Rapid multiple shoot production from cotyledonary node explants of pea (Pisum sativum L.)
Multiple shoots were produced from cotyledonary node explants of pea (Pisum sativum L.) cultured on MS medium containing$1 mg l^{-1}$BAP. The number of buds formed could be increased by scraping the nodes before culture or by increasing the cytokinin concentration. However, cytokinin levels over$5 m...
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| Veröffentlicht in: | In Vitro Cellular &Developmental Biology 1990-08, Vol.26 (8), p.835-838 |
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| container_issue | 8 |
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| container_title | In Vitro Cellular &Developmental Biology |
| container_volume | 26 |
| creator | Jackson, J.A. (National Research Council of Canada, Saskatoon, Saskatchewan, Canada) Hobbs, S.L.A |
| description | Multiple shoots were produced from cotyledonary node explants of pea (Pisum sativum L.) cultured on MS medium containing$1 mg l^{-1}$BAP. The number of buds formed could be increased by scraping the nodes before culture or by increasing the cytokinin concentration. However, cytokinin levels over$5 mg l^{-1}$increasingly produced shoots that were vitrified and difficult to root. With all the genotypes tested, developing buds were visible as soon as 5 days after culture and elongated shoots could be removed after 21 days. Histological studies indicated that the buds and shoots were formed from superficial layers of tissue. The efficiency of this regeneration system compared favorably with previously published methods. The rapid, genotype-independent, high frequency system described here may be of use in the production of transgenic pea plants. |
| doi_str_mv | 10.1007/bf02623626 |
| format | Article |
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(National Research Council of Canada, Saskatoon, Saskatchewan, Canada) ; Hobbs, S.L.A</creator><creatorcontrib>Jackson, J.A. (National Research Council of Canada, Saskatoon, Saskatchewan, Canada) ; Hobbs, S.L.A</creatorcontrib><description>Multiple shoots were produced from cotyledonary node explants of pea (Pisum sativum L.) cultured on MS medium containing$1 mg l^{-1}$BAP. The number of buds formed could be increased by scraping the nodes before culture or by increasing the cytokinin concentration. However, cytokinin levels over$5 mg l^{-1}$increasingly produced shoots that were vitrified and difficult to root. With all the genotypes tested, developing buds were visible as soon as 5 days after culture and elongated shoots could be removed after 21 days. Histological studies indicated that the buds and shoots were formed from superficial layers of tissue. The efficiency of this regeneration system compared favorably with previously published methods. The rapid, genotype-independent, high frequency system described here may be of use in the production of transgenic pea plants.</description><identifier>ISSN: 0883-8364</identifier><identifier>EISSN: 2327-431X</identifier><identifier>EISSN: 1475-2689</identifier><identifier>DOI: 10.1007/bf02623626</identifier><identifier>CODEN: ICDBEO</identifier><language>eng</language><publisher>Largo, MD: Tissue Culture Association, Inc</publisher><subject>6-BENZILADENINA ; 6-BENZYLADENINE ; Biological and medical sciences ; Biotechnology ; Callus ; COTILEDONES ; COTYLEDON ; COTYLEDONS ; CULTURE MEDIA ; Cytokinins ; Eukaryotic cell cultures ; EXPLANT ; EXPLANTES ; EXPLANTS ; Folktales ; Fundamental and applied biological sciences. Psychology ; Genotypes ; In vitro propagation: entire plant regeneration from tissues and cell cultures ; MEDIO DE CULTIVO ; Methods. Procedures. Technologies ; MILIEU DE CULTURE ; Organogenesis ; Peas ; PISUM SATIVUM ; Plant cells ; Plant cells and fungal cells ; Plant Cellular and Developmental Biology ; Plants ; Plumule ; REGENERACION ; REGENERATION</subject><ispartof>In Vitro Cellular &Developmental Biology, 1990-08, Vol.26 (8), p.835-838</ispartof><rights>Copyright 1990 Tissue Culture Association</rights><rights>1991 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c280t-b7c6c3a75b23308a9a09285b506c3f0dbc625ff936227c5b1353edc2dc21fd713</citedby><cites>FETCH-LOGICAL-c280t-b7c6c3a75b23308a9a09285b506c3f0dbc625ff936227c5b1353edc2dc21fd713</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/4296520$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/4296520$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,776,780,799,27901,27902,57992,58225</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=19838553$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>Jackson, J.A. (National Research Council of Canada, Saskatoon, Saskatchewan, Canada)</creatorcontrib><creatorcontrib>Hobbs, S.L.A</creatorcontrib><title>Rapid multiple shoot production from cotyledonary node explants of pea (Pisum sativum L.)</title><title>In Vitro Cellular &Developmental Biology</title><description>Multiple shoots were produced from cotyledonary node explants of pea (Pisum sativum L.) cultured on MS medium containing$1 mg l^{-1}$BAP. The number of buds formed could be increased by scraping the nodes before culture or by increasing the cytokinin concentration. However, cytokinin levels over$5 mg l^{-1}$increasingly produced shoots that were vitrified and difficult to root. With all the genotypes tested, developing buds were visible as soon as 5 days after culture and elongated shoots could be removed after 21 days. Histological studies indicated that the buds and shoots were formed from superficial layers of tissue. The efficiency of this regeneration system compared favorably with previously published methods. The rapid, genotype-independent, high frequency system described here may be of use in the production of transgenic pea plants.</description><subject>6-BENZILADENINA</subject><subject>6-BENZYLADENINE</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Callus</subject><subject>COTILEDONES</subject><subject>COTYLEDON</subject><subject>COTYLEDONS</subject><subject>CULTURE MEDIA</subject><subject>Cytokinins</subject><subject>Eukaryotic cell cultures</subject><subject>EXPLANT</subject><subject>EXPLANTES</subject><subject>EXPLANTS</subject><subject>Folktales</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genotypes</subject><subject>In vitro propagation: entire plant regeneration from tissues and cell cultures</subject><subject>MEDIO DE CULTIVO</subject><subject>Methods. Procedures. Technologies</subject><subject>MILIEU DE CULTURE</subject><subject>Organogenesis</subject><subject>Peas</subject><subject>PISUM SATIVUM</subject><subject>Plant cells</subject><subject>Plant cells and fungal cells</subject><subject>Plant Cellular and Developmental Biology</subject><subject>Plants</subject><subject>Plumule</subject><subject>REGENERACION</subject><subject>REGENERATION</subject><issn>0883-8364</issn><issn>2327-431X</issn><issn>1475-2689</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1990</creationdate><recordtype>article</recordtype><recordid>eNpFUE1LxDAQDaLgunrxKB5yEVTomk42aXrUxVVhQVEX9FTSNNEubVOSrLj_3kj9gIEH8968mXkIHaZkkhKSXZSGAAfKgW-hEVDIkilNX7bRiAhBE0H5dBfteb8ihBIOMEKvj7KvK9yum1D3jcb-3dqAe2ertQq17bBxtsXKhk2jK9tJt8GdrTTWn30ju-CxNbjXEp8-1H7dYi9D_RFxMTnbRztGNl4f_OAYLefXz7PbZHF_cze7XCQKBAlJmSmuqMxYCZQSIXNJchCsZCS2DalKxYEZk8efIFOsTCmjulIQKzVVltIxOh98lbPeO22K3tVtPLRISfEdSnE1_w0lik8GcS-9ko1xslO1_5_IBRWM0ag7HnQrH6z746eQcwYk0kcDbaQt5JuLFsunPC7jBOgXo5Zy-Q</recordid><startdate>19900801</startdate><enddate>19900801</enddate><creator>Jackson, J.A. (National Research Council of Canada, Saskatoon, Saskatchewan, Canada)</creator><creator>Hobbs, S.L.A</creator><general>Tissue Culture Association, Inc</general><general>Society for In Vitro Biology</general><scope>FBQ</scope><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>19900801</creationdate><title>Rapid multiple shoot production from cotyledonary node explants of pea (Pisum sativum L.)</title><author>Jackson, J.A. (National Research Council of Canada, Saskatoon, Saskatchewan, Canada) ; Hobbs, S.L.A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c280t-b7c6c3a75b23308a9a09285b506c3f0dbc625ff936227c5b1353edc2dc21fd713</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1990</creationdate><topic>6-BENZILADENINA</topic><topic>6-BENZYLADENINE</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>Callus</topic><topic>COTILEDONES</topic><topic>COTYLEDON</topic><topic>COTYLEDONS</topic><topic>CULTURE MEDIA</topic><topic>Cytokinins</topic><topic>Eukaryotic cell cultures</topic><topic>EXPLANT</topic><topic>EXPLANTES</topic><topic>EXPLANTS</topic><topic>Folktales</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genotypes</topic><topic>In vitro propagation: entire plant regeneration from tissues and cell cultures</topic><topic>MEDIO DE CULTIVO</topic><topic>Methods. Procedures. Technologies</topic><topic>MILIEU DE CULTURE</topic><topic>Organogenesis</topic><topic>Peas</topic><topic>PISUM SATIVUM</topic><topic>Plant cells</topic><topic>Plant cells and fungal cells</topic><topic>Plant Cellular and Developmental Biology</topic><topic>Plants</topic><topic>Plumule</topic><topic>REGENERACION</topic><topic>REGENERATION</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Jackson, J.A. (National Research Council of Canada, Saskatoon, Saskatchewan, Canada)</creatorcontrib><creatorcontrib>Hobbs, S.L.A</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>CrossRef</collection><jtitle>In Vitro Cellular &Developmental Biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Jackson, J.A. (National Research Council of Canada, Saskatoon, Saskatchewan, Canada)</au><au>Hobbs, S.L.A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Rapid multiple shoot production from cotyledonary node explants of pea (Pisum sativum L.)</atitle><jtitle>In Vitro Cellular &Developmental Biology</jtitle><date>1990-08-01</date><risdate>1990</risdate><volume>26</volume><issue>8</issue><spage>835</spage><epage>838</epage><pages>835-838</pages><issn>0883-8364</issn><eissn>2327-431X</eissn><eissn>1475-2689</eissn><coden>ICDBEO</coden><abstract>Multiple shoots were produced from cotyledonary node explants of pea (Pisum sativum L.) cultured on MS medium containing$1 mg l^{-1}$BAP. The number of buds formed could be increased by scraping the nodes before culture or by increasing the cytokinin concentration. However, cytokinin levels over$5 mg l^{-1}$increasingly produced shoots that were vitrified and difficult to root. With all the genotypes tested, developing buds were visible as soon as 5 days after culture and elongated shoots could be removed after 21 days. Histological studies indicated that the buds and shoots were formed from superficial layers of tissue. The efficiency of this regeneration system compared favorably with previously published methods. The rapid, genotype-independent, high frequency system described here may be of use in the production of transgenic pea plants.</abstract><cop>Largo, MD</cop><pub>Tissue Culture Association, Inc</pub><doi>10.1007/bf02623626</doi><tpages>4</tpages></addata></record> |
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| identifier | ISSN: 0883-8364 |
| ispartof | In Vitro Cellular &Developmental Biology, 1990-08, Vol.26 (8), p.835-838 |
| issn | 0883-8364 2327-431X 1475-2689 |
| language | eng |
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| source | Jstor Complete Legacy; SpringerLink Journals - AutoHoldings |
| subjects | 6-BENZILADENINA 6-BENZYLADENINE Biological and medical sciences Biotechnology Callus COTILEDONES COTYLEDON COTYLEDONS CULTURE MEDIA Cytokinins Eukaryotic cell cultures EXPLANT EXPLANTES EXPLANTS Folktales Fundamental and applied biological sciences. Psychology Genotypes In vitro propagation: entire plant regeneration from tissues and cell cultures MEDIO DE CULTIVO Methods. Procedures. Technologies MILIEU DE CULTURE Organogenesis Peas PISUM SATIVUM Plant cells Plant cells and fungal cells Plant Cellular and Developmental Biology Plants Plumule REGENERACION REGENERATION |
| title | Rapid multiple shoot production from cotyledonary node explants of pea (Pisum sativum L.) |
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