Comparative Study of Nonautolytic Mutant and Wild-Type Strains of Coprinopsis cinerea Supports an Important Role of Glucanases in Fruiting Body Autolysis

Autolysis of Coprinopsis cinerea fruiting bodies affects its commercial value. In this study, a mutant of C. cinerea that exhibits pileus expansion without pileus autolysis was obtained using ultraviolet mutagenesis. This suggests that pileus expansion and pileus autolysis involve different enzymes...

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Veröffentlicht in:Journal of agricultural and food chemistry 2015-11, Vol.63 (43), p.9609-9614
Hauptverfasser: Liu, Zhonghua, Niu, Xin, Wang, Jun, Zhang, Wenming, Yang, Mingmei, Liu, Cuicui, Xiong, Yuanjing, Zhao, Yan, Pei, Siyu, Qin, Qin, Zhang, Yu, Yu, Yuan, Yuan, Sheng
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container_end_page 9614
container_issue 43
container_start_page 9609
container_title Journal of agricultural and food chemistry
container_volume 63
creator Liu, Zhonghua
Niu, Xin
Wang, Jun
Zhang, Wenming
Yang, Mingmei
Liu, Cuicui
Xiong, Yuanjing
Zhao, Yan
Pei, Siyu
Qin, Qin
Zhang, Yu
Yu, Yuan
Yuan, Sheng
description Autolysis of Coprinopsis cinerea fruiting bodies affects its commercial value. In this study, a mutant of C. cinerea that exhibits pileus expansion without pileus autolysis was obtained using ultraviolet mutagenesis. This suggests that pileus expansion and pileus autolysis involve different enzymes or proteins. Among the detected hydrolytic enzymes, only β-1,3-glucanase activity increased with expansion and autolysis of pilei in the wild-type strain, but the increase was abolished in the mutant. This suggests that β-1,3-glucanases plays a major role in the autolysis. Although there are 43 possible β-1,3-glucoside hydrolases genes, only 4 known genes, which have products that are thought to act synergistically to degrade the β-1,3-glucan backbone of cell walls during fruiting body autolysis, and an unreported gene were upregulated during pileus expansion and autolysis in the wild-type stain but were suppressed in the mutant. This suggests that expression of these β-1,3-glucanases is potentially controlled by a single regulatory mechanism.
doi_str_mv 10.1021/acs.jafc.5b03962
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In this study, a mutant of C. cinerea that exhibits pileus expansion without pileus autolysis was obtained using ultraviolet mutagenesis. This suggests that pileus expansion and pileus autolysis involve different enzymes or proteins. Among the detected hydrolytic enzymes, only β-1,3-glucanase activity increased with expansion and autolysis of pilei in the wild-type strain, but the increase was abolished in the mutant. This suggests that β-1,3-glucanases plays a major role in the autolysis. Although there are 43 possible β-1,3-glucoside hydrolases genes, only 4 known genes, which have products that are thought to act synergistically to degrade the β-1,3-glucan backbone of cell walls during fruiting body autolysis, and an unreported gene were upregulated during pileus expansion and autolysis in the wild-type stain but were suppressed in the mutant. 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Agric. Food Chem</addtitle><description>Autolysis of Coprinopsis cinerea fruiting bodies affects its commercial value. In this study, a mutant of C. cinerea that exhibits pileus expansion without pileus autolysis was obtained using ultraviolet mutagenesis. This suggests that pileus expansion and pileus autolysis involve different enzymes or proteins. Among the detected hydrolytic enzymes, only β-1,3-glucanase activity increased with expansion and autolysis of pilei in the wild-type strain, but the increase was abolished in the mutant. This suggests that β-1,3-glucanases plays a major role in the autolysis. Although there are 43 possible β-1,3-glucoside hydrolases genes, only 4 known genes, which have products that are thought to act synergistically to degrade the β-1,3-glucan backbone of cell walls during fruiting body autolysis, and an unreported gene were upregulated during pileus expansion and autolysis in the wild-type stain but were suppressed in the mutant. 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subjects Agaricales - enzymology
Agaricales - genetics
Agaricales - growth & development
Agaricales - physiology
Autolysis
Fruiting Bodies, Fungal - enzymology
Fruiting Bodies, Fungal - genetics
Fruiting Bodies, Fungal - growth & development
Fruiting Bodies, Fungal - physiology
Fungal Proteins - genetics
Fungal Proteins - metabolism
Gene Expression Regulation, Fungal
Glycoside Hydrolases - genetics
Glycoside Hydrolases - metabolism
Mutation
title Comparative Study of Nonautolytic Mutant and Wild-Type Strains of Coprinopsis cinerea Supports an Important Role of Glucanases in Fruiting Body Autolysis
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