Rapid and simple isolation of multigram goat IgG from serum using avid AL and radial flow column
Multigram quantities (2.5-10 g) of highly purified IgG were obtained within 4 h from serum by using Avid AL packed in a radial-flow column. Avid AL is an affinity gel containing a synthetic, low-mol-wt ligand capable of selectively binding IgG from serum of all animal species tested. By packing the...
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| Veröffentlicht in: | Applied biochemistry and biotechnology 1991-07, Vol.30 (1), p.111-119 |
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| container_title | Applied biochemistry and biotechnology |
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| creator | Ngo, T.T. (BioProbe International Inc., Tustin, CA) Khatter, N |
| description | Multigram quantities (2.5-10 g) of highly purified IgG were obtained within 4 h from serum by using Avid AL packed in a radial-flow column. Avid AL is an affinity gel containing a synthetic, low-mol-wt ligand capable of selectively binding IgG from serum of all animal species tested. By packing the gel in a radial-flow column up to 500 mL, a high flow rate of 50 mL/min can be achieved without adversely affecting the performance of the gel and the purity of the isolated antibody. |
| doi_str_mv | 10.1007/BF02922027 |
| format | Article |
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By packing the gel in a radial-flow column up to 500 mL, a high flow rate of 50 mL/min can be achieved without adversely affecting the performance of the gel and the purity of the isolated antibody.</description><identifier>ISSN: 0273-2289</identifier><identifier>EISSN: 1559-0291</identifier><identifier>DOI: 10.1007/BF02922027</identifier><identifier>PMID: 1952923</identifier><language>eng</language><publisher>United States</publisher><subject>AFFINITY GEL ; Animals ; CAPRIN ; CAPRINOS ; CHROMATOGRAPHIE ; CHROMATOGRAPHY ; Chromatography, Affinity - methods ; CROMATOGRAFIA ; Electrophoresis, Polyacrylamide Gel ; GEL FILTRATION CHROMATOGRAPHY ; GOATS ; Goats - blood ; Goats - immunology ; Humans ; Immunoglobulin G - isolation & purification ; IMMUNOGLOBULINE ; IMMUNOGLOBULINS ; INMUNOGLOBULINA ; LIGAND ; LIGANDOS ; LIGANDS ; Species Specificity ; SYNTHETIC LIGANDS</subject><ispartof>Applied biochemistry and biotechnology, 1991-07, Vol.30 (1), p.111-119</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c270t-f772bf9baf502586d1bc42d4e56e3b46b4ae5ec4cd153d9d7a8fcea8a881cd3</citedby><cites>FETCH-LOGICAL-c270t-f772bf9baf502586d1bc42d4e56e3b46b4ae5ec4cd153d9d7a8fcea8a881cd3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1952923$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ngo, T.T. (BioProbe International Inc., Tustin, CA)</creatorcontrib><creatorcontrib>Khatter, N</creatorcontrib><title>Rapid and simple isolation of multigram goat IgG from serum using avid AL and radial flow column</title><title>Applied biochemistry and biotechnology</title><addtitle>Appl Biochem Biotechnol</addtitle><description>Multigram quantities (2.5-10 g) of highly purified IgG were obtained within 4 h from serum by using Avid AL packed in a radial-flow column. Avid AL is an affinity gel containing a synthetic, low-mol-wt ligand capable of selectively binding IgG from serum of all animal species tested. 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(BioProbe International Inc., Tustin, CA) ; Khatter, N</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c270t-f772bf9baf502586d1bc42d4e56e3b46b4ae5ec4cd153d9d7a8fcea8a881cd3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1991</creationdate><topic>AFFINITY GEL</topic><topic>Animals</topic><topic>CAPRIN</topic><topic>CAPRINOS</topic><topic>CHROMATOGRAPHIE</topic><topic>CHROMATOGRAPHY</topic><topic>Chromatography, Affinity - methods</topic><topic>CROMATOGRAFIA</topic><topic>Electrophoresis, Polyacrylamide Gel</topic><topic>GEL FILTRATION CHROMATOGRAPHY</topic><topic>GOATS</topic><topic>Goats - blood</topic><topic>Goats - immunology</topic><topic>Humans</topic><topic>Immunoglobulin G - isolation & purification</topic><topic>IMMUNOGLOBULINE</topic><topic>IMMUNOGLOBULINS</topic><topic>INMUNOGLOBULINA</topic><topic>LIGAND</topic><topic>LIGANDOS</topic><topic>LIGANDS</topic><topic>Species Specificity</topic><topic>SYNTHETIC LIGANDS</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ngo, T.T. (BioProbe International Inc., Tustin, CA)</creatorcontrib><creatorcontrib>Khatter, N</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><jtitle>Applied biochemistry and biotechnology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ngo, T.T. (BioProbe International Inc., Tustin, CA)</au><au>Khatter, N</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Rapid and simple isolation of multigram goat IgG from serum using avid AL and radial flow column</atitle><jtitle>Applied biochemistry and biotechnology</jtitle><addtitle>Appl Biochem Biotechnol</addtitle><date>1991-07</date><risdate>1991</risdate><volume>30</volume><issue>1</issue><spage>111</spage><epage>119</epage><pages>111-119</pages><issn>0273-2289</issn><eissn>1559-0291</eissn><abstract>Multigram quantities (2.5-10 g) of highly purified IgG were obtained within 4 h from serum by using Avid AL packed in a radial-flow column. Avid AL is an affinity gel containing a synthetic, low-mol-wt ligand capable of selectively binding IgG from serum of all animal species tested. By packing the gel in a radial-flow column up to 500 mL, a high flow rate of 50 mL/min can be achieved without adversely affecting the performance of the gel and the purity of the isolated antibody.</abstract><cop>United States</cop><pmid>1952923</pmid><doi>10.1007/BF02922027</doi><tpages>9</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0273-2289 |
| ispartof | Applied biochemistry and biotechnology, 1991-07, Vol.30 (1), p.111-119 |
| issn | 0273-2289 1559-0291 |
| language | eng |
| recordid | cdi_crossref_primary_10_1007_BF02922027 |
| source | MEDLINE; SpringerLink Journals - AutoHoldings |
| subjects | AFFINITY GEL Animals CAPRIN CAPRINOS CHROMATOGRAPHIE CHROMATOGRAPHY Chromatography, Affinity - methods CROMATOGRAFIA Electrophoresis, Polyacrylamide Gel GEL FILTRATION CHROMATOGRAPHY GOATS Goats - blood Goats - immunology Humans Immunoglobulin G - isolation & purification IMMUNOGLOBULINE IMMUNOGLOBULINS INMUNOGLOBULINA LIGAND LIGANDOS LIGANDS Species Specificity SYNTHETIC LIGANDS |
| title | Rapid and simple isolation of multigram goat IgG from serum using avid AL and radial flow column |
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