Site-Specific 19 F NMR Method for Detecting Arf6 GEF Activity

Site-Specific 19 F NMR Method for Detecting Arf6 GEF Activity

Guanine nucleotide exchange factors (GEFs) of small GTPase (sGTPase) coordinate signal networks in normal cells and dysfunction in cancer. Therefore, effective monitoring of GEF activity is very important for studying the regulation of sGTPase signal transduction. In this study, we developed a 1D F...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Analytical chemistry (Washington) 2022-06, Vol.94 (23), p.8181-8186
Hauptverfasser: Wang, Jiarong, Wu, Bo, Zhang, Youjia, Ge, Liang, Wang, Junfeng
Format: Artikel
Sprache:eng
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page 8186
container_issue 23
container_start_page 8181
container_title Analytical chemistry (Washington)
container_volume 94
creator Wang, Jiarong
Wu, Bo
Zhang, Youjia
Ge, Liang
Wang, Junfeng
description Guanine nucleotide exchange factors (GEFs) of small GTPase (sGTPase) coordinate signal networks in normal cells and dysfunction in cancer. Therefore, effective monitoring of GEF activity is very important for studying the regulation of sGTPase signal transduction. In this study, we developed a 1D F NMR-based method for rapid detection of the GEF activity of sGTPases. The activity of Arf6GEF in vitro and cell lysate environment can be conveniently detected by tracking the conformational changes of the Arf6 switch region where a tfmF site-specific F labeling at Phe47 was introduced. This strategy could potentially be applied to monitor the conformational change of Arf6 or other sGTPase and detect the activities of sGTPase regulatory proteins in physiology and pathology environments.
doi_str_mv 10.1021/acs.analchem.1c05563
format Article
fullrecord <record><control><sourceid>pubmed_cross</sourceid><recordid>TN_cdi_crossref_primary_10_1021_acs_analchem_1c05563</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>35658403</sourcerecordid><originalsourceid>FETCH-LOGICAL-c1193-4552292146d776696aaa16bc5bec7110ca6c72bb1ceb7f124f565e56e37c5d853</originalsourceid><addsrcrecordid>eNo9kN1Kw0AQhRdRbK2-gci-QOLMbnY3ufCi1LYKrYLV67CZzNpI_0ii0Lc30tarw1x8wzmfELcIMYLCe09N7Dd-RUtex0hgjNVnoo9GQWTTVJ2LPgDoSDmAnrhqmi8AREB7KXraWJMmoPviYVG1HC12TFWoSGImJ_Jl_ibn3C63pQzbWj5yy9RWm085rIOV0_FEDrv7p2r31-Ii-FXDN8cciI_J-H30FM1ep8-j4SwixExHiTFKZQoTWzpnbWa992gLMgWT6zqRt-RUUSBx4QKqJHT92FjWjkyZGj0QyeEv1dumqTnku7pa-3qfI-R_NvLORn6ykR9tdNjdAdt9F2su_6HTfP0LU51bjg</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>Site-Specific 19 F NMR Method for Detecting Arf6 GEF Activity</title><source>American Chemical Society Journals</source><creator>Wang, Jiarong ; Wu, Bo ; Zhang, Youjia ; Ge, Liang ; Wang, Junfeng</creator><creatorcontrib>Wang, Jiarong ; Wu, Bo ; Zhang, Youjia ; Ge, Liang ; Wang, Junfeng</creatorcontrib><description>Guanine nucleotide exchange factors (GEFs) of small GTPase (sGTPase) coordinate signal networks in normal cells and dysfunction in cancer. Therefore, effective monitoring of GEF activity is very important for studying the regulation of sGTPase signal transduction. In this study, we developed a 1D F NMR-based method for rapid detection of the GEF activity of sGTPases. The activity of Arf6GEF in vitro and cell lysate environment can be conveniently detected by tracking the conformational changes of the Arf6 switch region where a tfmF site-specific F labeling at Phe47 was introduced. This strategy could potentially be applied to monitor the conformational change of Arf6 or other sGTPase and detect the activities of sGTPase regulatory proteins in physiology and pathology environments.</description><identifier>ISSN: 0003-2700</identifier><identifier>EISSN: 1520-6882</identifier><identifier>DOI: 10.1021/acs.analchem.1c05563</identifier><identifier>PMID: 35658403</identifier><language>eng</language><publisher>United States</publisher><ispartof>Analytical chemistry (Washington), 2022-06, Vol.94 (23), p.8181-8186</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c1193-4552292146d776696aaa16bc5bec7110ca6c72bb1ceb7f124f565e56e37c5d853</citedby><cites>FETCH-LOGICAL-c1193-4552292146d776696aaa16bc5bec7110ca6c72bb1ceb7f124f565e56e37c5d853</cites><orcidid>0000-0001-9152-5408 ; 0000-0002-3592-1454 ; 0000-0002-9608-6851</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,2765,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/35658403$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wang, Jiarong</creatorcontrib><creatorcontrib>Wu, Bo</creatorcontrib><creatorcontrib>Zhang, Youjia</creatorcontrib><creatorcontrib>Ge, Liang</creatorcontrib><creatorcontrib>Wang, Junfeng</creatorcontrib><title>Site-Specific 19 F NMR Method for Detecting Arf6 GEF Activity</title><title>Analytical chemistry (Washington)</title><addtitle>Anal Chem</addtitle><description>Guanine nucleotide exchange factors (GEFs) of small GTPase (sGTPase) coordinate signal networks in normal cells and dysfunction in cancer. Therefore, effective monitoring of GEF activity is very important for studying the regulation of sGTPase signal transduction. In this study, we developed a 1D F NMR-based method for rapid detection of the GEF activity of sGTPases. The activity of Arf6GEF in vitro and cell lysate environment can be conveniently detected by tracking the conformational changes of the Arf6 switch region where a tfmF site-specific F labeling at Phe47 was introduced. This strategy could potentially be applied to monitor the conformational change of Arf6 or other sGTPase and detect the activities of sGTPase regulatory proteins in physiology and pathology environments.</description><issn>0003-2700</issn><issn>1520-6882</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><recordid>eNo9kN1Kw0AQhRdRbK2-gci-QOLMbnY3ufCi1LYKrYLV67CZzNpI_0ii0Lc30tarw1x8wzmfELcIMYLCe09N7Dd-RUtex0hgjNVnoo9GQWTTVJ2LPgDoSDmAnrhqmi8AREB7KXraWJMmoPviYVG1HC12TFWoSGImJ_Jl_ibn3C63pQzbWj5yy9RWm085rIOV0_FEDrv7p2r31-Ii-FXDN8cciI_J-H30FM1ep8-j4SwixExHiTFKZQoTWzpnbWa992gLMgWT6zqRt-RUUSBx4QKqJHT92FjWjkyZGj0QyeEv1dumqTnku7pa-3qfI-R_NvLORn6ykR9tdNjdAdt9F2su_6HTfP0LU51bjg</recordid><startdate>20220614</startdate><enddate>20220614</enddate><creator>Wang, Jiarong</creator><creator>Wu, Bo</creator><creator>Zhang, Youjia</creator><creator>Ge, Liang</creator><creator>Wang, Junfeng</creator><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><orcidid>https://orcid.org/0000-0001-9152-5408</orcidid><orcidid>https://orcid.org/0000-0002-3592-1454</orcidid><orcidid>https://orcid.org/0000-0002-9608-6851</orcidid></search><sort><creationdate>20220614</creationdate><title>Site-Specific 19 F NMR Method for Detecting Arf6 GEF Activity</title><author>Wang, Jiarong ; Wu, Bo ; Zhang, Youjia ; Ge, Liang ; Wang, Junfeng</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c1193-4552292146d776696aaa16bc5bec7110ca6c72bb1ceb7f124f565e56e37c5d853</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wang, Jiarong</creatorcontrib><creatorcontrib>Wu, Bo</creatorcontrib><creatorcontrib>Zhang, Youjia</creatorcontrib><creatorcontrib>Ge, Liang</creatorcontrib><creatorcontrib>Wang, Junfeng</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><jtitle>Analytical chemistry (Washington)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wang, Jiarong</au><au>Wu, Bo</au><au>Zhang, Youjia</au><au>Ge, Liang</au><au>Wang, Junfeng</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Site-Specific 19 F NMR Method for Detecting Arf6 GEF Activity</atitle><jtitle>Analytical chemistry (Washington)</jtitle><addtitle>Anal Chem</addtitle><date>2022-06-14</date><risdate>2022</risdate><volume>94</volume><issue>23</issue><spage>8181</spage><epage>8186</epage><pages>8181-8186</pages><issn>0003-2700</issn><eissn>1520-6882</eissn><abstract>Guanine nucleotide exchange factors (GEFs) of small GTPase (sGTPase) coordinate signal networks in normal cells and dysfunction in cancer. Therefore, effective monitoring of GEF activity is very important for studying the regulation of sGTPase signal transduction. In this study, we developed a 1D F NMR-based method for rapid detection of the GEF activity of sGTPases. The activity of Arf6GEF in vitro and cell lysate environment can be conveniently detected by tracking the conformational changes of the Arf6 switch region where a tfmF site-specific F labeling at Phe47 was introduced. This strategy could potentially be applied to monitor the conformational change of Arf6 or other sGTPase and detect the activities of sGTPase regulatory proteins in physiology and pathology environments.</abstract><cop>United States</cop><pmid>35658403</pmid><doi>10.1021/acs.analchem.1c05563</doi><tpages>6</tpages><orcidid>https://orcid.org/0000-0001-9152-5408</orcidid><orcidid>https://orcid.org/0000-0002-3592-1454</orcidid><orcidid>https://orcid.org/0000-0002-9608-6851</orcidid></addata></record>
fulltext fulltext
identifier ISSN: 0003-2700
ispartof Analytical chemistry (Washington), 2022-06, Vol.94 (23), p.8181-8186
issn 0003-2700
1520-6882
language eng
recordid cdi_crossref_primary_10_1021_acs_analchem_1c05563
source American Chemical Society Journals
title Site-Specific 19 F NMR Method for Detecting Arf6 GEF Activity
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-27T14%3A12%3A45IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-pubmed_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Site-Specific%2019%20F%20NMR%20Method%20for%20Detecting%20Arf6%20GEF%20Activity&rft.jtitle=Analytical%20chemistry%20(Washington)&rft.au=Wang,%20Jiarong&rft.date=2022-06-14&rft.volume=94&rft.issue=23&rft.spage=8181&rft.epage=8186&rft.pages=8181-8186&rft.issn=0003-2700&rft.eissn=1520-6882&rft_id=info:doi/10.1021/acs.analchem.1c05563&rft_dat=%3Cpubmed_cross%3E35658403%3C/pubmed_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/35658403&rfr_iscdi=true