Engineering of the Long-Main-Chain Monomer-Incorporating Polyhydroxyalkanoate Synthase PhaC AR for the Biosynthesis of Poly[( R )-3-hydroxybutyrate- co -6-hydroxyhexanoate]

Polyhydroxyalkanoate (PHA) synthases (PhaCs) are useful and versatile tools for the production of aliphatic polyesters. Here, the chimeric PHA synthase PhaC was engineered to increase its capacity to incorporate unusual 6-hydroxyhexanoate (6HHx) units. Mutations at positions 149 and 314 in PhaC were...

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Veröffentlicht in:	Biomacromolecules 2024-05, Vol.25 (5), p.2973-2979
Hauptverfasser:	Hozumi, Yuka, Hachisuka, Shin-Ichi, Tomita, Hiroya, Kikukawa, Hiroshi, Matsumoto, Ken'ichiro
Format:	Artikel
Sprache:	eng
Schlagworte:	Acyltransferases - genetics Acyltransferases - metabolism Bacterial Proteins - chemistry Bacterial Proteins - genetics Bacterial Proteins - metabolism Caproates - chemistry Caproates - metabolism Escherichia coli - genetics Escherichia coli - metabolism Mutagenesis, Site-Directed Polyesters - chemistry Polyesters - metabolism Polyhydroxyalkanoates - biosynthesis Polyhydroxyalkanoates - chemistry Protein Engineering - methods
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