Modification of the pH Dependence of Assembly of Yeast Cargo Receptor Emp47p Coiled-Coil Domains: Computational Design and Experimental Mutagenesis

The coiled-coil domains of the putative yeast cargo receptors Emp46p and Emp47p (Emp46pcc and Emp47pcc) assemble into heterocomplexes at neutral pH. Upon lowering the pH, the complex dissociates and reassembles into homo-oligomers. A glutamate residue (E303) located on the hydrophobic surface of Emp...

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Veröffentlicht in:	The journal of physical chemistry. B 2021-03, Vol.125 (9), p.2222-2230
Hauptverfasser:	Kato, Koichi, Nakayoshi, Tomoki, Kurimoto, Eiji, Oda, Akifumi
Format:	Artikel
Sprache:	eng
Schlagworte:	B: Biophysical and Biochemical Systems and Processes Hydrogen-Ion Concentration Mutagenesis Protein Domains Saccharomyces cerevisiae Saccharomyces cerevisiae Proteins - genetics Saccharomyces cerevisiae Proteins - metabolism
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