Conversion of lignocellulosics pretreated with liquid hot water to ethanol
Lignocellulosic materials pretreated using liquid hot water (LHW)(220 degrees C, 5 MPa, 120s) were fermented to ethanol by batch simultaneous saccharification and fermentation (SSF) using Saccharomyces cerevisiae in the presence of Trichoderma reesei cellulase. SSF of sugarcane bagasse (as received)...
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| Veröffentlicht in: | Applied Biochemistry and Biotechnology 1996-03, Vol.57 (1), p.157-170 |
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| container_title | Applied Biochemistry and Biotechnology |
| container_volume | 57 |
| creator | Van Walsum, G.P. (Dartmouth College, Hanover, NH.) Allen, S.G Spencer, M.J Laser, M.S Antal, M.J. Jr Lynd, L.R |
| description | Lignocellulosic materials pretreated using liquid hot water (LHW)(220 degrees C, 5 MPa, 120s) were fermented to ethanol by batch simultaneous saccharification and fermentation (SSF) using Saccharomyces cerevisiae in the presence of Trichoderma reesei cellulase. SSF of sugarcane bagasse (as received), aspen chips (smallest dimension 3 mm), and mixed hardwood flour (-60 +70 mesh) resulted in 90% conversion to ethanol in 2-5 d at enzyme loadings of 15-30 FPU/g. In most cases, 90% of the final conversion was achieved within 75 h of inoculation. Comminution of the pretreated substrates did not affect the conversion to ethanol. The hydrolysate produced from the LHW pretreatment showed slight inhibition of batch growth of S. cerevisiae. Solids pretreated at a concentration of 100 g/L were as reactive as those pretreated at a lower concentration, provided that the temperature was maintained at 220 degrees C |
| doi_str_mv | 10.1007/BF02941696 |
| format | Article |
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(Dartmouth College, Hanover, NH.) ; Allen, S.G ; Spencer, M.J ; Laser, M.S ; Antal, M.J. Jr ; Lynd, L.R</creator><creatorcontrib>Van Walsum, G.P. (Dartmouth College, Hanover, NH.) ; Allen, S.G ; Spencer, M.J ; Laser, M.S ; Antal, M.J. Jr ; Lynd, L.R</creatorcontrib><description>Lignocellulosic materials pretreated using liquid hot water (LHW)(220 degrees C, 5 MPa, 120s) were fermented to ethanol by batch simultaneous saccharification and fermentation (SSF) using Saccharomyces cerevisiae in the presence of Trichoderma reesei cellulase. SSF of sugarcane bagasse (as received), aspen chips (smallest dimension 3 mm), and mixed hardwood flour (-60 +70 mesh) resulted in 90% conversion to ethanol in 2-5 d at enzyme loadings of 15-30 FPU/g. In most cases, 90% of the final conversion was achieved within 75 h of inoculation. Comminution of the pretreated substrates did not affect the conversion to ethanol. The hydrolysate produced from the LHW pretreatment showed slight inhibition of batch growth of S. cerevisiae. Solids pretreated at a concentration of 100 g/L were as reactive as those pretreated at a lower concentration, provided that the temperature was maintained at 220 degrees C</description><identifier>ISSN: 0273-2289</identifier><identifier>EISSN: 1559-0291</identifier><identifier>DOI: 10.1007/BF02941696</identifier><identifier>CODEN: ABIBDL</identifier><language>eng</language><publisher>Heidelberg: Springer</publisher><subject>09 BIOMASS FUELS ; BAGASSE ; BATCH CULTURE ; BIOCONVERSION ; Biofuel production ; Biological and medical sciences ; BIOLOGY AND MEDICINE, BASIC STUDIES ; BIOMASS ; Biotechnology ; CELLULOSE ; COMMINUTION ; CONCENTRATION RATIO ; COST ; EFFICIENCY ; Energy ; ENZYMATIC HYDROLYSIS ; ETANOL ; ETHANOL ; ETHANOL FUELS ; FERMENTACION ; FERMENTATION ; Fundamental and applied biological sciences. Psychology ; HOT WATER ; Industrial applications and implications. Economical aspects ; INHIBITION ; INOCULATION ; LIGNOCELLULOSE ; LIGNOCELULOSA ; PARTICLE SIZE ; REDUCTION ; RENEWABLE ENERGY SOURCES ; SACCHARIFICATION ; SACCHAROMYCES CEREVISIAE ; SUBSTRATES ; TEMPERATURE DEPENDENCE ; TRICHODERMA</subject><ispartof>Applied Biochemistry and Biotechnology, 1996-03, Vol.57 (1), p.157-170</ispartof><rights>1996 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c305t-9b106d8fa620215d3f7045b2fe3c8f301e0ec1fdd5f14f3a96db110cdfcf8c353</citedby><cites>FETCH-LOGICAL-c305t-9b106d8fa620215d3f7045b2fe3c8f301e0ec1fdd5f14f3a96db110cdfcf8c353</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>309,310,314,776,780,785,786,881,23909,23910,25118,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3165232$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.osti.gov/biblio/598205$$D View this record in Osti.gov$$Hfree_for_read</backlink></links><search><creatorcontrib>Van Walsum, G.P. (Dartmouth College, Hanover, NH.)</creatorcontrib><creatorcontrib>Allen, S.G</creatorcontrib><creatorcontrib>Spencer, M.J</creatorcontrib><creatorcontrib>Laser, M.S</creatorcontrib><creatorcontrib>Antal, M.J. Jr</creatorcontrib><creatorcontrib>Lynd, L.R</creatorcontrib><title>Conversion of lignocellulosics pretreated with liquid hot water to ethanol</title><title>Applied Biochemistry and Biotechnology</title><description>Lignocellulosic materials pretreated using liquid hot water (LHW)(220 degrees C, 5 MPa, 120s) were fermented to ethanol by batch simultaneous saccharification and fermentation (SSF) using Saccharomyces cerevisiae in the presence of Trichoderma reesei cellulase. SSF of sugarcane bagasse (as received), aspen chips (smallest dimension 3 mm), and mixed hardwood flour (-60 +70 mesh) resulted in 90% conversion to ethanol in 2-5 d at enzyme loadings of 15-30 FPU/g. In most cases, 90% of the final conversion was achieved within 75 h of inoculation. Comminution of the pretreated substrates did not affect the conversion to ethanol. The hydrolysate produced from the LHW pretreatment showed slight inhibition of batch growth of S. cerevisiae. Solids pretreated at a concentration of 100 g/L were as reactive as those pretreated at a lower concentration, provided that the temperature was maintained at 220 degrees C</description><subject>09 BIOMASS FUELS</subject><subject>BAGASSE</subject><subject>BATCH CULTURE</subject><subject>BIOCONVERSION</subject><subject>Biofuel production</subject><subject>Biological and medical sciences</subject><subject>BIOLOGY AND MEDICINE, BASIC STUDIES</subject><subject>BIOMASS</subject><subject>Biotechnology</subject><subject>CELLULOSE</subject><subject>COMMINUTION</subject><subject>CONCENTRATION RATIO</subject><subject>COST</subject><subject>EFFICIENCY</subject><subject>Energy</subject><subject>ENZYMATIC HYDROLYSIS</subject><subject>ETANOL</subject><subject>ETHANOL</subject><subject>ETHANOL FUELS</subject><subject>FERMENTACION</subject><subject>FERMENTATION</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>HOT WATER</subject><subject>Industrial applications and implications. Economical aspects</subject><subject>INHIBITION</subject><subject>INOCULATION</subject><subject>LIGNOCELLULOSE</subject><subject>LIGNOCELULOSA</subject><subject>PARTICLE SIZE</subject><subject>REDUCTION</subject><subject>RENEWABLE ENERGY SOURCES</subject><subject>SACCHARIFICATION</subject><subject>SACCHAROMYCES CEREVISIAE</subject><subject>SUBSTRATES</subject><subject>TEMPERATURE DEPENDENCE</subject><subject>TRICHODERMA</subject><issn>0273-2289</issn><issn>1559-0291</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><recordid>eNpF0E1LAzEQBuAgCtbqxaOnCJ6E1Zmk2d0ctVg_KHjQnpc0H25k3dQktfjv3VLR0zDD887hJeQU4QoBquvbGTA5wVKWe2SEQshi2HGfjIBVvGCslofkKKV3AGS1qEbkaRr6LxuTDz0Njnb-rQ_adt26C8nrRFfR5mhVtoZufG4H8Ln2hrYh081wjTQHanOr-tAdkwOnumRPfueYLGZ3r9OHYv58_zi9mReag8iFXCKUpnaqZMBQGO4qmIglc5br2nFAC1ajM0Y4nDiuZGmWiKCN067WXPAxOd_9DSn7JmmfrW516HurcyNkzWBrLndGx5BStK5ZRf-h4neD0Gybav6bGvDFDq9U0qpzUfXap78Ex1IwzgZ2tmNOhUa9xYEsXmSFJUjGfwCz5XC4</recordid><startdate>19960301</startdate><enddate>19960301</enddate><creator>Van Walsum, G.P. (Dartmouth College, Hanover, NH.)</creator><creator>Allen, S.G</creator><creator>Spencer, M.J</creator><creator>Laser, M.S</creator><creator>Antal, M.J. Jr</creator><creator>Lynd, L.R</creator><general>Springer</general><scope>FBQ</scope><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>OTOTI</scope></search><sort><creationdate>19960301</creationdate><title>Conversion of lignocellulosics pretreated with liquid hot water to ethanol</title><author>Van Walsum, G.P. (Dartmouth College, Hanover, NH.) ; Allen, S.G ; Spencer, M.J ; Laser, M.S ; Antal, M.J. Jr ; Lynd, L.R</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c305t-9b106d8fa620215d3f7045b2fe3c8f301e0ec1fdd5f14f3a96db110cdfcf8c353</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1996</creationdate><topic>09 BIOMASS FUELS</topic><topic>BAGASSE</topic><topic>BATCH CULTURE</topic><topic>BIOCONVERSION</topic><topic>Biofuel production</topic><topic>Biological and medical sciences</topic><topic>BIOLOGY AND MEDICINE, BASIC STUDIES</topic><topic>BIOMASS</topic><topic>Biotechnology</topic><topic>CELLULOSE</topic><topic>COMMINUTION</topic><topic>CONCENTRATION RATIO</topic><topic>COST</topic><topic>EFFICIENCY</topic><topic>Energy</topic><topic>ENZYMATIC HYDROLYSIS</topic><topic>ETANOL</topic><topic>ETHANOL</topic><topic>ETHANOL FUELS</topic><topic>FERMENTACION</topic><topic>FERMENTATION</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>HOT WATER</topic><topic>Industrial applications and implications. Economical aspects</topic><topic>INHIBITION</topic><topic>INOCULATION</topic><topic>LIGNOCELLULOSE</topic><topic>LIGNOCELULOSA</topic><topic>PARTICLE SIZE</topic><topic>REDUCTION</topic><topic>RENEWABLE ENERGY SOURCES</topic><topic>SACCHARIFICATION</topic><topic>SACCHAROMYCES CEREVISIAE</topic><topic>SUBSTRATES</topic><topic>TEMPERATURE DEPENDENCE</topic><topic>TRICHODERMA</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Van Walsum, G.P. (Dartmouth College, Hanover, NH.)</creatorcontrib><creatorcontrib>Allen, S.G</creatorcontrib><creatorcontrib>Spencer, M.J</creatorcontrib><creatorcontrib>Laser, M.S</creatorcontrib><creatorcontrib>Antal, M.J. Jr</creatorcontrib><creatorcontrib>Lynd, L.R</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>OSTI.GOV</collection><jtitle>Applied Biochemistry and Biotechnology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Van Walsum, G.P. (Dartmouth College, Hanover, NH.)</au><au>Allen, S.G</au><au>Spencer, M.J</au><au>Laser, M.S</au><au>Antal, M.J. Jr</au><au>Lynd, L.R</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Conversion of lignocellulosics pretreated with liquid hot water to ethanol</atitle><jtitle>Applied Biochemistry and Biotechnology</jtitle><date>1996-03-01</date><risdate>1996</risdate><volume>57</volume><issue>1</issue><spage>157</spage><epage>170</epage><pages>157-170</pages><issn>0273-2289</issn><eissn>1559-0291</eissn><coden>ABIBDL</coden><abstract>Lignocellulosic materials pretreated using liquid hot water (LHW)(220 degrees C, 5 MPa, 120s) were fermented to ethanol by batch simultaneous saccharification and fermentation (SSF) using Saccharomyces cerevisiae in the presence of Trichoderma reesei cellulase. SSF of sugarcane bagasse (as received), aspen chips (smallest dimension 3 mm), and mixed hardwood flour (-60 +70 mesh) resulted in 90% conversion to ethanol in 2-5 d at enzyme loadings of 15-30 FPU/g. In most cases, 90% of the final conversion was achieved within 75 h of inoculation. Comminution of the pretreated substrates did not affect the conversion to ethanol. The hydrolysate produced from the LHW pretreatment showed slight inhibition of batch growth of S. cerevisiae. Solids pretreated at a concentration of 100 g/L were as reactive as those pretreated at a lower concentration, provided that the temperature was maintained at 220 degrees C</abstract><cop>Heidelberg</cop><pub>Springer</pub><doi>10.1007/BF02941696</doi><tpages>14</tpages></addata></record> |
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| ispartof | Applied Biochemistry and Biotechnology, 1996-03, Vol.57 (1), p.157-170 |
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| language | eng |
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| subjects | 09 BIOMASS FUELS BAGASSE BATCH CULTURE BIOCONVERSION Biofuel production Biological and medical sciences BIOLOGY AND MEDICINE, BASIC STUDIES BIOMASS Biotechnology CELLULOSE COMMINUTION CONCENTRATION RATIO COST EFFICIENCY Energy ENZYMATIC HYDROLYSIS ETANOL ETHANOL ETHANOL FUELS FERMENTACION FERMENTATION Fundamental and applied biological sciences. Psychology HOT WATER Industrial applications and implications. Economical aspects INHIBITION INOCULATION LIGNOCELLULOSE LIGNOCELULOSA PARTICLE SIZE REDUCTION RENEWABLE ENERGY SOURCES SACCHARIFICATION SACCHAROMYCES CEREVISIAE SUBSTRATES TEMPERATURE DEPENDENCE TRICHODERMA |
| title | Conversion of lignocellulosics pretreated with liquid hot water to ethanol |
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