Lead resistance in two bacterial isolates from heavy metal-contaminated soils
Microorganisms have developed mechanisms of coping with a variety of toxic metals; however, few studies have explored microbial resistance to lead. In this study, the overall mechanisms of a lead-resistant Pseudomonas marginalis and a lead-resistant Bacillus megaterium isolated from two different me...
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| Veröffentlicht in: | Microbial ecology 1999-04, Vol.37 (3), p.218-224 |
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| container_title | Microbial ecology |
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| creator | Roane, T.M |
| description | Microorganisms have developed mechanisms of coping with a variety of toxic metals; however, few studies have explored microbial resistance to lead. In this study, the overall mechanisms of a lead-resistant Pseudomonas marginalis and a lead-resistant Bacillus megaterium isolated from two different metal-contaminated soils were investigated. The P. marginalis had a higher lead resistance level at 2.5 mM total lead as compared to 0.6 mM for B. megaterium. Resistance to soluble lead was much lower, 0.3 and 0.1 mM, respectively. The degree of lead resistance and the mechanism of lead resistance for these two isolates corresponded with their environmental lead exposure. When viewed with transmission electron microscopy, P. marginalis, isolated from a soil contaminated with high total but undetectable soluble lead, showed extracellular lead exclusion. B. megaterium, from a soil with both high total and soluble lead levels, was less resistant with an intracellular cytoplasmic accumulation of lead as observed with TEM. Polarization microscopy indicated that while P. marginalis produced a high amount of an extracellular polymer implicated in the organism's mechanism of lead resistance, B. megaterium produced no discernable extracellular polymeric substances. The study of these two organisms demonstrated differences in how soil microorganisms respond to environmental lead exposure, including the novel mechanism of intracellular sequestration of lead. |
| doi_str_mv | 10.1007/s002489900145 |
| format | Article |
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In this study, the overall mechanisms of a lead-resistant Pseudomonas marginalis and a lead-resistant Bacillus megaterium isolated from two different metal-contaminated soils were investigated. The P. marginalis had a higher lead resistance level at 2.5 mM total lead as compared to 0.6 mM for B. megaterium. Resistance to soluble lead was much lower, 0.3 and 0.1 mM, respectively. The degree of lead resistance and the mechanism of lead resistance for these two isolates corresponded with their environmental lead exposure. When viewed with transmission electron microscopy, P. marginalis, isolated from a soil contaminated with high total but undetectable soluble lead, showed extracellular lead exclusion. B. megaterium, from a soil with both high total and soluble lead levels, was less resistant with an intracellular cytoplasmic accumulation of lead as observed with TEM. Polarization microscopy indicated that while P. marginalis produced a high amount of an extracellular polymer implicated in the organism's mechanism of lead resistance, B. megaterium produced no discernable extracellular polymeric substances. The study of these two organisms demonstrated differences in how soil microorganisms respond to environmental lead exposure, including the novel mechanism of intracellular sequestration of lead.</description><identifier>ISSN: 0095-3628</identifier><identifier>EISSN: 1432-184X</identifier><identifier>DOI: 10.1007/s002489900145</identifier><identifier>PMID: 10227879</identifier><identifier>CODEN: MCBEBU</identifier><language>eng</language><publisher>New York, NY: Springer-Verlag New York Inc</publisher><subject>Acid soils ; Action of physical and chemical agents on bacteria ; Agronomy. 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In this study, the overall mechanisms of a lead-resistant Pseudomonas marginalis and a lead-resistant Bacillus megaterium isolated from two different metal-contaminated soils were investigated. The P. marginalis had a higher lead resistance level at 2.5 mM total lead as compared to 0.6 mM for B. megaterium. Resistance to soluble lead was much lower, 0.3 and 0.1 mM, respectively. The degree of lead resistance and the mechanism of lead resistance for these two isolates corresponded with their environmental lead exposure. When viewed with transmission electron microscopy, P. marginalis, isolated from a soil contaminated with high total but undetectable soluble lead, showed extracellular lead exclusion. B. megaterium, from a soil with both high total and soluble lead levels, was less resistant with an intracellular cytoplasmic accumulation of lead as observed with TEM. Polarization microscopy indicated that while P. marginalis produced a high amount of an extracellular polymer implicated in the organism's mechanism of lead resistance, B. megaterium produced no discernable extracellular polymeric substances. The study of these two organisms demonstrated differences in how soil microorganisms respond to environmental lead exposure, including the novel mechanism of intracellular sequestration of lead.</description><subject>Acid soils</subject><subject>Action of physical and chemical agents on bacteria</subject><subject>Agronomy. Soil science and plant productions</subject><subject>Bacteriology</subject><subject>Bioavailability</subject><subject>Biochemistry and biology</subject><subject>Biological and medical sciences</subject><subject>Chemical, physicochemical, biochemical and biological properties</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>heavy metals</subject><subject>Lead</subject><subject>metal tolerance</subject><subject>Microbiology</subject><subject>Physics, chemistry, biochemistry and biology of agricultural and forest soils</subject><subject>polluted soils</subject><subject>Resistance mechanisms</subject><subject>Sedimentary soils</subject><subject>Soil bacteria</subject><subject>Soil mechanics</subject><subject>Soil microorganisms</subject><subject>Soil pollution</subject><subject>Soil science</subject><subject>Soil toxicity</subject><issn>0095-3628</issn><issn>1432-184X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><recordid>eNpV0EtLw0AUBeBBFFurS3eis3AbvfPIPJZSfEHFhRbchdvJRFOSpsxEpf_ekRQfq7s4HwfuIeSYwQUD0JcRgEtjLQCT-Q4ZMyl4xox82SVjAJtnQnEzIgcxLhPRiot9MmLAuTbajsnDzGNJg4917HHlPK1XtP_s6AJd70ONDa1j12DvI61C19I3jx8b2voem8x1qx7bepXSksaubuIh2auwif5oeydkfnP9PL3LZo-399OrWeaEyvuMy0oo73gpjQQweXrAK6-B64VjzKC2SnBVoXWVcAy9ZdKpXEnPvYBcl2JCsqHXhS7G4KtiHeoWw6ZgUHzPUvybJfnTwa_fF60v_-hhhwTOtwCjw6YKaYs6_jqTg9EqsZOBLWPfhZ9Y8pwpyVN8NsQVdgW-htQwf-LABHDLrGUgvgAL1Xvq</recordid><startdate>19990401</startdate><enddate>19990401</enddate><creator>Roane, T.M</creator><general>Springer-Verlag New York Inc</general><general>Springer</general><scope>FBQ</scope><scope>IQODW</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>19990401</creationdate><title>Lead resistance in two bacterial isolates from heavy metal-contaminated soils</title><author>Roane, T.M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c365t-24f36ec2d4840085248e6e7027bc118a796326fa9cf3c1ae914c6564e2e3057d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Acid soils</topic><topic>Action of physical and chemical agents on bacteria</topic><topic>Agronomy. Soil science and plant productions</topic><topic>Bacteriology</topic><topic>Bioavailability</topic><topic>Biochemistry and biology</topic><topic>Biological and medical sciences</topic><topic>Chemical, physicochemical, biochemical and biological properties</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>heavy metals</topic><topic>Lead</topic><topic>metal tolerance</topic><topic>Microbiology</topic><topic>Physics, chemistry, biochemistry and biology of agricultural and forest soils</topic><topic>polluted soils</topic><topic>Resistance mechanisms</topic><topic>Sedimentary soils</topic><topic>Soil bacteria</topic><topic>Soil mechanics</topic><topic>Soil microorganisms</topic><topic>Soil pollution</topic><topic>Soil science</topic><topic>Soil toxicity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Roane, T.M</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>PubMed</collection><collection>CrossRef</collection><jtitle>Microbial ecology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Roane, T.M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Lead resistance in two bacterial isolates from heavy metal-contaminated soils</atitle><jtitle>Microbial ecology</jtitle><addtitle>Microb Ecol</addtitle><date>1999-04-01</date><risdate>1999</risdate><volume>37</volume><issue>3</issue><spage>218</spage><epage>224</epage><pages>218-224</pages><issn>0095-3628</issn><eissn>1432-184X</eissn><coden>MCBEBU</coden><abstract>Microorganisms have developed mechanisms of coping with a variety of toxic metals; however, few studies have explored microbial resistance to lead. In this study, the overall mechanisms of a lead-resistant Pseudomonas marginalis and a lead-resistant Bacillus megaterium isolated from two different metal-contaminated soils were investigated. The P. marginalis had a higher lead resistance level at 2.5 mM total lead as compared to 0.6 mM for B. megaterium. Resistance to soluble lead was much lower, 0.3 and 0.1 mM, respectively. The degree of lead resistance and the mechanism of lead resistance for these two isolates corresponded with their environmental lead exposure. When viewed with transmission electron microscopy, P. marginalis, isolated from a soil contaminated with high total but undetectable soluble lead, showed extracellular lead exclusion. B. megaterium, from a soil with both high total and soluble lead levels, was less resistant with an intracellular cytoplasmic accumulation of lead as observed with TEM. Polarization microscopy indicated that while P. marginalis produced a high amount of an extracellular polymer implicated in the organism's mechanism of lead resistance, B. megaterium produced no discernable extracellular polymeric substances. The study of these two organisms demonstrated differences in how soil microorganisms respond to environmental lead exposure, including the novel mechanism of intracellular sequestration of lead.</abstract><cop>New York, NY</cop><pub>Springer-Verlag New York Inc</pub><pmid>10227879</pmid><doi>10.1007/s002489900145</doi><tpages>7</tpages></addata></record> |
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| ispartof | Microbial ecology, 1999-04, Vol.37 (3), p.218-224 |
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| subjects | Acid soils Action of physical and chemical agents on bacteria Agronomy. Soil science and plant productions Bacteriology Bioavailability Biochemistry and biology Biological and medical sciences Chemical, physicochemical, biochemical and biological properties Fundamental and applied biological sciences. Psychology heavy metals Lead metal tolerance Microbiology Physics, chemistry, biochemistry and biology of agricultural and forest soils polluted soils Resistance mechanisms Sedimentary soils Soil bacteria Soil mechanics Soil microorganisms Soil pollution Soil science Soil toxicity |
| title | Lead resistance in two bacterial isolates from heavy metal-contaminated soils |
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