Effect of combined exposure to silica nanoparticles and cadmium chloride on female zebrafish ovaries

•Combined exposure of CdCl2+SiNPs for zebrafish lead to impaired varian structure.•SiNPs magnificated CdCl2 lead to development of malformed offspring.•Combined exposure of CdCl2+SiNPs altered lipid, estrogen and vitellogenin metabolism. Silica nanoparticles (SiNPs) and cadmium chloride (CdCl2) are two important environmental pollutants. In previous research, found that SiNPs in zebrafish larvae can amplify the cardiovascular damage caused by cadmium. Whether SiNPs in the ovaries can amplify the adverse effects of cadmium on the zebrafish ovaries is worth studying problem. In this study, sexually mature female zebrafish were used as model organisms and exposed to 1 μmol/L CdCl2 and/or 25 μg/mL SiNPs for 30 days. The results showed that the structure and function of ovaries in the sole and combined exposure groups changed significantly, resulting in reduced ovarian quality, decreased number of mature oocytes, and the development of malformed offspring. A deep-sequencing analysis showed that organisms’ lipid metabolism and transportation, estrogen metabolism, and response to the maturation, meiosis, and vitellogenin synthesis of oocytes were significantly affected by single exposure or combined exposure. These findings provide further insights into the harm of cooperation of CdCl2 and/or SiNPs to the aquatic ecosystems.