Interaction of biochemical quality and particle size of crop residues and its effect on the microbial biomass and nitrogen dynamics following incorporation into soil

Mineralization of N from organic materials added to soil depends on the quality of the substrate as a carbon, energy and nutrient source for the saprophytic microflora. Quality reflects a combination of biochemical and physical attributes. We investigated how biochemical composition interacts with particle size to affect the soil microflora and N dynamics following incorporation of crop residues into soil. Four fresh shoot and root crop residues were cut into coarse and fine particle sizes, and incorporated into sandy-loam soil which was incubated under controlled environment conditions for 6 months. In the case of the highest biochemical quality material, potato shoot (C/N ratio of 10:1), particle size had no effect on microbial respiration or net N mineralization. For lower biochemical quality Brussels sprout shoot (C/N ratio of 15:1), reducing particle size caused microbial respiration to peak earlier and increased net mineralization of N during the early stages of decomposition, but reduced net N mineralization at later stages. However, for the lowest biochemical quality residues, rye grass roots (C/N ratio of 38:1) and straw (C/N ratio of 91:1) reducing particle size caused microbial respiration to peak later and increased net immobilization of N. For Brussels sprout shoot, reducing particle size decreased the C content and the C/N ratio of residue-derived light fraction organic matter (LFOM) 2 months following incorporation. However C and N content of LFOM derived from the other materials was not affected by particle size. For materials of all qualities, particle size had little effect on biomass N. We conclude that the impact of particle size on soil microbial activities, and the protection of senescent microbial tissues from microbial attack, is dependent on the biochemical quality of the substrate.