Serial Cultivation of Epithelial Cells from Human and Macaque Salivary Glands
To study the regulation of human salivary-type gene expression we developed cell culture systems to support the growth and serial cultivation of salivary gland epithelial and fibroblastic cell types. We have established 22 independent salivary gland epithelial cell strains from parotid or submandibu...
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| Veröffentlicht in: | In Vitro Cellular & Developmental Biology - Animal 1991-12, Vol.27A (12), p.939-948 |
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| container_title | In Vitro Cellular & Developmental Biology - Animal |
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| creator | Sabatini, Linda M. Allen-Hoffmann, B. Lynn Thomas F. Warner Azen, Edwin A. |
| description | To study the regulation of human salivary-type gene expression we developed cell culture systems to support the growth and serial cultivation of salivary gland epithelial and fibroblastic cell types. We have established 22 independent salivary gland epithelial cell strains from parotid or submandibular glands of human or macaque origin. Nineteen strains were derived from normal tissues and three from human parotid gland tumors. Both the normal and the tumor-derived salivary gland epithelial cells could be serially cultivated with the aid of a 3T3 fibroblast feeder layer in a mixture of Ham's F12 and Dulbecco's modified Eagle's media supplemented with fetal bovine serum, calcium, cholera toxin, hydrocortisone, insulin, and epidermal growth factor. Salivary gland epithelial cells cultured under these conditions continued to express the genes for at least two acinar-cell-specific markers at early passages. Amylase enzyme activity was detected in conditioned media from cultured rhesus parotid epithelial cells as late as Passage 5. Proline-rich-protein-specific RNAs were detected in primary cultures of both rhesus and human parotid epithelial cells. Neither amylase enzyme activity nor PRP-specific RNAs were detected in fibroblasts isolated from the same tissues. In addition, salivary gland epithelial cells cultured under our conditions retain the capacity to undergo dramatic morphologic changes in response to different substrata. The cultured salivary gland epithelial cells we have established will be important tools for the study of salivary gland differentiation and the tissue-specific regulation of salivary-type gene expression. |
| doi_str_mv | 10.1007/bf02631121 |
| format | Article |
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Lynn ; Thomas F. Warner ; Azen, Edwin A.</creator><creatorcontrib>Sabatini, Linda M. ; Allen-Hoffmann, B. Lynn ; Thomas F. Warner ; Azen, Edwin A.</creatorcontrib><description>To study the regulation of human salivary-type gene expression we developed cell culture systems to support the growth and serial cultivation of salivary gland epithelial and fibroblastic cell types. We have established 22 independent salivary gland epithelial cell strains from parotid or submandibular glands of human or macaque origin. Nineteen strains were derived from normal tissues and three from human parotid gland tumors. Both the normal and the tumor-derived salivary gland epithelial cells could be serially cultivated with the aid of a 3T3 fibroblast feeder layer in a mixture of Ham's F12 and Dulbecco's modified Eagle's media supplemented with fetal bovine serum, calcium, cholera toxin, hydrocortisone, insulin, and epidermal growth factor. Salivary gland epithelial cells cultured under these conditions continued to express the genes for at least two acinar-cell-specific markers at early passages. Amylase enzyme activity was detected in conditioned media from cultured rhesus parotid epithelial cells as late as Passage 5. Proline-rich-protein-specific RNAs were detected in primary cultures of both rhesus and human parotid epithelial cells. Neither amylase enzyme activity nor PRP-specific RNAs were detected in fibroblasts isolated from the same tissues. In addition, salivary gland epithelial cells cultured under our conditions retain the capacity to undergo dramatic morphologic changes in response to different substrata. The cultured salivary gland epithelial cells we have established will be important tools for the study of salivary gland differentiation and the tissue-specific regulation of salivary-type gene expression.</description><identifier>ISSN: 0883-8364</identifier><identifier>EISSN: 2327-431X</identifier><identifier>EISSN: 1543-706X</identifier><identifier>DOI: 10.1007/bf02631121</identifier><identifier>PMID: 1721908</identifier><identifier>CODEN: ICDBEO</identifier><language>eng</language><publisher>Largo, MD: Tissue Culture Association, Inc</publisher><subject>Acinar cells ; Amylases - genetics ; Amylases - metabolism ; Animal cells ; Animals ; Biological and medical sciences ; Biotechnology ; Blotting, Northern ; Calcium - pharmacology ; Cell Division - drug effects ; Cell growth ; Cell lines ; Cells, Cultured ; Cholera Toxin - pharmacology ; Cultured cells ; Epidermal Growth Factor - pharmacology ; Epithelial Cells ; Epithelium - drug effects ; Epithelium - enzymology ; Establishment of new cell lines, improvement of cultural methods, mass cultures ; Eukaryotic cell cultures ; Feeder cells ; Fibroblasts ; Fundamental and applied biological sciences. Psychology ; Gene Expression Regulation, Enzymologic - drug effects ; Humans ; Hydrocortisone - pharmacology ; Insulin - pharmacology ; Macaca ; Methods. Procedures. Technologies ; Parotid gland ; Parotid Gland - cytology ; Parotid Gland - drug effects ; Parotid Gland - enzymology ; Peptides - genetics ; Peptides - metabolism ; Proline-Rich Protein Domains ; RNA ; RNA, Messenger - genetics ; RNA, Messenger - metabolism ; Salivary glands ; Salivary Glands - cytology ; Salivary Glands - drug effects ; Salivary Glands - enzymology ; Submandibular Gland - cytology ; Submandibular Gland - drug effects ; Submandibular Gland - enzymology ; Time Factors</subject><ispartof>In Vitro Cellular & Developmental Biology - Animal, 1991-12, Vol.27A (12), p.939-948</ispartof><rights>Copyright 1991 Tissue Culture Association</rights><rights>1992 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c398t-917354bacf1c2777513de2b512704ebc07a9c30a76c40543ec1d5501c7b54f023</citedby><cites>FETCH-LOGICAL-c398t-917354bacf1c2777513de2b512704ebc07a9c30a76c40543ec1d5501c7b54f023</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/4296781$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/4296781$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,778,782,801,27907,27908,58000,58233</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=5563662$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1721908$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sabatini, Linda M.</creatorcontrib><creatorcontrib>Allen-Hoffmann, B. Lynn</creatorcontrib><creatorcontrib>Thomas F. Warner</creatorcontrib><creatorcontrib>Azen, Edwin A.</creatorcontrib><title>Serial Cultivation of Epithelial Cells from Human and Macaque Salivary Glands</title><title>In Vitro Cellular & Developmental Biology - Animal</title><addtitle>In Vitro Cell Dev Biol</addtitle><description>To study the regulation of human salivary-type gene expression we developed cell culture systems to support the growth and serial cultivation of salivary gland epithelial and fibroblastic cell types. We have established 22 independent salivary gland epithelial cell strains from parotid or submandibular glands of human or macaque origin. Nineteen strains were derived from normal tissues and three from human parotid gland tumors. Both the normal and the tumor-derived salivary gland epithelial cells could be serially cultivated with the aid of a 3T3 fibroblast feeder layer in a mixture of Ham's F12 and Dulbecco's modified Eagle's media supplemented with fetal bovine serum, calcium, cholera toxin, hydrocortisone, insulin, and epidermal growth factor. Salivary gland epithelial cells cultured under these conditions continued to express the genes for at least two acinar-cell-specific markers at early passages. Amylase enzyme activity was detected in conditioned media from cultured rhesus parotid epithelial cells as late as Passage 5. Proline-rich-protein-specific RNAs were detected in primary cultures of both rhesus and human parotid epithelial cells. Neither amylase enzyme activity nor PRP-specific RNAs were detected in fibroblasts isolated from the same tissues. In addition, salivary gland epithelial cells cultured under our conditions retain the capacity to undergo dramatic morphologic changes in response to different substrata. The cultured salivary gland epithelial cells we have established will be important tools for the study of salivary gland differentiation and the tissue-specific regulation of salivary-type gene expression.</description><subject>Acinar cells</subject><subject>Amylases - genetics</subject><subject>Amylases - metabolism</subject><subject>Animal cells</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Blotting, Northern</subject><subject>Calcium - pharmacology</subject><subject>Cell Division - drug effects</subject><subject>Cell growth</subject><subject>Cell lines</subject><subject>Cells, Cultured</subject><subject>Cholera Toxin - pharmacology</subject><subject>Cultured cells</subject><subject>Epidermal Growth Factor - pharmacology</subject><subject>Epithelial Cells</subject><subject>Epithelium - drug effects</subject><subject>Epithelium - enzymology</subject><subject>Establishment of new cell lines, improvement of cultural methods, mass cultures</subject><subject>Eukaryotic cell cultures</subject><subject>Feeder cells</subject><subject>Fibroblasts</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression Regulation, Enzymologic - drug effects</subject><subject>Humans</subject><subject>Hydrocortisone - pharmacology</subject><subject>Insulin - pharmacology</subject><subject>Macaca</subject><subject>Methods. Procedures. Technologies</subject><subject>Parotid gland</subject><subject>Parotid Gland - cytology</subject><subject>Parotid Gland - drug effects</subject><subject>Parotid Gland - enzymology</subject><subject>Peptides - genetics</subject><subject>Peptides - metabolism</subject><subject>Proline-Rich Protein Domains</subject><subject>RNA</subject><subject>RNA, Messenger - genetics</subject><subject>RNA, Messenger - metabolism</subject><subject>Salivary glands</subject><subject>Salivary Glands - cytology</subject><subject>Salivary Glands - drug effects</subject><subject>Salivary Glands - enzymology</subject><subject>Submandibular Gland - cytology</subject><subject>Submandibular Gland - drug effects</subject><subject>Submandibular Gland - enzymology</subject><subject>Time Factors</subject><issn>0883-8364</issn><issn>2327-431X</issn><issn>1543-706X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1991</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkD1PwzAQhi0EKqWwMIPkgQkp4PNnMkLVD6RWDAWJLXIcR6RykmInSPx7UgJlsnTPc-e7F6FLIHdAiLrPCkIlA6BwhMaUURVxBm_HaEzimEUxk_wUnYWwJYQRSekIjUBRSEg8RuuN9aV2eNq5tvzUbdnUuCnwbFe279b9EOtcwIVvKrzsKl1jXed4rY3-6CzeaNd3-S-8cH05nKOTQrtgL37fCXqdz16my2j1vHiaPqwiw5K4jRJQTPBMmwIMVUoJYLmlmQCqCLeZIUonhhGtpOFEcGYN5EIQMCoTvL-VTdDtMNf4JgRvi3Tny6rfIwWS7iNJH-d_kfTy9SDvuqyy-b86ZNDzm1-ug9Gu8Lo2ZThoQkgm5f7Pq0HbhrbxB8xpIlUM7BtTQ3A_</recordid><startdate>19911201</startdate><enddate>19911201</enddate><creator>Sabatini, Linda M.</creator><creator>Allen-Hoffmann, B. Lynn</creator><creator>Thomas F. Warner</creator><creator>Azen, Edwin A.</creator><general>Tissue Culture Association, Inc</general><general>Society for In Vitro Biology</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>19911201</creationdate><title>Serial Cultivation of Epithelial Cells from Human and Macaque Salivary Glands</title><author>Sabatini, Linda M. ; Allen-Hoffmann, B. Lynn ; Thomas F. Warner ; Azen, Edwin A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c398t-917354bacf1c2777513de2b512704ebc07a9c30a76c40543ec1d5501c7b54f023</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1991</creationdate><topic>Acinar cells</topic><topic>Amylases - genetics</topic><topic>Amylases - metabolism</topic><topic>Animal cells</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>Blotting, Northern</topic><topic>Calcium - pharmacology</topic><topic>Cell Division - drug effects</topic><topic>Cell growth</topic><topic>Cell lines</topic><topic>Cells, Cultured</topic><topic>Cholera Toxin - pharmacology</topic><topic>Cultured cells</topic><topic>Epidermal Growth Factor - pharmacology</topic><topic>Epithelial Cells</topic><topic>Epithelium - drug effects</topic><topic>Epithelium - enzymology</topic><topic>Establishment of new cell lines, improvement of cultural methods, mass cultures</topic><topic>Eukaryotic cell cultures</topic><topic>Feeder cells</topic><topic>Fibroblasts</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression Regulation, Enzymologic - drug effects</topic><topic>Humans</topic><topic>Hydrocortisone - pharmacology</topic><topic>Insulin - pharmacology</topic><topic>Macaca</topic><topic>Methods. Procedures. Technologies</topic><topic>Parotid gland</topic><topic>Parotid Gland - cytology</topic><topic>Parotid Gland - drug effects</topic><topic>Parotid Gland - enzymology</topic><topic>Peptides - genetics</topic><topic>Peptides - metabolism</topic><topic>Proline-Rich Protein Domains</topic><topic>RNA</topic><topic>RNA, Messenger - genetics</topic><topic>RNA, Messenger - metabolism</topic><topic>Salivary glands</topic><topic>Salivary Glands - cytology</topic><topic>Salivary Glands - drug effects</topic><topic>Salivary Glands - enzymology</topic><topic>Submandibular Gland - cytology</topic><topic>Submandibular Gland - drug effects</topic><topic>Submandibular Gland - enzymology</topic><topic>Time Factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sabatini, Linda M.</creatorcontrib><creatorcontrib>Allen-Hoffmann, B. Lynn</creatorcontrib><creatorcontrib>Thomas F. Warner</creatorcontrib><creatorcontrib>Azen, Edwin A.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><jtitle>In Vitro Cellular & Developmental Biology - Animal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sabatini, Linda M.</au><au>Allen-Hoffmann, B. Lynn</au><au>Thomas F. Warner</au><au>Azen, Edwin A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Serial Cultivation of Epithelial Cells from Human and Macaque Salivary Glands</atitle><jtitle>In Vitro Cellular & Developmental Biology - Animal</jtitle><addtitle>In Vitro Cell Dev Biol</addtitle><date>1991-12-01</date><risdate>1991</risdate><volume>27A</volume><issue>12</issue><spage>939</spage><epage>948</epage><pages>939-948</pages><issn>0883-8364</issn><eissn>2327-431X</eissn><eissn>1543-706X</eissn><coden>ICDBEO</coden><abstract>To study the regulation of human salivary-type gene expression we developed cell culture systems to support the growth and serial cultivation of salivary gland epithelial and fibroblastic cell types. We have established 22 independent salivary gland epithelial cell strains from parotid or submandibular glands of human or macaque origin. Nineteen strains were derived from normal tissues and three from human parotid gland tumors. Both the normal and the tumor-derived salivary gland epithelial cells could be serially cultivated with the aid of a 3T3 fibroblast feeder layer in a mixture of Ham's F12 and Dulbecco's modified Eagle's media supplemented with fetal bovine serum, calcium, cholera toxin, hydrocortisone, insulin, and epidermal growth factor. Salivary gland epithelial cells cultured under these conditions continued to express the genes for at least two acinar-cell-specific markers at early passages. Amylase enzyme activity was detected in conditioned media from cultured rhesus parotid epithelial cells as late as Passage 5. Proline-rich-protein-specific RNAs were detected in primary cultures of both rhesus and human parotid epithelial cells. Neither amylase enzyme activity nor PRP-specific RNAs were detected in fibroblasts isolated from the same tissues. In addition, salivary gland epithelial cells cultured under our conditions retain the capacity to undergo dramatic morphologic changes in response to different substrata. The cultured salivary gland epithelial cells we have established will be important tools for the study of salivary gland differentiation and the tissue-specific regulation of salivary-type gene expression.</abstract><cop>Largo, MD</cop><pub>Tissue Culture Association, Inc</pub><pmid>1721908</pmid><doi>10.1007/bf02631121</doi><tpages>10</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0883-8364 |
| ispartof | In Vitro Cellular & Developmental Biology - Animal, 1991-12, Vol.27A (12), p.939-948 |
| issn | 0883-8364 2327-431X 1543-706X |
| language | eng |
| recordid | cdi_crossref_primary_10_1007_BF02631121 |
| source | MEDLINE; SpringerLink Journals; Jstor Complete Legacy |
| subjects | Acinar cells Amylases - genetics Amylases - metabolism Animal cells Animals Biological and medical sciences Biotechnology Blotting, Northern Calcium - pharmacology Cell Division - drug effects Cell growth Cell lines Cells, Cultured Cholera Toxin - pharmacology Cultured cells Epidermal Growth Factor - pharmacology Epithelial Cells Epithelium - drug effects Epithelium - enzymology Establishment of new cell lines, improvement of cultural methods, mass cultures Eukaryotic cell cultures Feeder cells Fibroblasts Fundamental and applied biological sciences. Psychology Gene Expression Regulation, Enzymologic - drug effects Humans Hydrocortisone - pharmacology Insulin - pharmacology Macaca Methods. Procedures. Technologies Parotid gland Parotid Gland - cytology Parotid Gland - drug effects Parotid Gland - enzymology Peptides - genetics Peptides - metabolism Proline-Rich Protein Domains RNA RNA, Messenger - genetics RNA, Messenger - metabolism Salivary glands Salivary Glands - cytology Salivary Glands - drug effects Salivary Glands - enzymology Submandibular Gland - cytology Submandibular Gland - drug effects Submandibular Gland - enzymology Time Factors |
| title | Serial Cultivation of Epithelial Cells from Human and Macaque Salivary Glands |
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