Organogenesis in female gametophyte and embryo cultures of cycads: Cycas circinalis and Zamia integrifolia

Female gametophyte of Cycas circinalis and Zamia integrifolia and embryo of C. circinalis were cultured in vitro on MS medium supplemented with various auxins and cytokinins. In female gametophyte cultures of C. circinalis and Z. integrifolia, auxins (NAA, 2, 4-D) alone resulted in callus formation. However, when auxin was used in combination with BAP or Kn, nodulation was pobserved. Embryos of C. circinalis grown on a medium containing 2, 4-D and BAP showed organogenic responce and bulbil-like structures.