Characterization of a tight-binding MMP-3 inhibitor using improved fluorescence spectroscopy techniques

Accurate kinetic characterization of stromelysin (MMP-3) inhibitors is critical in the design of potent inhibitors of this enzyme. We have successfully modified a previously described assay [1] which used an internally quenched peptide substrate (Dnp-PYAYWMR) that, upon cleavage by MMP-3, produces t...

Ausführliche Beschreibung

Bibliographische Detailangaben

Veröffentlicht in:	Agents and Actions 1993-01, Vol.39 Spec No (S1), p.C189-C191
Hauptverfasser:	Finch-Arietta, M, Johnson, W, Lusch, L, Fotouhi, N, Walsky, R, Hanglow, A C
Format:	Artikel
Sprache:	eng
Schlagworte:	Binding Sites Hydroxamic Acids - chemistry Hydroxamic Acids - metabolism Matrix Metalloproteinase 3 Metalloendopeptidases - antagonists & inhibitors Metalloendopeptidases - metabolism Oligopeptides - metabolism Oligopeptides - pharmacology Spectrometry, Fluorescence
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!