Differential elicitation of defense responses by pectic fragments in bean seedlings

The cell walls of two near-isogenic lines of bean (Phaseolus vulgaris L.) seedlings, susceptible or resistant to the bean anthracnose pathogen Colletotrichum lindemuthianum, were digested with the pure endopolygalacturonase (endoPG; EC 3.2.1.15) isolated from the fungus. The solubilized pectic fragm...

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Veröffentlicht in:Planta 1998-09, Vol.206 (1), p.86-94
Hauptverfasser: Boudart, G, Lafitte, C, Barthe, J.P, Frasez, D, Esquerre-Tugaye, M.T
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creator Boudart, G
Lafitte, C
Barthe, J.P
Frasez, D
Esquerre-Tugaye, M.T
description The cell walls of two near-isogenic lines of bean (Phaseolus vulgaris L.) seedlings, susceptible or resistant to the bean anthracnose pathogen Colletotrichum lindemuthianum, were digested with the pure endopolygalacturonase (endoPG; EC 3.2.1.15) isolated from the fungus. The solubilized pectic fragments were separated according to their charge and size. Analysis of their uronic acid contents showed that their elution patterns were quite dissimilar, depending on whether they originated from the resistant or the susceptible host plant. Their sugar compositions revealed that neutral sugars were more abundant in the fragments released from the resistant plant than from the susceptible one, while the reverse was true for acidic residues. The fragments solubilized from the resistant plant induced an increase of pathogenesis-related (PR) proteins when challenged on resistant or susceptible bean seedlings, both at the transcript and enzyme-activity levels. On the other hand, pectic fragments released from susceptible bean cell walls exhibited either no significant activity or only a weak elicitor effect on the defence of susceptible or resistant bean seedlings. The differential elicitor effect observed between pectic fragments was inversely correlated to their acidity. Thus, endoPG-released pectic fragments from bean cell walls exhibited the same ability as the endoPG itself (C. Lafitte et al., 1993, Mol Plant-Microb Interact 6: 628-634) to elicit defence responses in a cultivar-specific manner.
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The solubilized pectic fragments were separated according to their charge and size. Analysis of their uronic acid contents showed that their elution patterns were quite dissimilar, depending on whether they originated from the resistant or the susceptible host plant. Their sugar compositions revealed that neutral sugars were more abundant in the fragments released from the resistant plant than from the susceptible one, while the reverse was true for acidic residues. The fragments solubilized from the resistant plant induced an increase of pathogenesis-related (PR) proteins when challenged on resistant or susceptible bean seedlings, both at the transcript and enzyme-activity levels. On the other hand, pectic fragments released from susceptible bean cell walls exhibited either no significant activity or only a weak elicitor effect on the defence of susceptible or resistant bean seedlings. The differential elicitor effect observed between pectic fragments was inversely correlated to their acidity. Thus, endoPG-released pectic fragments from bean cell walls exhibited the same ability as the endoPG itself (C. Lafitte et al., 1993, Mol Plant-Microb Interact 6: 628-634) to elicit defence responses in a cultivar-specific manner.</description><identifier>ISSN: 0032-0935</identifier><identifier>EISSN: 1432-2048</identifier><identifier>DOI: 10.1007/s004250050377</identifier><identifier>CODEN: PLANAB</identifier><language>eng</language><publisher>Berlin: Springer-Verlag</publisher><subject>acidity ; arabinose ; beta-glucanase ; Biological and medical sciences ; biological resistance ; cell wall components ; Cell walls ; chemical constituents of plants ; chitinase ; Chromatography ; Colletotrichum ; Colletotrichum lindemuthianum ; cultivars ; defense mechanisms ; disease resistance ; enzyme activity ; Enzymes ; Fundamental and applied biological sciences. Psychology ; Fungal plant pathogens ; Fungi ; galactose ; galacturonic acid ; Hydrolysis ; messenger RNA ; pathogenesis-related proteins ; Pathology, epidemiology, host-fungus relationships. Damages, economic importance ; pectins ; Phaseolus vulgaris ; Phytopathology. Animal pests. 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The solubilized pectic fragments were separated according to their charge and size. Analysis of their uronic acid contents showed that their elution patterns were quite dissimilar, depending on whether they originated from the resistant or the susceptible host plant. Their sugar compositions revealed that neutral sugars were more abundant in the fragments released from the resistant plant than from the susceptible one, while the reverse was true for acidic residues. The fragments solubilized from the resistant plant induced an increase of pathogenesis-related (PR) proteins when challenged on resistant or susceptible bean seedlings, both at the transcript and enzyme-activity levels. On the other hand, pectic fragments released from susceptible bean cell walls exhibited either no significant activity or only a weak elicitor effect on the defence of susceptible or resistant bean seedlings. 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Lafitte et al., 1993, Mol Plant-Microb Interact 6: 628-634) to elicit defence responses in a cultivar-specific manner.</description><subject>acidity</subject><subject>arabinose</subject><subject>beta-glucanase</subject><subject>Biological and medical sciences</subject><subject>biological resistance</subject><subject>cell wall components</subject><subject>Cell walls</subject><subject>chemical constituents of plants</subject><subject>chitinase</subject><subject>Chromatography</subject><subject>Colletotrichum</subject><subject>Colletotrichum lindemuthianum</subject><subject>cultivars</subject><subject>defense mechanisms</subject><subject>disease resistance</subject><subject>enzyme activity</subject><subject>Enzymes</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fungal plant pathogens</subject><subject>Fungi</subject><subject>galactose</subject><subject>galacturonic acid</subject><subject>Hydrolysis</subject><subject>messenger RNA</subject><subject>pathogenesis-related proteins</subject><subject>Pathology, epidemiology, host-fungus relationships. Damages, economic importance</subject><subject>pectins</subject><subject>Phaseolus vulgaris</subject><subject>Phytopathology. Animal pests. 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Psychology</topic><topic>Fungal plant pathogens</topic><topic>Fungi</topic><topic>galactose</topic><topic>galacturonic acid</topic><topic>Hydrolysis</topic><topic>messenger RNA</topic><topic>pathogenesis-related proteins</topic><topic>Pathology, epidemiology, host-fungus relationships. Damages, economic importance</topic><topic>pectins</topic><topic>Phaseolus vulgaris</topic><topic>Phytopathology. Animal pests. Plant and forest protection</topic><topic>Plant cells</topic><topic>Plants</topic><topic>polygalacturonase</topic><topic>rhamnose</topic><topic>Seedlings</topic><topic>Sugars</topic><topic>uronic acids</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Boudart, G</creatorcontrib><creatorcontrib>Lafitte, C</creatorcontrib><creatorcontrib>Barthe, J.P</creatorcontrib><creatorcontrib>Frasez, D</creatorcontrib><creatorcontrib>Esquerre-Tugaye, M.T</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>CrossRef</collection><jtitle>Planta</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Boudart, G</au><au>Lafitte, C</au><au>Barthe, J.P</au><au>Frasez, D</au><au>Esquerre-Tugaye, M.T</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Differential elicitation of defense responses by pectic fragments in bean seedlings</atitle><jtitle>Planta</jtitle><date>1998-09-01</date><risdate>1998</risdate><volume>206</volume><issue>1</issue><spage>86</spage><epage>94</epage><pages>86-94</pages><issn>0032-0935</issn><eissn>1432-2048</eissn><coden>PLANAB</coden><abstract>The cell walls of two near-isogenic lines of bean (Phaseolus vulgaris L.) seedlings, susceptible or resistant to the bean anthracnose pathogen Colletotrichum lindemuthianum, were digested with the pure endopolygalacturonase (endoPG; EC 3.2.1.15) isolated from the fungus. The solubilized pectic fragments were separated according to their charge and size. Analysis of their uronic acid contents showed that their elution patterns were quite dissimilar, depending on whether they originated from the resistant or the susceptible host plant. Their sugar compositions revealed that neutral sugars were more abundant in the fragments released from the resistant plant than from the susceptible one, while the reverse was true for acidic residues. The fragments solubilized from the resistant plant induced an increase of pathogenesis-related (PR) proteins when challenged on resistant or susceptible bean seedlings, both at the transcript and enzyme-activity levels. On the other hand, pectic fragments released from susceptible bean cell walls exhibited either no significant activity or only a weak elicitor effect on the defence of susceptible or resistant bean seedlings. The differential elicitor effect observed between pectic fragments was inversely correlated to their acidity. Thus, endoPG-released pectic fragments from bean cell walls exhibited the same ability as the endoPG itself (C. Lafitte et al., 1993, Mol Plant-Microb Interact 6: 628-634) to elicit defence responses in a cultivar-specific manner.</abstract><cop>Berlin</cop><pub>Springer-Verlag</pub><doi>10.1007/s004250050377</doi><tpages>9</tpages></addata></record>
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subjects acidity
arabinose
beta-glucanase
Biological and medical sciences
biological resistance
cell wall components
Cell walls
chemical constituents of plants
chitinase
Chromatography
Colletotrichum
Colletotrichum lindemuthianum
cultivars
defense mechanisms
disease resistance
enzyme activity
Enzymes
Fundamental and applied biological sciences. Psychology
Fungal plant pathogens
Fungi
galactose
galacturonic acid
Hydrolysis
messenger RNA
pathogenesis-related proteins
Pathology, epidemiology, host-fungus relationships. Damages, economic importance
pectins
Phaseolus vulgaris
Phytopathology. Animal pests. Plant and forest protection
Plant cells
Plants
polygalacturonase
rhamnose
Seedlings
Sugars
uronic acids
title Differential elicitation of defense responses by pectic fragments in bean seedlings
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