Enzymatic Spectrophotometric Method for Aflatoxin B Detection Based on Acetylcholinesterase Inhibition

A new method for aflatoxin B (AFB) determination is proposed. The AFB determination is based on acetylcholinesterase (AChE) inhibition, and the AChE residual activity is determined using the colorimetric method (Ellman's method). Cholinesterases (ChEs) from various sources were tested using AFB...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Analytical chemistry (Washington) 2007-05, Vol.79 (9), p.3409-3415
Hauptverfasser: Arduini, Fabiana, Errico, Ilenia, Amine, Aziz, Micheli, Laura, Palleschi, Giuseppe, Moscone, Danila
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page 3415
container_issue 9
container_start_page 3409
container_title Analytical chemistry (Washington)
container_volume 79
creator Arduini, Fabiana
Errico, Ilenia
Amine, Aziz
Micheli, Laura
Palleschi, Giuseppe
Moscone, Danila
description A new method for aflatoxin B (AFB) determination is proposed. The AFB determination is based on acetylcholinesterase (AChE) inhibition, and the AChE residual activity is determined using the colorimetric method (Ellman's method). Cholinesterases (ChEs) from various sources were tested using AFB1 as reference aflatoxin. AChE from electric eel has shown the highest sensitivity to AFB1, and it was chosen for the rest of the work. To select and optimize the analytical procedures, an investigation on the type of AChE inhibition by AFB1 was carried out. The AChE degree of inhibition by AFB1 was independent of the incubation time and the enzyme concentrations, showing the reversibility of the inhibition. This reversibility of the inhibition permits a rapid analysis of AFB1, requiring only 3 min. For the development of the AFB1 assay, the pH, the time of reaction, temperature, and substrate concentration were evaluated and optimized. The linear range of 10−60 ng mL-1 was determined. To evaluate the selectivity of this method, the cross-reactivity with other aflatoxins such as aflatoxin B2, aflatoxin G1, aflatoxin G2, and aflatoxin M1 was investigated. Finally, the suitability of the assay for AFB1 quantification in barley was evaluated. This study shows a new approach to detect aflatoxins based on enzyme inhibition and has advantages such as the ease of use, rapidity, and cost effectiveness. Thus, it could find a possible use as a screening method for this type of mycotoxins.
doi_str_mv 10.1021/ac061819j
format Article
fullrecord <record><control><sourceid>istex_cross</sourceid><recordid>TN_cdi_crossref_primary_10_1021_ac061819j</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>ark_67375_TPS_G4CLXV0W_G</sourcerecordid><originalsourceid>FETCH-LOGICAL-a447t-ba9bcb0af800388ceba53a0643ad05b869ad1e6603f08199f951e84b1f8c79cd3</originalsourceid><addsrcrecordid>eNpt0EtLAzEQB_AgitbHwS8ge_HgYXWyj2z2WKtWoaLg8xZmswlN3W5KEsH66Y202IunDJkfw8yfkGMK5xQyeoESGOW0nm2RAS0zSBnn2TYZAECeZhXAHtn3fgZAKVC2S_ZoVQDPimxA9HX_vZxjMDJ5WigZnF1MbbBzFVz8uldhattEW5cMdYfBfpk-uUyuVIjU2FijV20Si6FUYdnJqe1Mr3xQLjaSu35qGvMLD8mOxs6ro_V7QF5urp9Ht-nkYXw3Gk5SLIoqpA3WjWwANY-bcy5Vg2WOwIocWygbzmpsqWIMcg3x3lrXJVW8aKjmsqplmx-Qs9Vc6az3TmmxcGaObikoiN-sxF9W0Z6s7OKzmat2I9fhRHC6BugldtphL43fOF7lFcvz6NKVM_Hwr78-ug_BIinF8-OTGBejyfsrvInxZi5KL2b20_Uxkn8W_AG2N44K</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>Enzymatic Spectrophotometric Method for Aflatoxin B Detection Based on Acetylcholinesterase Inhibition</title><source>MEDLINE</source><source>ACS Publications</source><creator>Arduini, Fabiana ; Errico, Ilenia ; Amine, Aziz ; Micheli, Laura ; Palleschi, Giuseppe ; Moscone, Danila</creator><creatorcontrib>Arduini, Fabiana ; Errico, Ilenia ; Amine, Aziz ; Micheli, Laura ; Palleschi, Giuseppe ; Moscone, Danila</creatorcontrib><description>A new method for aflatoxin B (AFB) determination is proposed. The AFB determination is based on acetylcholinesterase (AChE) inhibition, and the AChE residual activity is determined using the colorimetric method (Ellman's method). Cholinesterases (ChEs) from various sources were tested using AFB1 as reference aflatoxin. AChE from electric eel has shown the highest sensitivity to AFB1, and it was chosen for the rest of the work. To select and optimize the analytical procedures, an investigation on the type of AChE inhibition by AFB1 was carried out. The AChE degree of inhibition by AFB1 was independent of the incubation time and the enzyme concentrations, showing the reversibility of the inhibition. This reversibility of the inhibition permits a rapid analysis of AFB1, requiring only 3 min. For the development of the AFB1 assay, the pH, the time of reaction, temperature, and substrate concentration were evaluated and optimized. The linear range of 10−60 ng mL-1 was determined. To evaluate the selectivity of this method, the cross-reactivity with other aflatoxins such as aflatoxin B2, aflatoxin G1, aflatoxin G2, and aflatoxin M1 was investigated. Finally, the suitability of the assay for AFB1 quantification in barley was evaluated. This study shows a new approach to detect aflatoxins based on enzyme inhibition and has advantages such as the ease of use, rapidity, and cost effectiveness. Thus, it could find a possible use as a screening method for this type of mycotoxins.</description><identifier>ISSN: 0003-2700</identifier><identifier>EISSN: 1520-6882</identifier><identifier>DOI: 10.1021/ac061819j</identifier><identifier>PMID: 17408242</identifier><identifier>CODEN: ANCHAM</identifier><language>eng</language><publisher>Washington, DC: American Chemical Society</publisher><subject>Acetylcholinesterase - chemistry ; Acetylcholinesterase - drug effects ; Aflatoxin B1 - analysis ; Aflatoxin B1 - pharmacology ; Analytical chemistry ; Animals ; Chemistry ; Electrophorus ; Enzyme Activation - drug effects ; Exact sciences and technology ; Methanol - chemistry ; Methanol - pharmacology ; Molecular Structure ; Sensitivity and Specificity ; Spectrometric and optical methods ; Spectrophotometry - methods ; Structure-Activity Relationship ; Temperature</subject><ispartof>Analytical chemistry (Washington), 2007-05, Vol.79 (9), p.3409-3415</ispartof><rights>Copyright © 2007 American Chemical Society</rights><rights>2007 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a447t-ba9bcb0af800388ceba53a0643ad05b869ad1e6603f08199f951e84b1f8c79cd3</citedby><cites>FETCH-LOGICAL-a447t-ba9bcb0af800388ceba53a0643ad05b869ad1e6603f08199f951e84b1f8c79cd3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/ac061819j$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/ac061819j$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>314,778,782,2754,27065,27913,27914,56727,56777</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&amp;idt=18737633$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17408242$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Arduini, Fabiana</creatorcontrib><creatorcontrib>Errico, Ilenia</creatorcontrib><creatorcontrib>Amine, Aziz</creatorcontrib><creatorcontrib>Micheli, Laura</creatorcontrib><creatorcontrib>Palleschi, Giuseppe</creatorcontrib><creatorcontrib>Moscone, Danila</creatorcontrib><title>Enzymatic Spectrophotometric Method for Aflatoxin B Detection Based on Acetylcholinesterase Inhibition</title><title>Analytical chemistry (Washington)</title><addtitle>Anal. Chem</addtitle><description>A new method for aflatoxin B (AFB) determination is proposed. The AFB determination is based on acetylcholinesterase (AChE) inhibition, and the AChE residual activity is determined using the colorimetric method (Ellman's method). Cholinesterases (ChEs) from various sources were tested using AFB1 as reference aflatoxin. AChE from electric eel has shown the highest sensitivity to AFB1, and it was chosen for the rest of the work. To select and optimize the analytical procedures, an investigation on the type of AChE inhibition by AFB1 was carried out. The AChE degree of inhibition by AFB1 was independent of the incubation time and the enzyme concentrations, showing the reversibility of the inhibition. This reversibility of the inhibition permits a rapid analysis of AFB1, requiring only 3 min. For the development of the AFB1 assay, the pH, the time of reaction, temperature, and substrate concentration were evaluated and optimized. The linear range of 10−60 ng mL-1 was determined. To evaluate the selectivity of this method, the cross-reactivity with other aflatoxins such as aflatoxin B2, aflatoxin G1, aflatoxin G2, and aflatoxin M1 was investigated. Finally, the suitability of the assay for AFB1 quantification in barley was evaluated. This study shows a new approach to detect aflatoxins based on enzyme inhibition and has advantages such as the ease of use, rapidity, and cost effectiveness. Thus, it could find a possible use as a screening method for this type of mycotoxins.</description><subject>Acetylcholinesterase - chemistry</subject><subject>Acetylcholinesterase - drug effects</subject><subject>Aflatoxin B1 - analysis</subject><subject>Aflatoxin B1 - pharmacology</subject><subject>Analytical chemistry</subject><subject>Animals</subject><subject>Chemistry</subject><subject>Electrophorus</subject><subject>Enzyme Activation - drug effects</subject><subject>Exact sciences and technology</subject><subject>Methanol - chemistry</subject><subject>Methanol - pharmacology</subject><subject>Molecular Structure</subject><subject>Sensitivity and Specificity</subject><subject>Spectrometric and optical methods</subject><subject>Spectrophotometry - methods</subject><subject>Structure-Activity Relationship</subject><subject>Temperature</subject><issn>0003-2700</issn><issn>1520-6882</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpt0EtLAzEQB_AgitbHwS8ge_HgYXWyj2z2WKtWoaLg8xZmswlN3W5KEsH66Y202IunDJkfw8yfkGMK5xQyeoESGOW0nm2RAS0zSBnn2TYZAECeZhXAHtn3fgZAKVC2S_ZoVQDPimxA9HX_vZxjMDJ5WigZnF1MbbBzFVz8uldhattEW5cMdYfBfpk-uUyuVIjU2FijV20Si6FUYdnJqe1Mr3xQLjaSu35qGvMLD8mOxs6ro_V7QF5urp9Ht-nkYXw3Gk5SLIoqpA3WjWwANY-bcy5Vg2WOwIocWygbzmpsqWIMcg3x3lrXJVW8aKjmsqplmx-Qs9Vc6az3TmmxcGaObikoiN-sxF9W0Z6s7OKzmat2I9fhRHC6BugldtphL43fOF7lFcvz6NKVM_Hwr78-ug_BIinF8-OTGBejyfsrvInxZi5KL2b20_Uxkn8W_AG2N44K</recordid><startdate>20070501</startdate><enddate>20070501</enddate><creator>Arduini, Fabiana</creator><creator>Errico, Ilenia</creator><creator>Amine, Aziz</creator><creator>Micheli, Laura</creator><creator>Palleschi, Giuseppe</creator><creator>Moscone, Danila</creator><general>American Chemical Society</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>20070501</creationdate><title>Enzymatic Spectrophotometric Method for Aflatoxin B Detection Based on Acetylcholinesterase Inhibition</title><author>Arduini, Fabiana ; Errico, Ilenia ; Amine, Aziz ; Micheli, Laura ; Palleschi, Giuseppe ; Moscone, Danila</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a447t-ba9bcb0af800388ceba53a0643ad05b869ad1e6603f08199f951e84b1f8c79cd3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Acetylcholinesterase - chemistry</topic><topic>Acetylcholinesterase - drug effects</topic><topic>Aflatoxin B1 - analysis</topic><topic>Aflatoxin B1 - pharmacology</topic><topic>Analytical chemistry</topic><topic>Animals</topic><topic>Chemistry</topic><topic>Electrophorus</topic><topic>Enzyme Activation - drug effects</topic><topic>Exact sciences and technology</topic><topic>Methanol - chemistry</topic><topic>Methanol - pharmacology</topic><topic>Molecular Structure</topic><topic>Sensitivity and Specificity</topic><topic>Spectrometric and optical methods</topic><topic>Spectrophotometry - methods</topic><topic>Structure-Activity Relationship</topic><topic>Temperature</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Arduini, Fabiana</creatorcontrib><creatorcontrib>Errico, Ilenia</creatorcontrib><creatorcontrib>Amine, Aziz</creatorcontrib><creatorcontrib>Micheli, Laura</creatorcontrib><creatorcontrib>Palleschi, Giuseppe</creatorcontrib><creatorcontrib>Moscone, Danila</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><jtitle>Analytical chemistry (Washington)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Arduini, Fabiana</au><au>Errico, Ilenia</au><au>Amine, Aziz</au><au>Micheli, Laura</au><au>Palleschi, Giuseppe</au><au>Moscone, Danila</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Enzymatic Spectrophotometric Method for Aflatoxin B Detection Based on Acetylcholinesterase Inhibition</atitle><jtitle>Analytical chemistry (Washington)</jtitle><addtitle>Anal. Chem</addtitle><date>2007-05-01</date><risdate>2007</risdate><volume>79</volume><issue>9</issue><spage>3409</spage><epage>3415</epage><pages>3409-3415</pages><issn>0003-2700</issn><eissn>1520-6882</eissn><coden>ANCHAM</coden><abstract>A new method for aflatoxin B (AFB) determination is proposed. The AFB determination is based on acetylcholinesterase (AChE) inhibition, and the AChE residual activity is determined using the colorimetric method (Ellman's method). Cholinesterases (ChEs) from various sources were tested using AFB1 as reference aflatoxin. AChE from electric eel has shown the highest sensitivity to AFB1, and it was chosen for the rest of the work. To select and optimize the analytical procedures, an investigation on the type of AChE inhibition by AFB1 was carried out. The AChE degree of inhibition by AFB1 was independent of the incubation time and the enzyme concentrations, showing the reversibility of the inhibition. This reversibility of the inhibition permits a rapid analysis of AFB1, requiring only 3 min. For the development of the AFB1 assay, the pH, the time of reaction, temperature, and substrate concentration were evaluated and optimized. The linear range of 10−60 ng mL-1 was determined. To evaluate the selectivity of this method, the cross-reactivity with other aflatoxins such as aflatoxin B2, aflatoxin G1, aflatoxin G2, and aflatoxin M1 was investigated. Finally, the suitability of the assay for AFB1 quantification in barley was evaluated. This study shows a new approach to detect aflatoxins based on enzyme inhibition and has advantages such as the ease of use, rapidity, and cost effectiveness. Thus, it could find a possible use as a screening method for this type of mycotoxins.</abstract><cop>Washington, DC</cop><pub>American Chemical Society</pub><pmid>17408242</pmid><doi>10.1021/ac061819j</doi><tpages>7</tpages></addata></record>
fulltext fulltext
identifier ISSN: 0003-2700
ispartof Analytical chemistry (Washington), 2007-05, Vol.79 (9), p.3409-3415
issn 0003-2700
1520-6882
language eng
recordid cdi_crossref_primary_10_1021_ac061819j
source MEDLINE; ACS Publications
subjects Acetylcholinesterase - chemistry
Acetylcholinesterase - drug effects
Aflatoxin B1 - analysis
Aflatoxin B1 - pharmacology
Analytical chemistry
Animals
Chemistry
Electrophorus
Enzyme Activation - drug effects
Exact sciences and technology
Methanol - chemistry
Methanol - pharmacology
Molecular Structure
Sensitivity and Specificity
Spectrometric and optical methods
Spectrophotometry - methods
Structure-Activity Relationship
Temperature
title Enzymatic Spectrophotometric Method for Aflatoxin B Detection Based on Acetylcholinesterase Inhibition
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-15T09%3A07%3A42IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-istex_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Enzymatic%20Spectrophotometric%20Method%20for%20Aflatoxin%20B%20Detection%20Based%20on%20Acetylcholinesterase%20Inhibition&rft.jtitle=Analytical%20chemistry%20(Washington)&rft.au=Arduini,%20Fabiana&rft.date=2007-05-01&rft.volume=79&rft.issue=9&rft.spage=3409&rft.epage=3415&rft.pages=3409-3415&rft.issn=0003-2700&rft.eissn=1520-6882&rft.coden=ANCHAM&rft_id=info:doi/10.1021/ac061819j&rft_dat=%3Cistex_cross%3Eark_67375_TPS_G4CLXV0W_G%3C/istex_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/17408242&rfr_iscdi=true