Coupling of fructose-1,6-P2 to aminated agarose by Schiff base reduction. Affinity chromatography of yeast aldolase

Fructose-1,6-P2 was immobilized by sodium borohydride reduction of the Schiff base formed with aminated agarose (AH-Sepharose 4B). The coupling occurs with high yield (25 mumoles immobilized fructose-1,6-P2 per ml packed gel) at neutral pH and room temperature. Schiff base reduction thus provides a...

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Veröffentlicht in:	Experientia 1977-06, Vol.33 (6), p.709-711
Hauptverfasser:	Lubini, D, Healy, M J, Christen, P
Format:	Artikel
Sprache:	eng
Schlagworte:	Chemical Phenomena Chemistry Chromatography, Affinity - methods Fructose-Bisphosphate Aldolase - isolation & purification Fructosephosphates Saccharomyces cerevisiae - enzymology Schiff Bases Sepharose
Online-Zugang:	Volltext
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