Separation and identification of ceramides in the human stratum corneum by high-performance liquid chromatography coupled with electrospray ionization mass spectrometry and electrospray multiple-stage mass spectrometry profiling
A sensitive, selective, and rapid method is described for analysis of ceramides in the human stratum coracum by direct coupling of HPLC with an electrospray ion-trap mass spectrometry. Nonaqueous reversed-phase chromatography stabilizes the electrospray ionization, resulting in sensitivity that enab...
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| Veröffentlicht in: | Chromatographia 1999-07, Vol.50 (1-2), p.15-20 |
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| creator | Vietzke, J. -P Straßner, M Hintze, U |
| description | A sensitive, selective, and rapid method is described for analysis of ceramides in the human stratum coracum by direct coupling of HPLC with an electrospray ion-trap mass spectrometry. Nonaqueous reversed-phase chromatography stabilizes the electrospray ionization, resulting in sensitivity that enables direct measurement of skin lipid extracts with no special sample preparation. Assignment of individual signals to the corresponding ceramide species is based on interpretation of the fragment spectra from MS-MS experiments. This enables much finer differentiation between ceramdies than that achievable by thin-layer chromatography.A sensitive, selective, and rapid method is described for analysis of ceramides in the human stratum corneum by direct coupling of HPLC with an electrospray ion-trap mass spectrometry. Nonaqueous reversed-phase chromatography stabilizes the electrospray ionization, resulting in sensitivity that enables direct measurement of skin lipid extracts with no special sample preparation. Assignment of individual signals to the corresponding ceramide species is based on interpretation of the fragment spectra from MS-MS experiments. This enables much finer differentiation between ceramides than that achievable by thin-layer chromatography. |
| doi_str_mv | 10.1007/BF02493611 |
| format | Article |
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Nonaqueous reversed-phase chromatography stabilizes the electrospray ionization, resulting in sensitivity that enables direct measurement of skin lipid extracts with no special sample preparation. Assignment of individual signals to the corresponding ceramide species is based on interpretation of the fragment spectra from MS-MS experiments. This enables much finer differentiation between ceramdies than that achievable by thin-layer chromatography.A sensitive, selective, and rapid method is described for analysis of ceramides in the human stratum corneum by direct coupling of HPLC with an electrospray ion-trap mass spectrometry. Nonaqueous reversed-phase chromatography stabilizes the electrospray ionization, resulting in sensitivity that enables direct measurement of skin lipid extracts with no special sample preparation. Assignment of individual signals to the corresponding ceramide species is based on interpretation of the fragment spectra from MS-MS experiments. This enables much finer differentiation between ceramides than that achievable by thin-layer chromatography.</description><identifier>ISSN: 0009-5893</identifier><identifier>EISSN: 1612-1112</identifier><identifier>DOI: 10.1007/BF02493611</identifier><identifier>CODEN: CHRGB7</identifier><language>eng</language><publisher>Oxford: Vieweg Verlag</publisher><subject>Biological and medical sciences ; ceramides ; Dermatology ; electrospray ionization mass spectrometry ; high performance liquid chromatography ; humans ; Investigative techniques, diagnostic techniques (general aspects) ; ionization ; Medical sciences ; Pathology. Cytology. Biochemistry. Spectrometry. 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Nonaqueous reversed-phase chromatography stabilizes the electrospray ionization, resulting in sensitivity that enables direct measurement of skin lipid extracts with no special sample preparation. Assignment of individual signals to the corresponding ceramide species is based on interpretation of the fragment spectra from MS-MS experiments. This enables much finer differentiation between ceramdies than that achievable by thin-layer chromatography.A sensitive, selective, and rapid method is described for analysis of ceramides in the human stratum corneum by direct coupling of HPLC with an electrospray ion-trap mass spectrometry. Nonaqueous reversed-phase chromatography stabilizes the electrospray ionization, resulting in sensitivity that enables direct measurement of skin lipid extracts with no special sample preparation. Assignment of individual signals to the corresponding ceramide species is based on interpretation of the fragment spectra from MS-MS experiments. This enables much finer differentiation between ceramides than that achievable by thin-layer chromatography.</description><subject>Biological and medical sciences</subject><subject>ceramides</subject><subject>Dermatology</subject><subject>electrospray ionization mass spectrometry</subject><subject>high performance liquid chromatography</subject><subject>humans</subject><subject>Investigative techniques, diagnostic techniques (general aspects)</subject><subject>ionization</subject><subject>Medical sciences</subject><subject>Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques</subject><subject>rapid methods</subject><subject>reversed-phase liquid chromatography</subject><subject>thin layer chromatography</subject><issn>0009-5893</issn><issn>1612-1112</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><recordid>eNptkd-K1TAQxoMoeDx64wuYC6-Eav6c9jSXuri6sODF0esyTSZtpG1ikiL1eX0Q41ZYhJ2bYSa_b2bIR8hLzt5yxs7vPlwzcVKy4fwROfCGi4pzLh6TA2NMVXWr5FPyLKXvpRSqaQ7k9wUDRMjOLxQWQ53BJTvr9N7ylmqMMJd2om6heUQ6rjMsNOWiWmeqfVyw5H6joxvGKmC0PhZCI53cj9UZqsfoZ8h-iBDGrSjWMKGhP10eKU6oc_QpRNho2eh-7YtnSImmcPc4Y47b3XX_0fM6ZVcmVSnDgA8oQvTWTW4ZnpMnFqaEL_7lI7lcf_x69bm6_fLp5ur9baVFK3IlhWmssi3vVdNrQHtW0PZWCVDmXCPUaDn24lQzLaSRrWEgG3PmuocToDySN_tUXS5MEW0Xopshbh1n3V93unt3Cvx6hwMkDZON5cNcule0bS1LHMmrHbPgOxhiQb5dBONNMfBUS8blH0W9omA</recordid><startdate>19990701</startdate><enddate>19990701</enddate><creator>Vietzke, J. -P</creator><creator>Straßner, M</creator><creator>Hintze, U</creator><general>Vieweg Verlag</general><general>Springer</general><scope>FBQ</scope><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>19990701</creationdate><title>Separation and identification of ceramides in the human stratum corneum by high-performance liquid chromatography coupled with electrospray ionization mass spectrometry and electrospray multiple-stage mass spectrometry profiling</title><author>Vietzke, J. -P ; Straßner, M ; Hintze, U</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c282t-32d6f9f81b96bcaef79a8bf92a9d75ea5ef1eb2450c23d38d0a36d71cba4ae3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Biological and medical sciences</topic><topic>ceramides</topic><topic>Dermatology</topic><topic>electrospray ionization mass spectrometry</topic><topic>high performance liquid chromatography</topic><topic>humans</topic><topic>Investigative techniques, diagnostic techniques (general aspects)</topic><topic>ionization</topic><topic>Medical sciences</topic><topic>Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques</topic><topic>rapid methods</topic><topic>reversed-phase liquid chromatography</topic><topic>thin layer chromatography</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Vietzke, J. -P</creatorcontrib><creatorcontrib>Straßner, M</creatorcontrib><creatorcontrib>Hintze, U</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>CrossRef</collection><jtitle>Chromatographia</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Vietzke, J. -P</au><au>Straßner, M</au><au>Hintze, U</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Separation and identification of ceramides in the human stratum corneum by high-performance liquid chromatography coupled with electrospray ionization mass spectrometry and electrospray multiple-stage mass spectrometry profiling</atitle><jtitle>Chromatographia</jtitle><date>1999-07-01</date><risdate>1999</risdate><volume>50</volume><issue>1-2</issue><spage>15</spage><epage>20</epage><pages>15-20</pages><issn>0009-5893</issn><eissn>1612-1112</eissn><coden>CHRGB7</coden><abstract>A sensitive, selective, and rapid method is described for analysis of ceramides in the human stratum coracum by direct coupling of HPLC with an electrospray ion-trap mass spectrometry. Nonaqueous reversed-phase chromatography stabilizes the electrospray ionization, resulting in sensitivity that enables direct measurement of skin lipid extracts with no special sample preparation. Assignment of individual signals to the corresponding ceramide species is based on interpretation of the fragment spectra from MS-MS experiments. This enables much finer differentiation between ceramdies than that achievable by thin-layer chromatography.A sensitive, selective, and rapid method is described for analysis of ceramides in the human stratum corneum by direct coupling of HPLC with an electrospray ion-trap mass spectrometry. Nonaqueous reversed-phase chromatography stabilizes the electrospray ionization, resulting in sensitivity that enables direct measurement of skin lipid extracts with no special sample preparation. Assignment of individual signals to the corresponding ceramide species is based on interpretation of the fragment spectra from MS-MS experiments. This enables much finer differentiation between ceramides than that achievable by thin-layer chromatography.</abstract><cop>Oxford</cop><pub>Vieweg Verlag</pub><doi>10.1007/BF02493611</doi><tpages>6</tpages></addata></record> |
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| identifier | ISSN: 0009-5893 |
| ispartof | Chromatographia, 1999-07, Vol.50 (1-2), p.15-20 |
| issn | 0009-5893 1612-1112 |
| language | eng |
| recordid | cdi_crossref_primary_10_1007_BF02493611 |
| source | SpringerNature Journals |
| subjects | Biological and medical sciences ceramides Dermatology electrospray ionization mass spectrometry high performance liquid chromatography humans Investigative techniques, diagnostic techniques (general aspects) ionization Medical sciences Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques rapid methods reversed-phase liquid chromatography thin layer chromatography |
| title | Separation and identification of ceramides in the human stratum corneum by high-performance liquid chromatography coupled with electrospray ionization mass spectrometry and electrospray multiple-stage mass spectrometry profiling |
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