Enhancing the Photocatalytic Conversion of Pt(IV) Substrates by Flavoprotein Engineering

Our recent work demonstrates that certain flavoproteins can catalyze the redox activation of Pt(IV) prodrug complexes under light irradiation. Herein, we used site-directed mutagenesis on the mini singlet oxygen generator (mSOG) to modulate the photocatalytic activity of this flavoprotein toward tw...

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Veröffentlicht in:	The journal of physical chemistry letters 2021-05, Vol.12 (19), p.4504-4508
Hauptverfasser:	Gurruchaga-Pereda, Juan, Martínez-Martínez, Virginia, Formoso, Elena, Azpitarte, Oksana, Rezabal, Elixabete, Lopez, Xabier, Cortajarena, Aitziber L, Salassa, Luca
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Sprache:	eng
Schlagworte:	Physical Insights into Chemistry, Catalysis, and Interfaces
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container_title	The journal of physical chemistry letters
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creator	Gurruchaga-Pereda, Juan Martínez-Martínez, Virginia Formoso, Elena Azpitarte, Oksana Rezabal, Elixabete Lopez, Xabier Cortajarena, Aitziber L Salassa, Luca
description	Our recent work demonstrates that certain flavoproteins can catalyze the redox activation of Pt(IV) prodrug complexes under light irradiation. Herein, we used site-directed mutagenesis on the mini singlet oxygen generator (mSOG) to modulate the photocatalytic activity of this flavoprotein toward two model Pt(IV) substrates. Among the prepared mutants, Q103V mSOG displayed enhanced catalytic efficiency as a result of its longer triplet excited-state lifetime. This study shows, for the first time, that protein engineering can improve the catalytic capacity of a protein toward metal-containing substrates.
doi_str_mv	10.1021/acs.jpclett.1c00802
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title	Enhancing the Photocatalytic Conversion of Pt(IV) Substrates by Flavoprotein Engineering
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