Molecular structure and bioactivities of 2, 4-Ditert butyl phenol extracted from Plumbago zeylanica, investigated using HPLC and NMR

The global potential of therapeutic plants in treating microbial illnesses is evident. This study focuses on the Plumbago zeylanica ( P.zeylanica )plant and aims to isolate and investigate a bioactive compound with antifungal, antioxidant, and cancer-fighting properties. The compound identified as 2,4-Ditert butyl phenol (2,4-DTBP) was successfully extracted from P. zeylanica using Ethyl acetate and confirmed through GC–MS analysis. The antioxidant effects of 2,4-DTBP are well-known as it effectively suppresses oxidation, preventing material degradation and disintegration. Its ability to neutralize free radicals and reduce reactive oxygen species production makes it valuable in stabilizing various compounds. Notably, it enhances the durability and endurance of plastics, rubber, and polymers. The isolation and purification of 2,4-DTBP were accomplished using HPLC, and its structure was characterized as C 14 H 22 O through 1H NMR analysis. The fungicidal activity of 2,4-DTBP against Botrytis cinerea was proven through agar plate assay, and its cytotoxic activity was evaluated using MCF-7 cells (a breast carcinoma cell line) with an identified IC 50 value of 5 μg/ml. This study provides insight into the medicinal properties of 2,4-DTPB and its effectiveness against cancer cells. Overall, further investigation and exploration of 2,4-DTBP's potential in cancer research would contribute to its potential application as a novel therapeutic agent in the future.