Effect of chloroquine on mast cell membranes
The effect of chloroquine (CQ) on phospholipid turnover and de novo synthesis in isolated rat mast cells (IRMC) was studied by determining the incorporation of 32P and 14C-glycerol into IRMC phospholipids. Incubation of mast cells with chloroquine increased 32P incorporation into PI and PS whilst it...
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| Veröffentlicht in: | Agents and Actions 1994-06, Vol.41 Spec No (S1), p.C43-C44 |
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| container_title | Agents and Actions |
| container_volume | 41 Spec No |
| creator | Pecivová, J Drábiková, K Nosál, R |
| description | The effect of chloroquine (CQ) on phospholipid turnover and de novo synthesis in isolated rat mast cells (IRMC) was studied by determining the incorporation of 32P and 14C-glycerol into IRMC phospholipids. Incubation of mast cells with chloroquine increased 32P incorporation into PI and PS whilst it decreased 32P incorporation into PC, PE and PA. In mast cells pretreated with CQ and subsequently stimulated with compound 48/80, 32P incorporation into PI, PS and PA fractions was enhanced, while it was decreased into PC and PE, in comparison to 48/80 stimulated IRMC. 14C-glycerol incorporation into total IRMC phospholipids was not significantly changed by CQ and compound 48/80 treatment and neither was any dose-dependent effect of CQ on individual phospholipids detected. Our results indicate that chloroquine, similarly to other cationic amphiphilic drugs, may alter membrane PL turnover without changing de novo synthesis of phospholipids. |
| doi_str_mv | 10.1007/BF02007759 |
| format | Article |
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Incubation of mast cells with chloroquine increased 32P incorporation into PI and PS whilst it decreased 32P incorporation into PC, PE and PA. In mast cells pretreated with CQ and subsequently stimulated with compound 48/80, 32P incorporation into PI, PS and PA fractions was enhanced, while it was decreased into PC and PE, in comparison to 48/80 stimulated IRMC. 14C-glycerol incorporation into total IRMC phospholipids was not significantly changed by CQ and compound 48/80 treatment and neither was any dose-dependent effect of CQ on individual phospholipids detected. Our results indicate that chloroquine, similarly to other cationic amphiphilic drugs, may alter membrane PL turnover without changing de novo synthesis of phospholipids.</description><identifier>ISSN: 0065-4299</identifier><identifier>EISSN: 1420-908X</identifier><identifier>DOI: 10.1007/BF02007759</identifier><identifier>PMID: 7976803</identifier><language>eng</language><publisher>Switzerland</publisher><subject>Animals ; Cell Membrane - drug effects ; Cell Membrane - metabolism ; Chloroquine - pharmacology ; In Vitro Techniques ; Male ; Mast Cells - drug effects ; Mast Cells - metabolism ; Mast Cells - ultrastructure ; p-Methoxy-N-methylphenethylamine - pharmacology ; Phospholipids - metabolism ; Rats ; Rats, Wistar</subject><ispartof>Agents and Actions, 1994-06, Vol.41 Spec No (S1), p.C43-C44</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c282t-35697da8276f510b2a1b720424752efc67d5819d671edbbba3e5260d15bfa6973</citedby><cites>FETCH-LOGICAL-c282t-35697da8276f510b2a1b720424752efc67d5819d671edbbba3e5260d15bfa6973</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7976803$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Pecivová, J</creatorcontrib><creatorcontrib>Drábiková, K</creatorcontrib><creatorcontrib>Nosál, R</creatorcontrib><title>Effect of chloroquine on mast cell membranes</title><title>Agents and Actions</title><addtitle>Agents Actions</addtitle><description>The effect of chloroquine (CQ) on phospholipid turnover and de novo synthesis in isolated rat mast cells (IRMC) was studied by determining the incorporation of 32P and 14C-glycerol into IRMC phospholipids. 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Our results indicate that chloroquine, similarly to other cationic amphiphilic drugs, may alter membrane PL turnover without changing de novo synthesis of phospholipids.</description><subject>Animals</subject><subject>Cell Membrane - drug effects</subject><subject>Cell Membrane - metabolism</subject><subject>Chloroquine - pharmacology</subject><subject>In Vitro Techniques</subject><subject>Male</subject><subject>Mast Cells - drug effects</subject><subject>Mast Cells - metabolism</subject><subject>Mast Cells - ultrastructure</subject><subject>p-Methoxy-N-methylphenethylamine - pharmacology</subject><subject>Phospholipids - metabolism</subject><subject>Rats</subject><subject>Rats, Wistar</subject><issn>0065-4299</issn><issn>1420-908X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkL9LxDAcxYMo53m6uAudHMTqN0nza9TjToUDFwW3kKQJVtrmbNrB_96WKzq95fMeHx5ClxjuMIC4f9wCGVMwdYSWuCCQK5Afx2gJwFleEKVO0VlKXwCMYYkXaCGU4BLoEt1uQvCuz2LI3Gcdu_g9VK3PYps1JvWZ83WdNb6xnWl9OkcnwdTJX8y5Qu_bzdv6Od-9Pr2sH3a5I5L0OWVcidJIInhgGCwx2AoCBSkEIz44LkomsSq5wL601hrqGeFQYmaDGat0ha4Pu_vJx6deN1WaVEaJOCQ9uheMYDqCNwfQdTGlzge976rGdD8ag56u0f_XjPDVvDrYxpd_6PwF_QWWF1wO</recordid><startdate>199406</startdate><enddate>199406</enddate><creator>Pecivová, J</creator><creator>Drábiková, K</creator><creator>Nosál, R</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>199406</creationdate><title>Effect of chloroquine on mast cell membranes</title><author>Pecivová, J ; Drábiková, K ; Nosál, R</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c282t-35697da8276f510b2a1b720424752efc67d5819d671edbbba3e5260d15bfa6973</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1994</creationdate><topic>Animals</topic><topic>Cell Membrane - drug effects</topic><topic>Cell Membrane - metabolism</topic><topic>Chloroquine - pharmacology</topic><topic>In Vitro Techniques</topic><topic>Male</topic><topic>Mast Cells - drug effects</topic><topic>Mast Cells - metabolism</topic><topic>Mast Cells - ultrastructure</topic><topic>p-Methoxy-N-methylphenethylamine - pharmacology</topic><topic>Phospholipids - metabolism</topic><topic>Rats</topic><topic>Rats, Wistar</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Pecivová, J</creatorcontrib><creatorcontrib>Drábiková, K</creatorcontrib><creatorcontrib>Nosál, R</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Agents and Actions</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Pecivová, J</au><au>Drábiková, K</au><au>Nosál, R</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of chloroquine on mast cell membranes</atitle><jtitle>Agents and Actions</jtitle><addtitle>Agents Actions</addtitle><date>1994-06</date><risdate>1994</risdate><volume>41 Spec No</volume><issue>S1</issue><spage>C43</spage><epage>C44</epage><pages>C43-C44</pages><issn>0065-4299</issn><eissn>1420-908X</eissn><abstract>The effect of chloroquine (CQ) on phospholipid turnover and de novo synthesis in isolated rat mast cells (IRMC) was studied by determining the incorporation of 32P and 14C-glycerol into IRMC phospholipids. 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| ispartof | Agents and Actions, 1994-06, Vol.41 Spec No (S1), p.C43-C44 |
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| source | MEDLINE; Springer Nature - Complete Springer Journals |
| subjects | Animals Cell Membrane - drug effects Cell Membrane - metabolism Chloroquine - pharmacology In Vitro Techniques Male Mast Cells - drug effects Mast Cells - metabolism Mast Cells - ultrastructure p-Methoxy-N-methylphenethylamine - pharmacology Phospholipids - metabolism Rats Rats, Wistar |
| title | Effect of chloroquine on mast cell membranes |
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