au>Zhao, Yulan</au><au>Ma, Pingping</au><au>Zha, Shenfang</au><au>Chen, Peixin</au><au>Lu, Hongwei</au><au>Jiang, Xiaorui</au><au>Wan, Shuang</au><au>Luo, Jiangming</au><au>Dai, Qijie</au><au>Hu, Junxian</au><au>Utomo, Yohanes Kristo Sugiarto</au><au>Han, Xinyun</au><au>Yang, Zhengwei</au><au>Yang, Li</au><au>He, Qingyi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Dual-Peptide-Functionalized Nanofibrous Scaffolds Recruit Host Endothelial Progenitor Cells for Vasculogenesis to Repair Calvarial Defects</atitle><jtitle>ACS applied materials & interfaces</jtitle><stitle>ACS APPL MATER INTER</stitle><addtitle>ACS Appl. Mater. Interfaces</addtitle><date>2020-01-22</date><risdate>2020</risdate><volume>12</volume><issue>3</issue><spage>3474</spage><epage>3493</epage><pages>3474-3493</pages><issn>1944-8244</issn><eissn>1944-8252</eissn><abstract>Vasculogenesis (de novo formation of vessels) induced by endothelial progenitor cells (EPCs) is requisite for vascularized bone regeneration. However, there exist few available options for promoting vasculogenesis within artificial bone grafts except for exogenous EPC transplantation, which suffers from the source of EPC, safety, cost, and time concerns in clinical applications. This study aimed at endogenous EPC recruitment for vascularized bone regeneration by using a bioinspired EPC-induced graft. The EPC-induced graft was created by immobilizing two bioactive peptides, WKYMVm and YIGSR, on the surface of poly(ε-caprolactone) (PCL)/poliglecaprone (PGC) nanofibrous scaffolds via a polyglycolic acid (PGA)-binding peptide sequence. Remarkable immobilization efficacy of WKYMVm and YIGSR peptides and their sustained release (over 14 days) from scaffolds were observed. In vivo and in vitro studies showed robust recruitment of EPCs, which subsequently contributed to early vasculogenesis and ultimate bone regeneration. The dual-peptide-functionalized nanofibrous scaffolds proposed in this study provide a promising therapeutic strategy for vasculogenesis in bone defect repair.</abstract><cop>WASHINGTON</cop><pub>American Chemical Society</pub><pmid>31874023</pmid><doi>10.1021/acsami.9b21434</doi><tpages>20</tpages><orcidid>https://orcid.org/0000-0001-7871-2698</orcidid></addata></record> |
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| subjects | Animals Bone Diseases - physiopathology Bone Diseases - therapy Bone Regeneration Cell Adhesion Cell Proliferation Endothelial Progenitor Cells - cytology Endothelial Progenitor Cells - transplantation Humans Male Materials Science Materials Science, Multidisciplinary Nanofibers - chemistry Nanoscience & Nanotechnology Neovascularization, Pathologic Peptides - administration & dosage Peptides - chemistry Rats Rats, Sprague-Dawley Science & Technology Science & Technology - Other Topics Skull - abnormalities Skull - blood supply Skull - surgery Technology Tissue Engineering Tissue Scaffolds - chemistry |
| title | Dual-Peptide-Functionalized Nanofibrous Scaffolds Recruit Host Endothelial Progenitor Cells for Vasculogenesis to Repair Calvarial Defects |
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