Methylation of MYBA1 is Associated with the Coloration in “Manicure Finger” Grape Skin
The “Manicure Finger” grape is notable for its fingerlike berries with a bright red top and yellow base; however, the mechanism underlying this color difference remains unknown. This study showed that the anthocyanin concentration and the expression levels of anthocyanin-related genes in the top ski...
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| Veröffentlicht in: | Journal of agricultural and food chemistry 2021-12, Vol.69 (51), p.15649-15659 |
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| container_issue | 51 |
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| container_title | Journal of agricultural and food chemistry |
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| creator | Xia, Hui Shen, Yanqiu Hu, Rongping Wang, Jin Deng, Honghong Lin, Lijin Lv, Xiulan Deng, Qunxian Xu, Kunfu Liang, Dong |
| description | The “Manicure Finger” grape is notable for its fingerlike berries with a bright red top and yellow base; however, the mechanism underlying this color difference remains unknown. This study showed that the anthocyanin concentration and the expression levels of anthocyanin-related genes in the top skin were notably higher than those in the basal skin. The expression levels of DFR, UFGT, and GST were significantly correlated with the anthocyanin content. The promoters of the two VvUFGT alleles can be activated by VvMYBA1, which was verified by the yeast one-hybrid assay, the dual-luciferase reporter gene assay, and the electrophoretic mobility shift assay. Moreover, the methylation level of the VvMYBA1 promoter (−1488 to −1083 bp) in the top skin was significantly lower than that in the basal skin and was positively correlated with the anthocyanin content. Our data suggest that methylation levels of the VvMYBA1 promoter play a crucial role in regulating grape skin coloration. |
| doi_str_mv | 10.1021/acs.jafc.1c04550 |
| format | Article |
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This study showed that the anthocyanin concentration and the expression levels of anthocyanin-related genes in the top skin were notably higher than those in the basal skin. The expression levels of DFR, UFGT, and GST were significantly correlated with the anthocyanin content. The promoters of the two VvUFGT alleles can be activated by VvMYBA1, which was verified by the yeast one-hybrid assay, the dual-luciferase reporter gene assay, and the electrophoretic mobility shift assay. Moreover, the methylation level of the VvMYBA1 promoter (−1488 to −1083 bp) in the top skin was significantly lower than that in the basal skin and was positively correlated with the anthocyanin content. Our data suggest that methylation levels of the VvMYBA1 promoter play a crucial role in regulating grape skin coloration.</description><identifier>ISSN: 0021-8561</identifier><identifier>EISSN: 1520-5118</identifier><identifier>DOI: 10.1021/acs.jafc.1c04550</identifier><identifier>PMID: 34918911</identifier><language>eng</language><publisher>WASHINGTON: American Chemical Society</publisher><subject>Agriculture ; Agriculture, Multidisciplinary ; Anthocyanins - metabolism ; Biotechnology and Biological Transformations ; Chemistry ; Chemistry, Applied ; Food Science & Technology ; Fruit - genetics ; Fruit - metabolism ; Gene Expression Regulation, Plant ; Life Sciences & Biomedicine ; Methylation ; Physical Sciences ; Plant Proteins - genetics ; Plant Proteins - metabolism ; Science & Technology ; Vitis - genetics ; Vitis - metabolism</subject><ispartof>Journal of agricultural and food chemistry, 2021-12, Vol.69 (51), p.15649-15659</ispartof><rights>2021 American Chemical Society</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>true</woscitedreferencessubscribed><woscitedreferencescount>14</woscitedreferencescount><woscitedreferencesoriginalsourcerecordid>wos000734625200001</woscitedreferencesoriginalsourcerecordid><citedby>FETCH-LOGICAL-a336t-c4f95322aded27c42483ea1e5030b2051975124d0c4c774bc3cb4ed3a0a524f03</citedby><cites>FETCH-LOGICAL-a336t-c4f95322aded27c42483ea1e5030b2051975124d0c4c774bc3cb4ed3a0a524f03</cites><orcidid>0000-0002-0551-6933 ; 0000-0003-0495-4744</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/acs.jafc.1c04550$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/acs.jafc.1c04550$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>315,781,785,2766,27080,27928,27929,56742,56792</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/34918911$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Xia, Hui</creatorcontrib><creatorcontrib>Shen, Yanqiu</creatorcontrib><creatorcontrib>Hu, Rongping</creatorcontrib><creatorcontrib>Wang, Jin</creatorcontrib><creatorcontrib>Deng, Honghong</creatorcontrib><creatorcontrib>Lin, Lijin</creatorcontrib><creatorcontrib>Lv, Xiulan</creatorcontrib><creatorcontrib>Deng, Qunxian</creatorcontrib><creatorcontrib>Xu, Kunfu</creatorcontrib><creatorcontrib>Liang, Dong</creatorcontrib><title>Methylation of MYBA1 is Associated with the Coloration in “Manicure Finger” Grape Skin</title><title>Journal of agricultural and food chemistry</title><addtitle>J AGR FOOD CHEM</addtitle><addtitle>J. Agric. Food Chem</addtitle><description>The “Manicure Finger” grape is notable for its fingerlike berries with a bright red top and yellow base; however, the mechanism underlying this color difference remains unknown. This study showed that the anthocyanin concentration and the expression levels of anthocyanin-related genes in the top skin were notably higher than those in the basal skin. The expression levels of DFR, UFGT, and GST were significantly correlated with the anthocyanin content. The promoters of the two VvUFGT alleles can be activated by VvMYBA1, which was verified by the yeast one-hybrid assay, the dual-luciferase reporter gene assay, and the electrophoretic mobility shift assay. Moreover, the methylation level of the VvMYBA1 promoter (−1488 to −1083 bp) in the top skin was significantly lower than that in the basal skin and was positively correlated with the anthocyanin content. Our data suggest that methylation levels of the VvMYBA1 promoter play a crucial role in regulating grape skin coloration.</description><subject>Agriculture</subject><subject>Agriculture, Multidisciplinary</subject><subject>Anthocyanins - metabolism</subject><subject>Biotechnology and Biological Transformations</subject><subject>Chemistry</subject><subject>Chemistry, Applied</subject><subject>Food Science & Technology</subject><subject>Fruit - genetics</subject><subject>Fruit - metabolism</subject><subject>Gene Expression Regulation, Plant</subject><subject>Life Sciences & Biomedicine</subject><subject>Methylation</subject><subject>Physical Sciences</subject><subject>Plant Proteins - genetics</subject><subject>Plant Proteins - metabolism</subject><subject>Science & Technology</subject><subject>Vitis - genetics</subject><subject>Vitis - metabolism</subject><issn>0021-8561</issn><issn>1520-5118</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>HGBXW</sourceid><sourceid>EIF</sourceid><recordid>eNqNkU1v1DAQhi0Eokvhzgn5iARZPP5IssclogWpKw7AAS6R40xYl6y92I6q3vpD4M_1l-Btlt6QmMtcnvfV6BlCngNbAuPwRpu4vNSDWYJhUin2gCxAcVYogPohWbDMFLUq4YQ8ifGSMVarij0mJ0KuoF4BLMi3Dabt9aiT9Y76gW6-vl0DtZGuY_TG6oQ9vbJpS9MWaeNHH2bUOnp782ujnTVTQHpm3XcMtze_6XnQe6Sfflj3lDwa9Bjx2XGfki9n7z4374uLj-cfmvVFoYUoU2HksFKCc91jzysjuawFakDFBOs4U7CqFHDZMyNNVcnOCNNJ7IVmWnE5MHFKXs69--B_ThhTu7PR4Dhqh36KLS8BSgU1HFA2oyb4GAMO7T7YnQ7XLbD2YLTNRtuD0fZoNEdeHNunbof9feCvwgzUM3CFnR-isegM3mPZeSVkyfNX8kBj052_xk8u5eir_49m-vVM393op-Cy1X8f_gfK3qLb</recordid><startdate>20211229</startdate><enddate>20211229</enddate><creator>Xia, Hui</creator><creator>Shen, Yanqiu</creator><creator>Hu, Rongping</creator><creator>Wang, Jin</creator><creator>Deng, Honghong</creator><creator>Lin, Lijin</creator><creator>Lv, Xiulan</creator><creator>Deng, Qunxian</creator><creator>Xu, Kunfu</creator><creator>Liang, Dong</creator><general>American Chemical Society</general><general>Amer Chemical Soc</general><scope>BLEPL</scope><scope>DTL</scope><scope>HGBXW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-0551-6933</orcidid><orcidid>https://orcid.org/0000-0003-0495-4744</orcidid></search><sort><creationdate>20211229</creationdate><title>Methylation of MYBA1 is Associated with the Coloration in “Manicure Finger” Grape Skin</title><author>Xia, Hui ; Shen, Yanqiu ; Hu, Rongping ; Wang, Jin ; Deng, Honghong ; Lin, Lijin ; Lv, Xiulan ; Deng, Qunxian ; Xu, Kunfu ; Liang, Dong</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a336t-c4f95322aded27c42483ea1e5030b2051975124d0c4c774bc3cb4ed3a0a524f03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Agriculture</topic><topic>Agriculture, Multidisciplinary</topic><topic>Anthocyanins - metabolism</topic><topic>Biotechnology and Biological Transformations</topic><topic>Chemistry</topic><topic>Chemistry, Applied</topic><topic>Food Science & Technology</topic><topic>Fruit - genetics</topic><topic>Fruit - metabolism</topic><topic>Gene Expression Regulation, Plant</topic><topic>Life Sciences & Biomedicine</topic><topic>Methylation</topic><topic>Physical Sciences</topic><topic>Plant Proteins - genetics</topic><topic>Plant Proteins - metabolism</topic><topic>Science & Technology</topic><topic>Vitis - genetics</topic><topic>Vitis - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Xia, Hui</creatorcontrib><creatorcontrib>Shen, Yanqiu</creatorcontrib><creatorcontrib>Hu, Rongping</creatorcontrib><creatorcontrib>Wang, Jin</creatorcontrib><creatorcontrib>Deng, Honghong</creatorcontrib><creatorcontrib>Lin, Lijin</creatorcontrib><creatorcontrib>Lv, Xiulan</creatorcontrib><creatorcontrib>Deng, Qunxian</creatorcontrib><creatorcontrib>Xu, Kunfu</creatorcontrib><creatorcontrib>Liang, Dong</creatorcontrib><collection>Web of Science Core Collection</collection><collection>Science Citation Index Expanded</collection><collection>Web of Science - Science Citation Index Expanded - 2021</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of agricultural and food chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Xia, Hui</au><au>Shen, Yanqiu</au><au>Hu, Rongping</au><au>Wang, Jin</au><au>Deng, Honghong</au><au>Lin, Lijin</au><au>Lv, Xiulan</au><au>Deng, Qunxian</au><au>Xu, Kunfu</au><au>Liang, Dong</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Methylation of MYBA1 is Associated with the Coloration in “Manicure Finger” Grape Skin</atitle><jtitle>Journal of agricultural and food chemistry</jtitle><stitle>J AGR FOOD CHEM</stitle><addtitle>J. Agric. Food Chem</addtitle><date>2021-12-29</date><risdate>2021</risdate><volume>69</volume><issue>51</issue><spage>15649</spage><epage>15659</epage><pages>15649-15659</pages><issn>0021-8561</issn><eissn>1520-5118</eissn><abstract>The “Manicure Finger” grape is notable for its fingerlike berries with a bright red top and yellow base; however, the mechanism underlying this color difference remains unknown. This study showed that the anthocyanin concentration and the expression levels of anthocyanin-related genes in the top skin were notably higher than those in the basal skin. The expression levels of DFR, UFGT, and GST were significantly correlated with the anthocyanin content. The promoters of the two VvUFGT alleles can be activated by VvMYBA1, which was verified by the yeast one-hybrid assay, the dual-luciferase reporter gene assay, and the electrophoretic mobility shift assay. 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| subjects | Agriculture Agriculture, Multidisciplinary Anthocyanins - metabolism Biotechnology and Biological Transformations Chemistry Chemistry, Applied Food Science & Technology Fruit - genetics Fruit - metabolism Gene Expression Regulation, Plant Life Sciences & Biomedicine Methylation Physical Sciences Plant Proteins - genetics Plant Proteins - metabolism Science & Technology Vitis - genetics Vitis - metabolism |
| title | Methylation of MYBA1 is Associated with the Coloration in “Manicure Finger” Grape Skin |
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