Separation and identification of ceramides in the human stratum corneum by high-performance liquid chromatography coupled with electrospray ionization mass spectrometry and electrospray multiple-stage mass spectrometry profiling

A sensitive, selective, and rapid method is described for analysis of ceramides in the human stratum coracum by direct coupling of HPLC with an electrospray ion-trap mass spectrometry. Nonaqueous reversed-phase chromatography stabilizes the electrospray ionization, resulting in sensitivity that enab...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Chromatographia 1999-07, Vol.50 (1-2), p.15-20
Hauptverfasser: Vietzke, J. -P, Straßner, M, Hintze, U
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page 20
container_issue 1-2
container_start_page 15
container_title Chromatographia
container_volume 50
creator Vietzke, J. -P
Straßner, M
Hintze, U
description A sensitive, selective, and rapid method is described for analysis of ceramides in the human stratum coracum by direct coupling of HPLC with an electrospray ion-trap mass spectrometry. Nonaqueous reversed-phase chromatography stabilizes the electrospray ionization, resulting in sensitivity that enables direct measurement of skin lipid extracts with no special sample preparation. Assignment of individual signals to the corresponding ceramide species is based on interpretation of the fragment spectra from MS-MS experiments. This enables much finer differentiation between ceramdies than that achievable by thin-layer chromatography.A sensitive, selective, and rapid method is described for analysis of ceramides in the human stratum corneum by direct coupling of HPLC with an electrospray ion-trap mass spectrometry. Nonaqueous reversed-phase chromatography stabilizes the electrospray ionization, resulting in sensitivity that enables direct measurement of skin lipid extracts with no special sample preparation. Assignment of individual signals to the corresponding ceramide species is based on interpretation of the fragment spectra from MS-MS experiments. This enables much finer differentiation between ceramides than that achievable by thin-layer chromatography.
doi_str_mv 10.1007/BF02493611
format Article
fullrecord <record><control><sourceid>fao_cross</sourceid><recordid>TN_cdi_crossref_primary_10_1007_BF02493611</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>US201600045301</sourcerecordid><originalsourceid>FETCH-LOGICAL-c282t-32d6f9f81b96bcaef79a8bf92a9d75ea5ef1eb2450c23d38d0a36d71cba4ae3</originalsourceid><addsrcrecordid>eNptkd-K1TAQxoMoeDx64wuYC6-Eav6c9jSXuri6sODF0esyTSZtpG1ikiL1eX0Q41ZYhJ2bYSa_b2bIR8hLzt5yxs7vPlwzcVKy4fwROfCGi4pzLh6TA2NMVXWr5FPyLKXvpRSqaQ7k9wUDRMjOLxQWQ53BJTvr9N7ylmqMMJd2om6heUQ6rjMsNOWiWmeqfVyw5H6joxvGKmC0PhZCI53cj9UZqsfoZ8h-iBDGrSjWMKGhP10eKU6oc_QpRNho2eh-7YtnSImmcPc4Y47b3XX_0fM6ZVcmVSnDgA8oQvTWTW4ZnpMnFqaEL_7lI7lcf_x69bm6_fLp5ur9baVFK3IlhWmssi3vVdNrQHtW0PZWCVDmXCPUaDn24lQzLaSRrWEgG3PmuocToDySN_tUXS5MEW0Xopshbh1n3V93unt3Cvx6hwMkDZON5cNcule0bS1LHMmrHbPgOxhiQb5dBONNMfBUS8blH0W9omA</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>Separation and identification of ceramides in the human stratum corneum by high-performance liquid chromatography coupled with electrospray ionization mass spectrometry and electrospray multiple-stage mass spectrometry profiling</title><source>SpringerNature Journals</source><creator>Vietzke, J. -P ; Straßner, M ; Hintze, U</creator><creatorcontrib>Vietzke, J. -P ; Straßner, M ; Hintze, U</creatorcontrib><description>A sensitive, selective, and rapid method is described for analysis of ceramides in the human stratum coracum by direct coupling of HPLC with an electrospray ion-trap mass spectrometry. Nonaqueous reversed-phase chromatography stabilizes the electrospray ionization, resulting in sensitivity that enables direct measurement of skin lipid extracts with no special sample preparation. Assignment of individual signals to the corresponding ceramide species is based on interpretation of the fragment spectra from MS-MS experiments. This enables much finer differentiation between ceramdies than that achievable by thin-layer chromatography.A sensitive, selective, and rapid method is described for analysis of ceramides in the human stratum corneum by direct coupling of HPLC with an electrospray ion-trap mass spectrometry. Nonaqueous reversed-phase chromatography stabilizes the electrospray ionization, resulting in sensitivity that enables direct measurement of skin lipid extracts with no special sample preparation. Assignment of individual signals to the corresponding ceramide species is based on interpretation of the fragment spectra from MS-MS experiments. This enables much finer differentiation between ceramides than that achievable by thin-layer chromatography.</description><identifier>ISSN: 0009-5893</identifier><identifier>EISSN: 1612-1112</identifier><identifier>DOI: 10.1007/BF02493611</identifier><identifier>CODEN: CHRGB7</identifier><language>eng</language><publisher>Oxford: Vieweg Verlag</publisher><subject>Biological and medical sciences ; ceramides ; Dermatology ; electrospray ionization mass spectrometry ; high performance liquid chromatography ; humans ; Investigative techniques, diagnostic techniques (general aspects) ; ionization ; Medical sciences ; Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques ; rapid methods ; reversed-phase liquid chromatography ; thin layer chromatography</subject><ispartof>Chromatographia, 1999-07, Vol.50 (1-2), p.15-20</ispartof><rights>1999 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c282t-32d6f9f81b96bcaef79a8bf92a9d75ea5ef1eb2450c23d38d0a36d71cba4ae3</citedby><cites>FETCH-LOGICAL-c282t-32d6f9f81b96bcaef79a8bf92a9d75ea5ef1eb2450c23d38d0a36d71cba4ae3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,782,786,27931,27932</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&amp;idt=1885333$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>Vietzke, J. -P</creatorcontrib><creatorcontrib>Straßner, M</creatorcontrib><creatorcontrib>Hintze, U</creatorcontrib><title>Separation and identification of ceramides in the human stratum corneum by high-performance liquid chromatography coupled with electrospray ionization mass spectrometry and electrospray multiple-stage mass spectrometry profiling</title><title>Chromatographia</title><description>A sensitive, selective, and rapid method is described for analysis of ceramides in the human stratum coracum by direct coupling of HPLC with an electrospray ion-trap mass spectrometry. Nonaqueous reversed-phase chromatography stabilizes the electrospray ionization, resulting in sensitivity that enables direct measurement of skin lipid extracts with no special sample preparation. Assignment of individual signals to the corresponding ceramide species is based on interpretation of the fragment spectra from MS-MS experiments. This enables much finer differentiation between ceramdies than that achievable by thin-layer chromatography.A sensitive, selective, and rapid method is described for analysis of ceramides in the human stratum corneum by direct coupling of HPLC with an electrospray ion-trap mass spectrometry. Nonaqueous reversed-phase chromatography stabilizes the electrospray ionization, resulting in sensitivity that enables direct measurement of skin lipid extracts with no special sample preparation. Assignment of individual signals to the corresponding ceramide species is based on interpretation of the fragment spectra from MS-MS experiments. This enables much finer differentiation between ceramides than that achievable by thin-layer chromatography.</description><subject>Biological and medical sciences</subject><subject>ceramides</subject><subject>Dermatology</subject><subject>electrospray ionization mass spectrometry</subject><subject>high performance liquid chromatography</subject><subject>humans</subject><subject>Investigative techniques, diagnostic techniques (general aspects)</subject><subject>ionization</subject><subject>Medical sciences</subject><subject>Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques</subject><subject>rapid methods</subject><subject>reversed-phase liquid chromatography</subject><subject>thin layer chromatography</subject><issn>0009-5893</issn><issn>1612-1112</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><recordid>eNptkd-K1TAQxoMoeDx64wuYC6-Eav6c9jSXuri6sODF0esyTSZtpG1ikiL1eX0Q41ZYhJ2bYSa_b2bIR8hLzt5yxs7vPlwzcVKy4fwROfCGi4pzLh6TA2NMVXWr5FPyLKXvpRSqaQ7k9wUDRMjOLxQWQ53BJTvr9N7ylmqMMJd2om6heUQ6rjMsNOWiWmeqfVyw5H6joxvGKmC0PhZCI53cj9UZqsfoZ8h-iBDGrSjWMKGhP10eKU6oc_QpRNho2eh-7YtnSImmcPc4Y47b3XX_0fM6ZVcmVSnDgA8oQvTWTW4ZnpMnFqaEL_7lI7lcf_x69bm6_fLp5ur9baVFK3IlhWmssi3vVdNrQHtW0PZWCVDmXCPUaDn24lQzLaSRrWEgG3PmuocToDySN_tUXS5MEW0Xopshbh1n3V93unt3Cvx6hwMkDZON5cNcule0bS1LHMmrHbPgOxhiQb5dBONNMfBUS8blH0W9omA</recordid><startdate>19990701</startdate><enddate>19990701</enddate><creator>Vietzke, J. -P</creator><creator>Straßner, M</creator><creator>Hintze, U</creator><general>Vieweg Verlag</general><general>Springer</general><scope>FBQ</scope><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>19990701</creationdate><title>Separation and identification of ceramides in the human stratum corneum by high-performance liquid chromatography coupled with electrospray ionization mass spectrometry and electrospray multiple-stage mass spectrometry profiling</title><author>Vietzke, J. -P ; Straßner, M ; Hintze, U</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c282t-32d6f9f81b96bcaef79a8bf92a9d75ea5ef1eb2450c23d38d0a36d71cba4ae3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Biological and medical sciences</topic><topic>ceramides</topic><topic>Dermatology</topic><topic>electrospray ionization mass spectrometry</topic><topic>high performance liquid chromatography</topic><topic>humans</topic><topic>Investigative techniques, diagnostic techniques (general aspects)</topic><topic>ionization</topic><topic>Medical sciences</topic><topic>Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques</topic><topic>rapid methods</topic><topic>reversed-phase liquid chromatography</topic><topic>thin layer chromatography</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Vietzke, J. -P</creatorcontrib><creatorcontrib>Straßner, M</creatorcontrib><creatorcontrib>Hintze, U</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>CrossRef</collection><jtitle>Chromatographia</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Vietzke, J. -P</au><au>Straßner, M</au><au>Hintze, U</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Separation and identification of ceramides in the human stratum corneum by high-performance liquid chromatography coupled with electrospray ionization mass spectrometry and electrospray multiple-stage mass spectrometry profiling</atitle><jtitle>Chromatographia</jtitle><date>1999-07-01</date><risdate>1999</risdate><volume>50</volume><issue>1-2</issue><spage>15</spage><epage>20</epage><pages>15-20</pages><issn>0009-5893</issn><eissn>1612-1112</eissn><coden>CHRGB7</coden><abstract>A sensitive, selective, and rapid method is described for analysis of ceramides in the human stratum coracum by direct coupling of HPLC with an electrospray ion-trap mass spectrometry. Nonaqueous reversed-phase chromatography stabilizes the electrospray ionization, resulting in sensitivity that enables direct measurement of skin lipid extracts with no special sample preparation. Assignment of individual signals to the corresponding ceramide species is based on interpretation of the fragment spectra from MS-MS experiments. This enables much finer differentiation between ceramdies than that achievable by thin-layer chromatography.A sensitive, selective, and rapid method is described for analysis of ceramides in the human stratum corneum by direct coupling of HPLC with an electrospray ion-trap mass spectrometry. Nonaqueous reversed-phase chromatography stabilizes the electrospray ionization, resulting in sensitivity that enables direct measurement of skin lipid extracts with no special sample preparation. Assignment of individual signals to the corresponding ceramide species is based on interpretation of the fragment spectra from MS-MS experiments. This enables much finer differentiation between ceramides than that achievable by thin-layer chromatography.</abstract><cop>Oxford</cop><pub>Vieweg Verlag</pub><doi>10.1007/BF02493611</doi><tpages>6</tpages></addata></record>
fulltext fulltext
identifier ISSN: 0009-5893
ispartof Chromatographia, 1999-07, Vol.50 (1-2), p.15-20
issn 0009-5893
1612-1112
language eng
recordid cdi_crossref_primary_10_1007_BF02493611
source SpringerNature Journals
subjects Biological and medical sciences
ceramides
Dermatology
electrospray ionization mass spectrometry
high performance liquid chromatography
humans
Investigative techniques, diagnostic techniques (general aspects)
ionization
Medical sciences
Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques
rapid methods
reversed-phase liquid chromatography
thin layer chromatography
title Separation and identification of ceramides in the human stratum corneum by high-performance liquid chromatography coupled with electrospray ionization mass spectrometry and electrospray multiple-stage mass spectrometry profiling
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-06T08%3A10%3A31IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-fao_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Separation%20and%20identification%20of%20ceramides%20in%20the%20human%20stratum%20corneum%20by%20high-performance%20liquid%20chromatography%20coupled%20with%20electrospray%20ionization%20mass%20spectrometry%20and%20electrospray%20multiple-stage%20mass%20spectrometry%20profiling&rft.jtitle=Chromatographia&rft.au=Vietzke,%20J.%20-P&rft.date=1999-07-01&rft.volume=50&rft.issue=1-2&rft.spage=15&rft.epage=20&rft.pages=15-20&rft.issn=0009-5893&rft.eissn=1612-1112&rft.coden=CHRGB7&rft_id=info:doi/10.1007/BF02493611&rft_dat=%3Cfao_cross%3EUS201600045301%3C/fao_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/&rfr_iscdi=true