Simvastatin protects diabetic rats against kidney injury through the suppression of renal matrix metalloproteinase-9 expression
Objective : To observe the effects of simvastatin on urinary excretion of matrix metalloproteinase-9 (MMP-9), renal expression of MMP-9, and investigate its possible renoprotective mechanisms in streptozotocin (STZ)-induced diabetic rats. Method : Twenty-four Wistar rats were divided into 3 groups:...
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| Veröffentlicht in: | Journal of endocrinological investigation 2010-05, Vol.33 (5), p.292-296 |
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| container_title | Journal of endocrinological investigation |
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| creator | Yao, X. M. Ye, S. D. Zai, Z. Chen, Y. Li, X. C. Yang, G. W. Wang, Y. X. Chen, K. |
| description | Objective
: To observe the effects of simvastatin on urinary excretion of matrix metalloproteinase-9 (MMP-9), renal expression of MMP-9, and investigate its possible renoprotective mechanisms in streptozotocin (STZ)-induced diabetic rats.
Method
: Twenty-four Wistar rats were divided into 3 groups: control healthy rats (group C, no.=8), untreated diabetic rats (group D, no.=8), and diabetic rats treated with simvastatin (20 mg/kg/d) (group S, no.=8). Peripheral blood glucose was tested weekly, glycosylated hemoglobin A
1c
(HbA
1c
), total cholesterol (TC), LDL cholesterol (LDL-C) levels, and urinary albumin (ALB) excretion rate as well as the urinary excretion rates of retinol-binding protein (RBP) and MMP-9 were tested at 8
th
week. The renal tissues of diabetic rats were obtained for evaluating kidney/body weight ratio, observing renal pathological changes by electron microscope and examining the expression of renal MMP-9 mRNA by RT-PCR.
Results
: There was no statistical difference on the change of peripheral blood TC and LDL-C between group C and group D. Peripheral blood glucose, HbA
1c
levels kidney/body weight ratio urinary excretion rates of ALB, RBP, and MMP-9 concurrently with the expression of renal MMP-9 mRNA were significantly higher in groups D and S compared with group C (
p |
| doi_str_mv | 10.1007/BF03346588 |
| format | Article |
| fullrecord | <record><control><sourceid>crossref_sprin</sourceid><recordid>TN_cdi_crossref_primary_10_1007_BF03346588</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>10_1007_BF03346588</sourcerecordid><originalsourceid>FETCH-LOGICAL-c197t-850c3ddb78d6183d3da3f9c553e9b91a7d48488753cc44e3b90e217d7505232f3</originalsourceid><addsrcrecordid>eNpt0EFLwzAYBuAgCs7pxV-Qs1JN-jVLctThVBh4UM8lTdIts01Lksp28q9bneLF08sHDy98L0LnlFxRQvj17YIAFDMmxAGaUJ6TTICYHaIJAUmzgkh-jE5i3BACHASfoI9n176rmFRyHvehS1aniI1TlU1O46DGS62U8zHhN2e83WHnN0PY4bQO3bBaj2lxHPo-2Bhd53FX42C9anCrUnBb3Nqkmqb77nZeRZtJbLe__BQd1aqJ9uwnp-h1cfcyf8iWT_eP85tlpqnkKROMaDCm4sLMqAADRkEtNWNgZSWp4qYQhRCcgdZFYaGSxOaUG84IyyGvYYou9r06dDEGW5d9cK0Ku5KS8mu68m-6EV_ucRyRX9lQbrohjD_F__QnvuVyGA</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>Simvastatin protects diabetic rats against kidney injury through the suppression of renal matrix metalloproteinase-9 expression</title><source>SpringerLink Journals - AutoHoldings</source><creator>Yao, X. M. ; Ye, S. D. ; Zai, Z. ; Chen, Y. ; Li, X. C. ; Yang, G. W. ; Wang, Y. X. ; Chen, K.</creator><creatorcontrib>Yao, X. M. ; Ye, S. D. ; Zai, Z. ; Chen, Y. ; Li, X. C. ; Yang, G. W. ; Wang, Y. X. ; Chen, K.</creatorcontrib><description>Objective
: To observe the effects of simvastatin on urinary excretion of matrix metalloproteinase-9 (MMP-9), renal expression of MMP-9, and investigate its possible renoprotective mechanisms in streptozotocin (STZ)-induced diabetic rats.
Method
: Twenty-four Wistar rats were divided into 3 groups: control healthy rats (group C, no.=8), untreated diabetic rats (group D, no.=8), and diabetic rats treated with simvastatin (20 mg/kg/d) (group S, no.=8). Peripheral blood glucose was tested weekly, glycosylated hemoglobin A
1c
(HbA
1c
), total cholesterol (TC), LDL cholesterol (LDL-C) levels, and urinary albumin (ALB) excretion rate as well as the urinary excretion rates of retinol-binding protein (RBP) and MMP-9 were tested at 8
th
week. The renal tissues of diabetic rats were obtained for evaluating kidney/body weight ratio, observing renal pathological changes by electron microscope and examining the expression of renal MMP-9 mRNA by RT-PCR.
Results
: There was no statistical difference on the change of peripheral blood TC and LDL-C between group C and group D. Peripheral blood glucose, HbA
1c
levels kidney/body weight ratio urinary excretion rates of ALB, RBP, and MMP-9 concurrently with the expression of renal MMP-9 mRNA were significantly higher in groups D and S compared with group C (
p
<0.01). Treatment with simvastatin significantly lowered peripheral blood TC, LDL-C, kidney/body weight ratio, urinary excretion rates of ALB, RBP, and MMP-9 as well as the expression of renal MMP-9 mRNA (
p
<0.01); however, there was no evident effect on the change of blood glucose and HbA
1c
levels between group D and group S. In addition, urinary excretion rate of MMP-9 showed positive correlations with the urinary ALB excretion and urinary RBP excretion. Pathological lesions of the glomeruli and epithelial cells foot processes (FP) was lightened by simvastatin.
Conclusion
: Simvastatin may has a potential therapeutic target in diabetic nephropathy, which may be partly attributed to down-regulating over-expression of MMP-9 in renal tissue.</description><identifier>ISSN: 0391-4097</identifier><identifier>EISSN: 1720-8386</identifier><identifier>DOI: 10.1007/BF03346588</identifier><language>eng</language><publisher>Cham: Springer International Publishing</publisher><subject>Endocrinology ; Medicine ; Medicine & Public Health ; Metabolic Diseases ; Original Articles</subject><ispartof>Journal of endocrinological investigation, 2010-05, Vol.33 (5), p.292-296</ispartof><rights>Italian Society of Endocrinology (SIE) 2010</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c197t-850c3ddb78d6183d3da3f9c553e9b91a7d48488753cc44e3b90e217d7505232f3</citedby><cites>FETCH-LOGICAL-c197t-850c3ddb78d6183d3da3f9c553e9b91a7d48488753cc44e3b90e217d7505232f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/BF03346588$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/BF03346588$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,777,781,27905,27906,41469,42538,51300</link.rule.ids></links><search><creatorcontrib>Yao, X. M.</creatorcontrib><creatorcontrib>Ye, S. D.</creatorcontrib><creatorcontrib>Zai, Z.</creatorcontrib><creatorcontrib>Chen, Y.</creatorcontrib><creatorcontrib>Li, X. C.</creatorcontrib><creatorcontrib>Yang, G. W.</creatorcontrib><creatorcontrib>Wang, Y. X.</creatorcontrib><creatorcontrib>Chen, K.</creatorcontrib><title>Simvastatin protects diabetic rats against kidney injury through the suppression of renal matrix metalloproteinase-9 expression</title><title>Journal of endocrinological investigation</title><addtitle>J Endocrinol Invest</addtitle><description>Objective
: To observe the effects of simvastatin on urinary excretion of matrix metalloproteinase-9 (MMP-9), renal expression of MMP-9, and investigate its possible renoprotective mechanisms in streptozotocin (STZ)-induced diabetic rats.
Method
: Twenty-four Wistar rats were divided into 3 groups: control healthy rats (group C, no.=8), untreated diabetic rats (group D, no.=8), and diabetic rats treated with simvastatin (20 mg/kg/d) (group S, no.=8). Peripheral blood glucose was tested weekly, glycosylated hemoglobin A
1c
(HbA
1c
), total cholesterol (TC), LDL cholesterol (LDL-C) levels, and urinary albumin (ALB) excretion rate as well as the urinary excretion rates of retinol-binding protein (RBP) and MMP-9 were tested at 8
th
week. The renal tissues of diabetic rats were obtained for evaluating kidney/body weight ratio, observing renal pathological changes by electron microscope and examining the expression of renal MMP-9 mRNA by RT-PCR.
Results
: There was no statistical difference on the change of peripheral blood TC and LDL-C between group C and group D. Peripheral blood glucose, HbA
1c
levels kidney/body weight ratio urinary excretion rates of ALB, RBP, and MMP-9 concurrently with the expression of renal MMP-9 mRNA were significantly higher in groups D and S compared with group C (
p
<0.01). Treatment with simvastatin significantly lowered peripheral blood TC, LDL-C, kidney/body weight ratio, urinary excretion rates of ALB, RBP, and MMP-9 as well as the expression of renal MMP-9 mRNA (
p
<0.01); however, there was no evident effect on the change of blood glucose and HbA
1c
levels between group D and group S. In addition, urinary excretion rate of MMP-9 showed positive correlations with the urinary ALB excretion and urinary RBP excretion. Pathological lesions of the glomeruli and epithelial cells foot processes (FP) was lightened by simvastatin.
Conclusion
: Simvastatin may has a potential therapeutic target in diabetic nephropathy, which may be partly attributed to down-regulating over-expression of MMP-9 in renal tissue.</description><subject>Endocrinology</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Metabolic Diseases</subject><subject>Original Articles</subject><issn>0391-4097</issn><issn>1720-8386</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><recordid>eNpt0EFLwzAYBuAgCs7pxV-Qs1JN-jVLctThVBh4UM8lTdIts01Lksp28q9bneLF08sHDy98L0LnlFxRQvj17YIAFDMmxAGaUJ6TTICYHaIJAUmzgkh-jE5i3BACHASfoI9n176rmFRyHvehS1aniI1TlU1O46DGS62U8zHhN2e83WHnN0PY4bQO3bBaj2lxHPo-2Bhd53FX42C9anCrUnBb3Nqkmqb77nZeRZtJbLe__BQd1aqJ9uwnp-h1cfcyf8iWT_eP85tlpqnkKROMaDCm4sLMqAADRkEtNWNgZSWp4qYQhRCcgdZFYaGSxOaUG84IyyGvYYou9r06dDEGW5d9cK0Ku5KS8mu68m-6EV_ucRyRX9lQbrohjD_F__QnvuVyGA</recordid><startdate>20100501</startdate><enddate>20100501</enddate><creator>Yao, X. M.</creator><creator>Ye, S. D.</creator><creator>Zai, Z.</creator><creator>Chen, Y.</creator><creator>Li, X. C.</creator><creator>Yang, G. W.</creator><creator>Wang, Y. X.</creator><creator>Chen, K.</creator><general>Springer International Publishing</general><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>20100501</creationdate><title>Simvastatin protects diabetic rats against kidney injury through the suppression of renal matrix metalloproteinase-9 expression</title><author>Yao, X. M. ; Ye, S. D. ; Zai, Z. ; Chen, Y. ; Li, X. C. ; Yang, G. W. ; Wang, Y. X. ; Chen, K.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c197t-850c3ddb78d6183d3da3f9c553e9b91a7d48488753cc44e3b90e217d7505232f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Endocrinology</topic><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>Metabolic Diseases</topic><topic>Original Articles</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yao, X. M.</creatorcontrib><creatorcontrib>Ye, S. D.</creatorcontrib><creatorcontrib>Zai, Z.</creatorcontrib><creatorcontrib>Chen, Y.</creatorcontrib><creatorcontrib>Li, X. C.</creatorcontrib><creatorcontrib>Yang, G. W.</creatorcontrib><creatorcontrib>Wang, Y. X.</creatorcontrib><creatorcontrib>Chen, K.</creatorcontrib><collection>CrossRef</collection><jtitle>Journal of endocrinological investigation</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yao, X. M.</au><au>Ye, S. D.</au><au>Zai, Z.</au><au>Chen, Y.</au><au>Li, X. C.</au><au>Yang, G. W.</au><au>Wang, Y. X.</au><au>Chen, K.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Simvastatin protects diabetic rats against kidney injury through the suppression of renal matrix metalloproteinase-9 expression</atitle><jtitle>Journal of endocrinological investigation</jtitle><stitle>J Endocrinol Invest</stitle><date>2010-05-01</date><risdate>2010</risdate><volume>33</volume><issue>5</issue><spage>292</spage><epage>296</epage><pages>292-296</pages><issn>0391-4097</issn><eissn>1720-8386</eissn><abstract>Objective
: To observe the effects of simvastatin on urinary excretion of matrix metalloproteinase-9 (MMP-9), renal expression of MMP-9, and investigate its possible renoprotective mechanisms in streptozotocin (STZ)-induced diabetic rats.
Method
: Twenty-four Wistar rats were divided into 3 groups: control healthy rats (group C, no.=8), untreated diabetic rats (group D, no.=8), and diabetic rats treated with simvastatin (20 mg/kg/d) (group S, no.=8). Peripheral blood glucose was tested weekly, glycosylated hemoglobin A
1c
(HbA
1c
), total cholesterol (TC), LDL cholesterol (LDL-C) levels, and urinary albumin (ALB) excretion rate as well as the urinary excretion rates of retinol-binding protein (RBP) and MMP-9 were tested at 8
th
week. The renal tissues of diabetic rats were obtained for evaluating kidney/body weight ratio, observing renal pathological changes by electron microscope and examining the expression of renal MMP-9 mRNA by RT-PCR.
Results
: There was no statistical difference on the change of peripheral blood TC and LDL-C between group C and group D. Peripheral blood glucose, HbA
1c
levels kidney/body weight ratio urinary excretion rates of ALB, RBP, and MMP-9 concurrently with the expression of renal MMP-9 mRNA were significantly higher in groups D and S compared with group C (
p
<0.01). Treatment with simvastatin significantly lowered peripheral blood TC, LDL-C, kidney/body weight ratio, urinary excretion rates of ALB, RBP, and MMP-9 as well as the expression of renal MMP-9 mRNA (
p
<0.01); however, there was no evident effect on the change of blood glucose and HbA
1c
levels between group D and group S. In addition, urinary excretion rate of MMP-9 showed positive correlations with the urinary ALB excretion and urinary RBP excretion. Pathological lesions of the glomeruli and epithelial cells foot processes (FP) was lightened by simvastatin.
Conclusion
: Simvastatin may has a potential therapeutic target in diabetic nephropathy, which may be partly attributed to down-regulating over-expression of MMP-9 in renal tissue.</abstract><cop>Cham</cop><pub>Springer International Publishing</pub><doi>10.1007/BF03346588</doi><tpages>5</tpages></addata></record> |
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| subjects | Endocrinology Medicine Medicine & Public Health Metabolic Diseases Original Articles |
| title | Simvastatin protects diabetic rats against kidney injury through the suppression of renal matrix metalloproteinase-9 expression |
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