Enhancement of scopolamine production in Hyoscyamus muticus L. hairy root cultures by genetic engineering
In order to test the possibility of enhancing the production of pharmaceutically valuable scopolamine in transgenic cultures, the 35S-h6h transgene that codes for the enzyme hyoscyamine-6 beta-hydroxylase (EC 1.14.11.11) was introduced into Hyoscyamus muticus L. strain Cairo (Egyptian henbane). This...
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| Veröffentlicht in: | Planta 1999-06, Vol.208 (4), p.545-551 |
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| creator | Jouhikainen, K Lindgren, L Jokelainen, T Hiltunen, R Teeri, T.H Oksman-Caldentey, K.M |
| description | In order to test the possibility of enhancing the production of pharmaceutically valuable scopolamine in transgenic cultures, the 35S-h6h transgene that codes for the enzyme hyoscyamine-6 beta-hydroxylase (EC 1.14.11.11) was introduced into Hyoscyamus muticus L. strain Cairo (Egyptian henbane). This plant was chosen for its capability to produce very high amounts of tropane alkaloids (up to 6% of the dry weight in the leaves of mature plant). To our knowledge, this is the first time such a large population of transgenic cultures has been studied at the morphological, chemical and genetic levels. A great variation was observed in the tropane alkaloid production among the 43 positive transformants. The best clone (KB7) produced 17 mg/l scopolamine, which is over 100 times more than the control clones. However, conversion of hyoscyamine to scopolamine was still incomplete. The expression of h6h was found to be proportional to the scopolamine production, and was the main reason behind the variation in the scopolamine/hyoscyamine ratio in the hairy-root clones. These results indicate that H. muticus strain Cairo has a potential for even more enhanced scopolamine production with more efficient gene-expression systems. |
| doi_str_mv | 10.1007/s004250050592 |
| format | Article |
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This plant was chosen for its capability to produce very high amounts of tropane alkaloids (up to 6% of the dry weight in the leaves of mature plant). To our knowledge, this is the first time such a large population of transgenic cultures has been studied at the morphological, chemical and genetic levels. A great variation was observed in the tropane alkaloid production among the 43 positive transformants. The best clone (KB7) produced 17 mg/l scopolamine, which is over 100 times more than the control clones. However, conversion of hyoscyamine to scopolamine was still incomplete. The expression of h6h was found to be proportional to the scopolamine production, and was the main reason behind the variation in the scopolamine/hyoscyamine ratio in the hairy-root clones. These results indicate that H. muticus strain Cairo has a potential for even more enhanced scopolamine production with more efficient gene-expression systems.</description><identifier>ISSN: 0032-0935</identifier><identifier>EISSN: 1432-2048</identifier><identifier>DOI: 10.1007/s004250050592</identifier><identifier>CODEN: PLANAB</identifier><language>eng</language><publisher>Berlin: Springer-Verlag</publisher><subject>Agrobacterium ; Agronomy. Soil science and plant productions ; Alkaloids ; Amines ; Biological and medical sciences ; biosynthesis ; Biotechnology ; callus ; chemical constituents of plants ; Cultured cells ; Fundamental and applied biological sciences. Psychology ; gene expression ; genes ; genetic engineering ; Genetic engineering applications ; genetic variation ; Genetics and breeding of economic plants ; Hyoscyamus muticus ; oxygenases ; Plant breeding: fundamental aspects and methodology ; Plant cells ; plant morphology ; Plant roots ; Plants ; roots ; tissue culture ; transformation ; Transgenes ; Transgenic plants ; tropane alkaloids ; Tropanes</subject><ispartof>Planta, 1999-06, Vol.208 (4), p.545-551</ispartof><rights>Springer-Verlag Berlin Heidelberg 1999</rights><rights>1999 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c376t-fbc26ff45c93b8bec049145e2ed89def040884bd7703ef65d09a1885e62bad713</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/23385761$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/23385761$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>315,781,785,804,27928,27929,58021,58254</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1866350$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>Jouhikainen, K</creatorcontrib><creatorcontrib>Lindgren, L</creatorcontrib><creatorcontrib>Jokelainen, T</creatorcontrib><creatorcontrib>Hiltunen, R</creatorcontrib><creatorcontrib>Teeri, T.H</creatorcontrib><creatorcontrib>Oksman-Caldentey, K.M</creatorcontrib><title>Enhancement of scopolamine production in Hyoscyamus muticus L. hairy root cultures by genetic engineering</title><title>Planta</title><description>In order to test the possibility of enhancing the production of pharmaceutically valuable scopolamine in transgenic cultures, the 35S-h6h transgene that codes for the enzyme hyoscyamine-6 beta-hydroxylase (EC 1.14.11.11) was introduced into Hyoscyamus muticus L. strain Cairo (Egyptian henbane). This plant was chosen for its capability to produce very high amounts of tropane alkaloids (up to 6% of the dry weight in the leaves of mature plant). To our knowledge, this is the first time such a large population of transgenic cultures has been studied at the morphological, chemical and genetic levels. A great variation was observed in the tropane alkaloid production among the 43 positive transformants. The best clone (KB7) produced 17 mg/l scopolamine, which is over 100 times more than the control clones. However, conversion of hyoscyamine to scopolamine was still incomplete. The expression of h6h was found to be proportional to the scopolamine production, and was the main reason behind the variation in the scopolamine/hyoscyamine ratio in the hairy-root clones. These results indicate that H. muticus strain Cairo has a potential for even more enhanced scopolamine production with more efficient gene-expression systems.</description><subject>Agrobacterium</subject><subject>Agronomy. Soil science and plant productions</subject><subject>Alkaloids</subject><subject>Amines</subject><subject>Biological and medical sciences</subject><subject>biosynthesis</subject><subject>Biotechnology</subject><subject>callus</subject><subject>chemical constituents of plants</subject><subject>Cultured cells</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>gene expression</subject><subject>genes</subject><subject>genetic engineering</subject><subject>Genetic engineering applications</subject><subject>genetic variation</subject><subject>Genetics and breeding of economic plants</subject><subject>Hyoscyamus muticus</subject><subject>oxygenases</subject><subject>Plant breeding: fundamental aspects and methodology</subject><subject>Plant cells</subject><subject>plant morphology</subject><subject>Plant roots</subject><subject>Plants</subject><subject>roots</subject><subject>tissue culture</subject><subject>transformation</subject><subject>Transgenes</subject><subject>Transgenic plants</subject><subject>tropane alkaloids</subject><subject>Tropanes</subject><issn>0032-0935</issn><issn>1432-2048</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><recordid>eNpVkMFLwzAUh4MoOKdHj2IOXjtfk6ZNjzKmEwYedOeSpkmX0SYjaQ_9782oKJ7eg9_3fg8-hO5TWKUAxXMAyAgDYMBKcoEWaUZJQiDjl2gBEHcoKbtGNyEcAWJYFAtkNvYgrFS9sgN2GgfpTq4TvbEKn7xrRjkYZ7GxeDu5ICfRjwH342BknLsVPgjjJ-ydG7Acu2H0KuB6wq2yKjJY2TY2KW9se4uutOiCuvuZS7R_3Xytt8nu4-19_bJLJC3yIdG1JLnWGZMlrXmtJGRlmjFFVMPLRmnIgPOsbooCqNI5a6AUKedM5aQWTZHSJUrmXuldCF7p6uRNL_xUpVCdPVX_PEX-aeZPIkjRaR91mPB3xPOcMojYw4wdw-D8b0wo5azIz28f51wLV4nWx4r9J4GUAikJ5FH9N_wcfFA</recordid><startdate>19990601</startdate><enddate>19990601</enddate><creator>Jouhikainen, K</creator><creator>Lindgren, L</creator><creator>Jokelainen, T</creator><creator>Hiltunen, R</creator><creator>Teeri, T.H</creator><creator>Oksman-Caldentey, K.M</creator><general>Springer-Verlag</general><general>Springer</general><scope>FBQ</scope><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>19990601</creationdate><title>Enhancement of scopolamine production in Hyoscyamus muticus L. hairy root cultures by genetic engineering</title><author>Jouhikainen, K ; Lindgren, L ; Jokelainen, T ; Hiltunen, R ; Teeri, T.H ; Oksman-Caldentey, K.M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c376t-fbc26ff45c93b8bec049145e2ed89def040884bd7703ef65d09a1885e62bad713</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Agrobacterium</topic><topic>Agronomy. Soil science and plant productions</topic><topic>Alkaloids</topic><topic>Amines</topic><topic>Biological and medical sciences</topic><topic>biosynthesis</topic><topic>Biotechnology</topic><topic>callus</topic><topic>chemical constituents of plants</topic><topic>Cultured cells</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>gene expression</topic><topic>genes</topic><topic>genetic engineering</topic><topic>Genetic engineering applications</topic><topic>genetic variation</topic><topic>Genetics and breeding of economic plants</topic><topic>Hyoscyamus muticus</topic><topic>oxygenases</topic><topic>Plant breeding: fundamental aspects and methodology</topic><topic>Plant cells</topic><topic>plant morphology</topic><topic>Plant roots</topic><topic>Plants</topic><topic>roots</topic><topic>tissue culture</topic><topic>transformation</topic><topic>Transgenes</topic><topic>Transgenic plants</topic><topic>tropane alkaloids</topic><topic>Tropanes</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Jouhikainen, K</creatorcontrib><creatorcontrib>Lindgren, L</creatorcontrib><creatorcontrib>Jokelainen, T</creatorcontrib><creatorcontrib>Hiltunen, R</creatorcontrib><creatorcontrib>Teeri, T.H</creatorcontrib><creatorcontrib>Oksman-Caldentey, K.M</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>CrossRef</collection><jtitle>Planta</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Jouhikainen, K</au><au>Lindgren, L</au><au>Jokelainen, T</au><au>Hiltunen, R</au><au>Teeri, T.H</au><au>Oksman-Caldentey, K.M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Enhancement of scopolamine production in Hyoscyamus muticus L. hairy root cultures by genetic engineering</atitle><jtitle>Planta</jtitle><date>1999-06-01</date><risdate>1999</risdate><volume>208</volume><issue>4</issue><spage>545</spage><epage>551</epage><pages>545-551</pages><issn>0032-0935</issn><eissn>1432-2048</eissn><coden>PLANAB</coden><abstract>In order to test the possibility of enhancing the production of pharmaceutically valuable scopolamine in transgenic cultures, the 35S-h6h transgene that codes for the enzyme hyoscyamine-6 beta-hydroxylase (EC 1.14.11.11) was introduced into Hyoscyamus muticus L. strain Cairo (Egyptian henbane). This plant was chosen for its capability to produce very high amounts of tropane alkaloids (up to 6% of the dry weight in the leaves of mature plant). To our knowledge, this is the first time such a large population of transgenic cultures has been studied at the morphological, chemical and genetic levels. A great variation was observed in the tropane alkaloid production among the 43 positive transformants. The best clone (KB7) produced 17 mg/l scopolamine, which is over 100 times more than the control clones. However, conversion of hyoscyamine to scopolamine was still incomplete. The expression of h6h was found to be proportional to the scopolamine production, and was the main reason behind the variation in the scopolamine/hyoscyamine ratio in the hairy-root clones. These results indicate that H. muticus strain Cairo has a potential for even more enhanced scopolamine production with more efficient gene-expression systems.</abstract><cop>Berlin</cop><pub>Springer-Verlag</pub><doi>10.1007/s004250050592</doi><tpages>7</tpages></addata></record> |
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| subjects | Agrobacterium Agronomy. Soil science and plant productions Alkaloids Amines Biological and medical sciences biosynthesis Biotechnology callus chemical constituents of plants Cultured cells Fundamental and applied biological sciences. Psychology gene expression genes genetic engineering Genetic engineering applications genetic variation Genetics and breeding of economic plants Hyoscyamus muticus oxygenases Plant breeding: fundamental aspects and methodology Plant cells plant morphology Plant roots Plants roots tissue culture transformation Transgenes Transgenic plants tropane alkaloids Tropanes |
| title | Enhancement of scopolamine production in Hyoscyamus muticus L. hairy root cultures by genetic engineering |
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