Enzymatic Spectrophotometric Method for Aflatoxin B Detection Based on Acetylcholinesterase Inhibition

A new method for aflatoxin B (AFB) determination is proposed. The AFB determination is based on acetylcholinesterase (AChE) inhibition, and the AChE residual activity is determined using the colorimetric method (Ellman's method). Cholinesterases (ChEs) from various sources were tested using AFB...

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Veröffentlicht in:	Analytical chemistry (Washington) 2007-05, Vol.79 (9), p.3409-3415
Hauptverfasser:	Arduini, Fabiana, Errico, Ilenia, Amine, Aziz, Micheli, Laura, Palleschi, Giuseppe, Moscone, Danila
Format:	Artikel
Sprache:	eng
Schlagworte:	Acetylcholinesterase - chemistry Acetylcholinesterase - drug effects Aflatoxin B1 - analysis Aflatoxin B1 - pharmacology Analytical chemistry Animals Chemistry Electrophorus Enzyme Activation - drug effects Exact sciences and technology Methanol - chemistry Methanol - pharmacology Molecular Structure Sensitivity and Specificity Spectrometric and optical methods Spectrophotometry - methods Structure-Activity Relationship Temperature
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