Nitrate ( 15 NO 3 ) limitation affects nitrogen partitioning between metabolic and storage sinks and nitrogen reserve accumulation in chicory (Cichorium intybus L.)

In chicory, we examined how $\mathrm{N}{\mathrm{O}}_{3}^{-}$ supply affected $\mathrm{N}{\mathrm{O}}_{3}^{-}$ uptake, N partitioning between shoot and root and N accumulation in the tuberized root throughout the vegetative period. Plants were grown at two $\mathrm{N}{\mathrm{O}}_{3}^{-}$ concentrati...

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Veröffentlicht in:Planta 1997-06, Vol.202 (3), p.303-312
Hauptverfasser: Améziane, Rafiqa, Richard-Molard, Céline, Deléens, Eliane, Morot-Gaudry, Jean-François, Limami, Anis M.
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container_start_page 303
container_title Planta
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creator Améziane, Rafiqa
Richard-Molard, Céline
Deléens, Eliane
Morot-Gaudry, Jean-François
Limami, Anis M.
description In chicory, we examined how $\mathrm{N}{\mathrm{O}}_{3}^{-}$ supply affected $\mathrm{N}{\mathrm{O}}_{3}^{-}$ uptake, N partitioning between shoot and root and N accumulation in the tuberized root throughout the vegetative period. Plants were grown at two $\mathrm{N}{\mathrm{O}}_{3}^{-}$ concentrations: 0.6 and 3 mM. We used 15N-labelling/chase experiments for the quantification of N fluxes between shoot and root and for determining whether N stored in the tuberized root originates from N remobilized from the shoot or from recently absorbed $\mathrm{N}{\mathrm{O}}_{3}^{-}$. The rate of ^{15}\mathrm{N}{\mathrm{O}}_{3}^{-}$ uptake was decreased by low $\mathrm{N}{\mathrm{O}}_{3}^{-}$ availability at all stages of growth. In young plants (10—55 days after sowing; DAS), in both $\mathrm{N}{\mathrm{O}}_{3}^{-}$ treatments the leaves were the strongest sink for 15N. In mature (tuberizing) plants, (55—115 DAS), the rate of ^{15}\mathrm{N}{\mathrm{O}}_{3}^{-}$ uptake increased as well as the amount of exogenous N allocated to the root. In N-limited plants, N allocation to the tuberized root relied essentially on recent N absorption, while in N-replete plants, N remobilized from the shoot contributed more to N-reverse accumulation in the root. In senescing plants (115—170 DAS) the rate of ^{15}\mathrm{N}{\mathrm{O}}_{3}^{-}$ uptake decreased mainly in N-replete plants whereas it remained almost unchanged in N-limited plants. In both $\mathrm{N}{\mathrm{O}}_{3}^{-}$ treatments the tuberized root was the strongest sink for recently absorbed N. Remobilization of previously absorbed N from shoot to tuberized root increased greatly in N-limited plants, whereas it increased slightly in N-replete plants. As a consequence, accumulation of the N-storage compounds vegetative storage protein (VSP) and arginine was delayed until later in the vegetative period in N-limited plants. Our results show that although the dynamics of N storage was affected by $\mathrm{N}{\mathrm{O}}_{3}^{-}$ supply, the final content of total N, VSP and arginine in roots was almost the same in N-limited and N-replete plants. This indicates that chicory is able to build up a store of available N-reserves, even when plants are grown on low N. We also suggest that in tuberized roots there is a maximal capacity for N accumulation, which was reached earlier (soon after 100 DAS) in N-replete plants. This hypothesis is supported by the fact that in N-replete plants despite $\mathrm{N}{\mathrm{O}}_{3}^{-}$ av
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Plants were grown at two $\mathrm{N}{\mathrm{O}}_{3}^{-}$ concentrations: 0.6 and 3 mM. We used 15N-labelling/chase experiments for the quantification of N fluxes between shoot and root and for determining whether N stored in the tuberized root originates from N remobilized from the shoot or from recently absorbed $\mathrm{N}{\mathrm{O}}_{3}^{-}$. The rate of ^{15}\mathrm{N}{\mathrm{O}}_{3}^{-}$ uptake was decreased by low $\mathrm{N}{\mathrm{O}}_{3}^{-}$ availability at all stages of growth. In young plants (10—55 days after sowing; DAS), in both $\mathrm{N}{\mathrm{O}}_{3}^{-}$ treatments the leaves were the strongest sink for 15N. In mature (tuberizing) plants, (55—115 DAS), the rate of ^{15}\mathrm{N}{\mathrm{O}}_{3}^{-}$ uptake increased as well as the amount of exogenous N allocated to the root. In N-limited plants, N allocation to the tuberized root relied essentially on recent N absorption, while in N-replete plants, N remobilized from the shoot contributed more to N-reverse accumulation in the root. In senescing plants (115—170 DAS) the rate of ^{15}\mathrm{N}{\mathrm{O}}_{3}^{-}$ uptake decreased mainly in N-replete plants whereas it remained almost unchanged in N-limited plants. In both $\mathrm{N}{\mathrm{O}}_{3}^{-}$ treatments the tuberized root was the strongest sink for recently absorbed N. Remobilization of previously absorbed N from shoot to tuberized root increased greatly in N-limited plants, whereas it increased slightly in N-replete plants. As a consequence, accumulation of the N-storage compounds vegetative storage protein (VSP) and arginine was delayed until later in the vegetative period in N-limited plants. Our results show that although the dynamics of N storage was affected by $\mathrm{N}{\mathrm{O}}_{3}^{-}$ supply, the final content of total N, VSP and arginine in roots was almost the same in N-limited and N-replete plants. This indicates that chicory is able to build up a store of available N-reserves, even when plants are grown on low N. We also suggest that in tuberized roots there is a maximal capacity for N accumulation, which was reached earlier (soon after 100 DAS) in N-replete plants. This hypothesis is supported by the fact that in N-replete plants despite $\mathrm{N}{\mathrm{O}}_{3}^{-}$ availability, N accumulation ceased and significant amounts of N were lost due to N efflux.</description><identifier>ISSN: 0032-0935</identifier><identifier>EISSN: 1432-2048</identifier><identifier>DOI: 10.1007/s004250050132</identifier><identifier>CODEN: PLANAB</identifier><language>eng</language><publisher>Berlin: Springer-Verlag</publisher><subject>Agronomy. 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Plants were grown at two $\mathrm{N}{\mathrm{O}}_{3}^{-}$ concentrations: 0.6 and 3 mM. We used 15N-labelling/chase experiments for the quantification of N fluxes between shoot and root and for determining whether N stored in the tuberized root originates from N remobilized from the shoot or from recently absorbed $\mathrm{N}{\mathrm{O}}_{3}^{-}$. The rate of ^{15}\mathrm{N}{\mathrm{O}}_{3}^{-}$ uptake was decreased by low $\mathrm{N}{\mathrm{O}}_{3}^{-}$ availability at all stages of growth. In young plants (10—55 days after sowing; DAS), in both $\mathrm{N}{\mathrm{O}}_{3}^{-}$ treatments the leaves were the strongest sink for 15N. In mature (tuberizing) plants, (55—115 DAS), the rate of ^{15}\mathrm{N}{\mathrm{O}}_{3}^{-}$ uptake increased as well as the amount of exogenous N allocated to the root. In N-limited plants, N allocation to the tuberized root relied essentially on recent N absorption, while in N-replete plants, N remobilized from the shoot contributed more to N-reverse accumulation in the root. In senescing plants (115—170 DAS) the rate of ^{15}\mathrm{N}{\mathrm{O}}_{3}^{-}$ uptake decreased mainly in N-replete plants whereas it remained almost unchanged in N-limited plants. In both $\mathrm{N}{\mathrm{O}}_{3}^{-}$ treatments the tuberized root was the strongest sink for recently absorbed N. Remobilization of previously absorbed N from shoot to tuberized root increased greatly in N-limited plants, whereas it increased slightly in N-replete plants. As a consequence, accumulation of the N-storage compounds vegetative storage protein (VSP) and arginine was delayed until later in the vegetative period in N-limited plants. Our results show that although the dynamics of N storage was affected by $\mathrm{N}{\mathrm{O}}_{3}^{-}$ supply, the final content of total N, VSP and arginine in roots was almost the same in N-limited and N-replete plants. This indicates that chicory is able to build up a store of available N-reserves, even when plants are grown on low N. We also suggest that in tuberized roots there is a maximal capacity for N accumulation, which was reached earlier (soon after 100 DAS) in N-replete plants. This hypothesis is supported by the fact that in N-replete plants despite $\mathrm{N}{\mathrm{O}}_{3}^{-}$ availability, N accumulation ceased and significant amounts of N were lost due to N efflux.</description><subject>Agronomy. Soil science and plant productions</subject><subject>Amino acids</subject><subject>Biological and medical sciences</subject><subject>Economic plant physiology</subject><subject>Fructans</subject><subject>Fundamental and applied biological sciences. 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Metabolism</topic><topic>Plant growth</topic><topic>Plant physiology and development</topic><topic>Plant roots</topic><topic>Plants</topic><topic>Storage proteins</topic><topic>Vegetative growth</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Améziane, Rafiqa</creatorcontrib><creatorcontrib>Richard-Molard, Céline</creatorcontrib><creatorcontrib>Deléens, Eliane</creatorcontrib><creatorcontrib>Morot-Gaudry, Jean-François</creatorcontrib><creatorcontrib>Limami, Anis M.</creatorcontrib><collection>Pascal-Francis</collection><collection>CrossRef</collection><jtitle>Planta</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Améziane, Rafiqa</au><au>Richard-Molard, Céline</au><au>Deléens, Eliane</au><au>Morot-Gaudry, Jean-François</au><au>Limami, Anis M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Nitrate ( 15 NO 3 ) limitation affects nitrogen partitioning between metabolic and storage sinks and nitrogen reserve accumulation in chicory (Cichorium intybus L.)</atitle><jtitle>Planta</jtitle><date>1997-06-23</date><risdate>1997</risdate><volume>202</volume><issue>3</issue><spage>303</spage><epage>312</epage><pages>303-312</pages><issn>0032-0935</issn><eissn>1432-2048</eissn><coden>PLANAB</coden><abstract>In chicory, we examined how $\mathrm{N}{\mathrm{O}}_{3}^{-}$ supply affected $\mathrm{N}{\mathrm{O}}_{3}^{-}$ uptake, N partitioning between shoot and root and N accumulation in the tuberized root throughout the vegetative period. Plants were grown at two $\mathrm{N}{\mathrm{O}}_{3}^{-}$ concentrations: 0.6 and 3 mM. We used 15N-labelling/chase experiments for the quantification of N fluxes between shoot and root and for determining whether N stored in the tuberized root originates from N remobilized from the shoot or from recently absorbed $\mathrm{N}{\mathrm{O}}_{3}^{-}$. The rate of ^{15}\mathrm{N}{\mathrm{O}}_{3}^{-}$ uptake was decreased by low $\mathrm{N}{\mathrm{O}}_{3}^{-}$ availability at all stages of growth. In young plants (10—55 days after sowing; DAS), in both $\mathrm{N}{\mathrm{O}}_{3}^{-}$ treatments the leaves were the strongest sink for 15N. In mature (tuberizing) plants, (55—115 DAS), the rate of ^{15}\mathrm{N}{\mathrm{O}}_{3}^{-}$ uptake increased as well as the amount of exogenous N allocated to the root. In N-limited plants, N allocation to the tuberized root relied essentially on recent N absorption, while in N-replete plants, N remobilized from the shoot contributed more to N-reverse accumulation in the root. In senescing plants (115—170 DAS) the rate of ^{15}\mathrm{N}{\mathrm{O}}_{3}^{-}$ uptake decreased mainly in N-replete plants whereas it remained almost unchanged in N-limited plants. In both $\mathrm{N}{\mathrm{O}}_{3}^{-}$ treatments the tuberized root was the strongest sink for recently absorbed N. Remobilization of previously absorbed N from shoot to tuberized root increased greatly in N-limited plants, whereas it increased slightly in N-replete plants. As a consequence, accumulation of the N-storage compounds vegetative storage protein (VSP) and arginine was delayed until later in the vegetative period in N-limited plants. Our results show that although the dynamics of N storage was affected by $\mathrm{N}{\mathrm{O}}_{3}^{-}$ supply, the final content of total N, VSP and arginine in roots was almost the same in N-limited and N-replete plants. This indicates that chicory is able to build up a store of available N-reserves, even when plants are grown on low N. We also suggest that in tuberized roots there is a maximal capacity for N accumulation, which was reached earlier (soon after 100 DAS) in N-replete plants. This hypothesis is supported by the fact that in N-replete plants despite $\mathrm{N}{\mathrm{O}}_{3}^{-}$ availability, N accumulation ceased and significant amounts of N were lost due to N efflux.</abstract><cop>Berlin</cop><pub>Springer-Verlag</pub><doi>10.1007/s004250050132</doi><tpages>10</tpages></addata></record>
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subjects Agronomy. Soil science and plant productions
Amino acids
Biological and medical sciences
Economic plant physiology
Fructans
Fundamental and applied biological sciences. Psychology
Leaves
Metabolism
Nitrates
Nitrogen
Nitrogen metabolism
Nitrogen metabolism and other ones (excepting carbon metabolism)
Nutrition. Photosynthesis. Respiration. Metabolism
Plant growth
Plant physiology and development
Plant roots
Plants
Storage proteins
Vegetative growth
title Nitrate ( 15 NO 3 ) limitation affects nitrogen partitioning between metabolic and storage sinks and nitrogen reserve accumulation in chicory (Cichorium intybus L.)
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