Callus Culture and Plant Regeneration from Seedling Explants of Pergularia daemia (Forsk) Chiov
Callus cultures were established from seedling explants of Pergularia daemia (Forsk) Chiov on Murashige and Skoog (MS) medium supplemented with different concentrations of auxins. Optimal callus developed from leaf explants on MS medium supplemented with 2,4-D (2 mg l⁻¹) + 2iP (0.1 mg l⁻¹), was used...
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| Veröffentlicht in: | Journal of plant biochemistry and biotechnology 2008, Vol.17 (1), p.99-101 |
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| container_title | Journal of plant biochemistry and biotechnology |
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| creator | Kiranmai, C Aruna, V Karuppusamy, S Pullaiah, T |
| description | Callus cultures were established from seedling explants of Pergularia daemia (Forsk) Chiov on Murashige and Skoog (MS) medium supplemented with different concentrations of auxins. Optimal callus developed from leaf explants on MS medium supplemented with 2,4-D (2 mg l⁻¹) + 2iP (0.1 mg l⁻¹), was used for morphogenesis. Adventitious shoots were regenerated (70%) from the calli on MS medium supplemented with NAA (0.1 mg l⁻¹)+ BAP (2 mg l⁻¹). Individual shoots were rooted on half strength MS medium supplemented with 0.1 mg l⁻¹ IBA. Plantlets with well developed roots were successfully transferred to soil and 50% of the transferred plants survived. |
| doi_str_mv | 10.1007/BF03263269 |
| format | Article |
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Optimal callus developed from leaf explants on MS medium supplemented with 2,4-D (2 mg l⁻¹) + 2iP (0.1 mg l⁻¹), was used for morphogenesis. Adventitious shoots were regenerated (70%) from the calli on MS medium supplemented with NAA (0.1 mg l⁻¹)+ BAP (2 mg l⁻¹). Individual shoots were rooted on half strength MS medium supplemented with 0.1 mg l⁻¹ IBA. Plantlets with well developed roots were successfully transferred to soil and 50% of the transferred plants survived.</description><identifier>ISSN: 0971-7811</identifier><identifier>EISSN: 0974-1275</identifier><identifier>DOI: 10.1007/BF03263269</identifier><language>eng</language><publisher>New Delhi: Springer-Verlag</publisher><subject>2,4-D ; adventitious shoots ; Biomedical and Life Sciences ; callus ; callus culture ; Cell Biology ; explants ; indole butyric acid ; leaves ; Life Sciences ; naphthaleneacetic acid ; Plant Biochemistry ; plant cultural practices ; plantlets ; Protein Science ; Receptors ; roots ; seedlings ; Short Communication</subject><ispartof>Journal of plant biochemistry and biotechnology, 2008, Vol.17 (1), p.99-101</ispartof><rights>Springer-Verlag 2008</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c291t-176fabffec67b761e8e2d0ca38ad78d94c7002729af072bef127760a79ed0a213</citedby><cites>FETCH-LOGICAL-c291t-176fabffec67b761e8e2d0ca38ad78d94c7002729af072bef127760a79ed0a213</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/BF03263269$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/BF03263269$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,4009,27902,27903,27904,41467,42536,51297</link.rule.ids></links><search><creatorcontrib>Kiranmai, C</creatorcontrib><creatorcontrib>Aruna, V</creatorcontrib><creatorcontrib>Karuppusamy, S</creatorcontrib><creatorcontrib>Pullaiah, T</creatorcontrib><title>Callus Culture and Plant Regeneration from Seedling Explants of Pergularia daemia (Forsk) Chiov</title><title>Journal of plant biochemistry and biotechnology</title><addtitle>J. Plant Biochem. Biotechnol</addtitle><description>Callus cultures were established from seedling explants of Pergularia daemia (Forsk) Chiov on Murashige and Skoog (MS) medium supplemented with different concentrations of auxins. Optimal callus developed from leaf explants on MS medium supplemented with 2,4-D (2 mg l⁻¹) + 2iP (0.1 mg l⁻¹), was used for morphogenesis. Adventitious shoots were regenerated (70%) from the calli on MS medium supplemented with NAA (0.1 mg l⁻¹)+ BAP (2 mg l⁻¹). Individual shoots were rooted on half strength MS medium supplemented with 0.1 mg l⁻¹ IBA. Plantlets with well developed roots were successfully transferred to soil and 50% of the transferred plants survived.</description><subject>2,4-D</subject><subject>adventitious shoots</subject><subject>Biomedical and Life Sciences</subject><subject>callus</subject><subject>callus culture</subject><subject>Cell Biology</subject><subject>explants</subject><subject>indole butyric acid</subject><subject>leaves</subject><subject>Life Sciences</subject><subject>naphthaleneacetic acid</subject><subject>Plant Biochemistry</subject><subject>plant cultural practices</subject><subject>plantlets</subject><subject>Protein Science</subject><subject>Receptors</subject><subject>roots</subject><subject>seedlings</subject><subject>Short Communication</subject><issn>0971-7811</issn><issn>0974-1275</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><recordid>eNptkE9Lw0AQxRdRsFYvfgH36B-is5s0mxxtaFUoWKw9L9NkNqam2bKbiH57Uyt4cRh4c_jxmPcYOxdwKwDU3XgKoYz7TQ_YAFIVBUKq0eHPLQKVCHHMTrxfA0QjBdGA6QzruvM86-q2c8SxKfi8xqblL1RSQw7byjbcOLvhC6KirpqSTz63O8Rza_icXNnV6CrkBdKml8updf79imdvlf04ZUcGa09nvzpky-nkNXsMZs8PT9n9LMhlKtpAqNjgyhjKY7VSsaCEZAE5hgkWKinSKFcAUskUDSi5ItPHUjGgSqkAlCIcsuu9b-6s946M3rpqg-5LC9C7avRfNT18s4d9DzUlOb22nWv6__6nL_a0QauxdJXXy4UEEUE_URqF4Te8jG0r</recordid><startdate>2008</startdate><enddate>2008</enddate><creator>Kiranmai, C</creator><creator>Aruna, V</creator><creator>Karuppusamy, S</creator><creator>Pullaiah, T</creator><general>Springer-Verlag</general><general>Springer India</general><scope>FBQ</scope><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>2008</creationdate><title>Callus Culture and Plant Regeneration from Seedling Explants of Pergularia daemia (Forsk) Chiov</title><author>Kiranmai, C ; Aruna, V ; Karuppusamy, S ; Pullaiah, T</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c291t-176fabffec67b761e8e2d0ca38ad78d94c7002729af072bef127760a79ed0a213</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>2,4-D</topic><topic>adventitious shoots</topic><topic>Biomedical and Life Sciences</topic><topic>callus</topic><topic>callus culture</topic><topic>Cell Biology</topic><topic>explants</topic><topic>indole butyric acid</topic><topic>leaves</topic><topic>Life Sciences</topic><topic>naphthaleneacetic acid</topic><topic>Plant Biochemistry</topic><topic>plant cultural practices</topic><topic>plantlets</topic><topic>Protein Science</topic><topic>Receptors</topic><topic>roots</topic><topic>seedlings</topic><topic>Short Communication</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kiranmai, C</creatorcontrib><creatorcontrib>Aruna, V</creatorcontrib><creatorcontrib>Karuppusamy, S</creatorcontrib><creatorcontrib>Pullaiah, T</creatorcontrib><collection>AGRIS</collection><collection>CrossRef</collection><jtitle>Journal of plant biochemistry and biotechnology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kiranmai, C</au><au>Aruna, V</au><au>Karuppusamy, S</au><au>Pullaiah, T</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Callus Culture and Plant Regeneration from Seedling Explants of Pergularia daemia (Forsk) Chiov</atitle><jtitle>Journal of plant biochemistry and biotechnology</jtitle><stitle>J. Plant Biochem. Biotechnol</stitle><date>2008</date><risdate>2008</risdate><volume>17</volume><issue>1</issue><spage>99</spage><epage>101</epage><pages>99-101</pages><issn>0971-7811</issn><eissn>0974-1275</eissn><abstract>Callus cultures were established from seedling explants of Pergularia daemia (Forsk) Chiov on Murashige and Skoog (MS) medium supplemented with different concentrations of auxins. Optimal callus developed from leaf explants on MS medium supplemented with 2,4-D (2 mg l⁻¹) + 2iP (0.1 mg l⁻¹), was used for morphogenesis. Adventitious shoots were regenerated (70%) from the calli on MS medium supplemented with NAA (0.1 mg l⁻¹)+ BAP (2 mg l⁻¹). Individual shoots were rooted on half strength MS medium supplemented with 0.1 mg l⁻¹ IBA. Plantlets with well developed roots were successfully transferred to soil and 50% of the transferred plants survived.</abstract><cop>New Delhi</cop><pub>Springer-Verlag</pub><doi>10.1007/BF03263269</doi><tpages>3</tpages></addata></record> |
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| ispartof | Journal of plant biochemistry and biotechnology, 2008, Vol.17 (1), p.99-101 |
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| source | Springer Nature - Complete Springer Journals |
| subjects | 2,4-D adventitious shoots Biomedical and Life Sciences callus callus culture Cell Biology explants indole butyric acid leaves Life Sciences naphthaleneacetic acid Plant Biochemistry plant cultural practices plantlets Protein Science Receptors roots seedlings Short Communication |
| title | Callus Culture and Plant Regeneration from Seedling Explants of Pergularia daemia (Forsk) Chiov |
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