Molecular profiling of non-genotoxic hepatocarcinogenesis using differential display reverse transcription-polymerase chain reaction (ddRT-PCR)
The technique of differential display reverse transcription-polymerase chain reaction (ddRT-PCR) has been used to produce unique profiles of up-regulated and down-regulated gene expression in the liver of male Wistar rats following short term exposure to the non-genotoxic hepatocarcinogens, phenobar...
Gespeichert in:
| Veröffentlicht in: | European journal of drug metabolism and pharmacokinetics 1997-10, Vol.22 (4), p.329-333 |
|---|---|
| Hauptverfasser: | , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 333 |
|---|---|
| container_issue | 4 |
| container_start_page | 329 |
| container_title | European journal of drug metabolism and pharmacokinetics |
| container_volume | 22 |
| creator | Rockett, J C Esdaile, D J Gibson, G G |
| description | The technique of differential display reverse transcription-polymerase chain reaction (ddRT-PCR) has been used to produce unique profiles of up-regulated and down-regulated gene expression in the liver of male Wistar rats following short term exposure to the non-genotoxic hepatocarcinogens, phenobarbital and WY-14,643. Animals were treated for 3 days, whereupon their livers were extracted and snap frozen. mRNA was prepared from the livers and used for ddRT-PCR. Individual bands from the differential displays were extracted and cloned. False positives were eliminated by dotblot screening and true positives then sequenced and identified. |
| doi_str_mv | 10.1007/BF03190966 |
| format | Article |
| fullrecord | <record><control><sourceid>pubmed_cross</sourceid><recordid>TN_cdi_crossref_primary_10_1007_BF03190966</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>9512930</sourcerecordid><originalsourceid>FETCH-LOGICAL-c282t-e306aaac4aaa7ef0937a44fe3ac00b15caf7e580bdd7390a8a043963c8107a353</originalsourceid><addsrcrecordid>eNpFkEFLw0AQhRdRaqm9eBdyVCE6ySbZ5KjFqlBRSj2H6Wa2XUl3w24q9lf4l91S0TnMMO99vMNj7DyBmwRA3N5PgScVVEVxxIZpAiKGpIRjNgQuylgE_ZSNvf-AMLys8rwYsEGVJ2nFYci-X2xLctuiizpnlW61WUVWRcaaeEXG9vZLy2hNHfZWopPa2CCT1z7a-j3baKXIkek1tuHxXYu7yNEnOU9R79B46XTX65DX2Xa3IYfBkGvUJmAo90502TTzRfw2mV-dsROFrafx7x2x9-nDYvIUz14fnyd3s1imZdrHxKFARJmFJUhBxQVmmSKOEmCZ5BKVoLyEZdMIXgGWCBmvCi7LUBDynI_Y9SFXOuu9I1V3Tm_Q7eoE6n2v9X-vAb44wN12uaHmD_1tkf8AFcR2aw</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>Molecular profiling of non-genotoxic hepatocarcinogenesis using differential display reverse transcription-polymerase chain reaction (ddRT-PCR)</title><source>MEDLINE</source><source>SpringerLink Journals - AutoHoldings</source><creator>Rockett, J C ; Esdaile, D J ; Gibson, G G</creator><creatorcontrib>Rockett, J C ; Esdaile, D J ; Gibson, G G</creatorcontrib><description>The technique of differential display reverse transcription-polymerase chain reaction (ddRT-PCR) has been used to produce unique profiles of up-regulated and down-regulated gene expression in the liver of male Wistar rats following short term exposure to the non-genotoxic hepatocarcinogens, phenobarbital and WY-14,643. Animals were treated for 3 days, whereupon their livers were extracted and snap frozen. mRNA was prepared from the livers and used for ddRT-PCR. Individual bands from the differential displays were extracted and cloned. False positives were eliminated by dotblot screening and true positives then sequenced and identified.</description><identifier>ISSN: 0378-7966</identifier><identifier>EISSN: 2107-0180</identifier><identifier>DOI: 10.1007/BF03190966</identifier><identifier>PMID: 9512930</identifier><language>eng</language><publisher>France</publisher><subject>Animals ; Carcinogens - toxicity ; DNA, Neoplasm - chemistry ; DNA, Neoplasm - genetics ; Liver Neoplasms, Experimental - chemically induced ; Liver Neoplasms, Experimental - genetics ; Male ; Models, Molecular ; Mutagens - toxicity ; Phenobarbital - toxicity ; Polymerase Chain Reaction ; Pyrimidines - toxicity ; Rats ; Rats, Wistar ; RNA, Messenger - biosynthesis</subject><ispartof>European journal of drug metabolism and pharmacokinetics, 1997-10, Vol.22 (4), p.329-333</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c282t-e306aaac4aaa7ef0937a44fe3ac00b15caf7e580bdd7390a8a043963c8107a353</citedby><cites>FETCH-LOGICAL-c282t-e306aaac4aaa7ef0937a44fe3ac00b15caf7e580bdd7390a8a043963c8107a353</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9512930$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Rockett, J C</creatorcontrib><creatorcontrib>Esdaile, D J</creatorcontrib><creatorcontrib>Gibson, G G</creatorcontrib><title>Molecular profiling of non-genotoxic hepatocarcinogenesis using differential display reverse transcription-polymerase chain reaction (ddRT-PCR)</title><title>European journal of drug metabolism and pharmacokinetics</title><addtitle>Eur J Drug Metab Pharmacokinet</addtitle><description>The technique of differential display reverse transcription-polymerase chain reaction (ddRT-PCR) has been used to produce unique profiles of up-regulated and down-regulated gene expression in the liver of male Wistar rats following short term exposure to the non-genotoxic hepatocarcinogens, phenobarbital and WY-14,643. Animals were treated for 3 days, whereupon their livers were extracted and snap frozen. mRNA was prepared from the livers and used for ddRT-PCR. Individual bands from the differential displays were extracted and cloned. False positives were eliminated by dotblot screening and true positives then sequenced and identified.</description><subject>Animals</subject><subject>Carcinogens - toxicity</subject><subject>DNA, Neoplasm - chemistry</subject><subject>DNA, Neoplasm - genetics</subject><subject>Liver Neoplasms, Experimental - chemically induced</subject><subject>Liver Neoplasms, Experimental - genetics</subject><subject>Male</subject><subject>Models, Molecular</subject><subject>Mutagens - toxicity</subject><subject>Phenobarbital - toxicity</subject><subject>Polymerase Chain Reaction</subject><subject>Pyrimidines - toxicity</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>RNA, Messenger - biosynthesis</subject><issn>0378-7966</issn><issn>2107-0180</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkEFLw0AQhRdRaqm9eBdyVCE6ySbZ5KjFqlBRSj2H6Wa2XUl3w24q9lf4l91S0TnMMO99vMNj7DyBmwRA3N5PgScVVEVxxIZpAiKGpIRjNgQuylgE_ZSNvf-AMLys8rwYsEGVJ2nFYci-X2xLctuiizpnlW61WUVWRcaaeEXG9vZLy2hNHfZWopPa2CCT1z7a-j3baKXIkek1tuHxXYu7yNEnOU9R79B46XTX65DX2Xa3IYfBkGvUJmAo90502TTzRfw2mV-dsROFrafx7x2x9-nDYvIUz14fnyd3s1imZdrHxKFARJmFJUhBxQVmmSKOEmCZ5BKVoLyEZdMIXgGWCBmvCi7LUBDynI_Y9SFXOuu9I1V3Tm_Q7eoE6n2v9X-vAb44wN12uaHmD_1tkf8AFcR2aw</recordid><startdate>19971001</startdate><enddate>19971001</enddate><creator>Rockett, J C</creator><creator>Esdaile, D J</creator><creator>Gibson, G G</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>19971001</creationdate><title>Molecular profiling of non-genotoxic hepatocarcinogenesis using differential display reverse transcription-polymerase chain reaction (ddRT-PCR)</title><author>Rockett, J C ; Esdaile, D J ; Gibson, G G</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c282t-e306aaac4aaa7ef0937a44fe3ac00b15caf7e580bdd7390a8a043963c8107a353</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>Animals</topic><topic>Carcinogens - toxicity</topic><topic>DNA, Neoplasm - chemistry</topic><topic>DNA, Neoplasm - genetics</topic><topic>Liver Neoplasms, Experimental - chemically induced</topic><topic>Liver Neoplasms, Experimental - genetics</topic><topic>Male</topic><topic>Models, Molecular</topic><topic>Mutagens - toxicity</topic><topic>Phenobarbital - toxicity</topic><topic>Polymerase Chain Reaction</topic><topic>Pyrimidines - toxicity</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>RNA, Messenger - biosynthesis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Rockett, J C</creatorcontrib><creatorcontrib>Esdaile, D J</creatorcontrib><creatorcontrib>Gibson, G G</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><jtitle>European journal of drug metabolism and pharmacokinetics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Rockett, J C</au><au>Esdaile, D J</au><au>Gibson, G G</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Molecular profiling of non-genotoxic hepatocarcinogenesis using differential display reverse transcription-polymerase chain reaction (ddRT-PCR)</atitle><jtitle>European journal of drug metabolism and pharmacokinetics</jtitle><addtitle>Eur J Drug Metab Pharmacokinet</addtitle><date>1997-10-01</date><risdate>1997</risdate><volume>22</volume><issue>4</issue><spage>329</spage><epage>333</epage><pages>329-333</pages><issn>0378-7966</issn><eissn>2107-0180</eissn><abstract>The technique of differential display reverse transcription-polymerase chain reaction (ddRT-PCR) has been used to produce unique profiles of up-regulated and down-regulated gene expression in the liver of male Wistar rats following short term exposure to the non-genotoxic hepatocarcinogens, phenobarbital and WY-14,643. Animals were treated for 3 days, whereupon their livers were extracted and snap frozen. mRNA was prepared from the livers and used for ddRT-PCR. Individual bands from the differential displays were extracted and cloned. False positives were eliminated by dotblot screening and true positives then sequenced and identified.</abstract><cop>France</cop><pmid>9512930</pmid><doi>10.1007/BF03190966</doi><tpages>5</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0378-7966 |
| ispartof | European journal of drug metabolism and pharmacokinetics, 1997-10, Vol.22 (4), p.329-333 |
| issn | 0378-7966 2107-0180 |
| language | eng |
| recordid | cdi_crossref_primary_10_1007_BF03190966 |
| source | MEDLINE; SpringerLink Journals - AutoHoldings |
| subjects | Animals Carcinogens - toxicity DNA, Neoplasm - chemistry DNA, Neoplasm - genetics Liver Neoplasms, Experimental - chemically induced Liver Neoplasms, Experimental - genetics Male Models, Molecular Mutagens - toxicity Phenobarbital - toxicity Polymerase Chain Reaction Pyrimidines - toxicity Rats Rats, Wistar RNA, Messenger - biosynthesis |
| title | Molecular profiling of non-genotoxic hepatocarcinogenesis using differential display reverse transcription-polymerase chain reaction (ddRT-PCR) |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-03T18%3A25%3A42IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-pubmed_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Molecular%20profiling%20of%20non-genotoxic%20hepatocarcinogenesis%20using%20differential%20display%20reverse%20transcription-polymerase%20chain%20reaction%20(ddRT-PCR)&rft.jtitle=European%20journal%20of%20drug%20metabolism%20and%20pharmacokinetics&rft.au=Rockett,%20J%20C&rft.date=1997-10-01&rft.volume=22&rft.issue=4&rft.spage=329&rft.epage=333&rft.pages=329-333&rft.issn=0378-7966&rft.eissn=2107-0180&rft_id=info:doi/10.1007/BF03190966&rft_dat=%3Cpubmed_cross%3E9512930%3C/pubmed_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/9512930&rfr_iscdi=true |